Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,388,883	T→G	7.0%	L798R (CTG→CGG) ‡	dld2 →	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,388,883	0	T	G	7.0%	290.8 / 9.6	97	L798R (CTG→CGG) ‡	dld2	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (40/50);  new base G (3/4);  total (43/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGG  >  NC_002947/5388741‑5389024                                                                                                                                                 |                                                                                                                                              tgctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGc                                                                                                                                          <  2:176260/148‑1 (MQ=255) tgctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCa                                                                                                                                         <  5:183457/149‑1 (MQ=255) tgctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCa                                                                                                                                         <  8:203194/149‑1 (MQ=255)   ctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAgg                                                                                                                                       >  7:127985/1‑149 (MQ=255)   ctgcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAg                                                                                                                                        <  7:174946/148‑1 (MQ=255)   ctgaGGGCAACCGTTCGCCTCCTAGGGCTACCCCGAGCAGGCCGAACACA‑AAGGCCAGGAGCTGATCACCGCCCTGCTGAACGCCAGACGCGGCTGCCTCGACCCGATCAATTCCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAg                                                                                                                                        <  4:14077/148‑1 (MQ=255)     gcGGCCAACCGTTCGCCTCCAAGGGCTGCCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGa                                                                                                                                      >  6:52828/1‑148 (MQ=255)     gcGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGa                                                                                                                                      <  5:52828/148‑1 (MQ=255)      cGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGa                                                                                                                                      <  7:102985/147‑1 (MQ=255)      cGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGAc                                                                                                                                     <  7:11761/148‑1 (MQ=255)      cGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCA‑GGGTGCAggg                                                                                                                                      <  5:245607/147‑2 (MQ=255)       ggCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCA‑GGGTGCAggggct                                                                                                                                   <  3:5795/149‑5 (MQ=255)         ccAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCt                                                                                                                                   >  8:2929/1‑147 (MQ=255)           aaCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGc                                                                                                                               >  1:120101/1‑149 (MQ=255)           aaCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCA‑GGGTGCAggg                                                                                                                                      <  8:290552/142‑2 (MQ=255)           aaCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCA‑GGGTGCAggg                                                                                                                                      >  7:290552/1‑141 (MQ=255)            aCCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGc                                                                                                                               >  2:200437/1‑148 (MQ=255)             ccGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGa                                                                                                                             <  3:3320/149‑1 (MQ=255)               gTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACCCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGAGCAGGACCTGAGCGaca                                                                                                                           >  1:44391/1‑149 (MQ=255)                ttCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGacac                                                                                                                          >  5:27832/1‑149 (MQ=255)                   gCCTCCAAGGGCTACCCCGAGCAGGCCGAACACATACCGCCAGGAGCTGATCACCCCCCTGCTCCACGCCAGCCGCGGCGGCCTCGACCCGAGCTATTGCGACACAAGCCTCTGCACCCTGCGCC‑Tg                                                                                                                                              >  1:46148/1‑127 (MQ=255)                   gCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCTCTGCACCCTGCGCC‑Tg                                                                                                                                              <  2:46148/126‑1 (MQ=255)                    ccTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCgg                                                                                                                      >  4:208645/1‑149 (MQ=255)                      tCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCt                                                                                                                    >  8:166957/1‑149 (MQ=255)                      tCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACCCCAGCCGCGCCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCt                                                                                                                    >  1:320721/1‑149 (MQ=255)                       ccAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCA‑GGGTGCAggggc                                                                                                                                    >  7:163602/1‑129 (MQ=255)                       ccAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCAGGCGCA‑GGGTGCAggggc                                                                                                                                    <  8:163602/132‑4 (MQ=255)                        cAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTgg                                                                                                                  <  3:208645/149‑1 (MQ=255)                        cAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTg                                                                                                                   >  5:142738/1‑148 (MQ=255)                         aaGGGCTACCCCGAGCAGGCCGAACACA‑ACCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACACGGCTGGa                                                                                                                 >  4:117356/1‑149 (MQ=255)                         aaGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGa                                                                                                                 <  2:104189/149‑1 (MQ=255)                          aGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGAc                                                                                                                <  6:27832/149‑1 (MQ=255)                             gCTACCCCGAGCATGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGTCTGGACCTg                                                                                                             <  8:47007/149‑1 (MQ=255)                             gCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTg                                                                                                             <  2:120101/149‑1 (MQ=255)                                 ccccGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTAc                                                                                                          <  7:2929/148‑1 (MQ=255)                                    cGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAcc                                                                                                      <  2:67334/149‑1 (MQ=255)                                      aGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAcccc                                                                                                    <  8:127985/149‑1 (MQ=255)                                         aGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTg                                                                                                 <  7:166957/149‑1 (MQ=255)                                           gCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTTCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGCCTGGACCTGTACGACCCCGTgcg                                                                                               >  7:195406/1‑149 (MQ=255)                                           gCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCGCCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCg                                                                                                   <  1:265571/145‑1 (MQ=255)                                           gCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCGCCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCg                                                                                                   >  2:265571/1‑145 (MQ=255)                                            ccGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTgcgc                                                                                              <  3:140689/149‑1 (MQ=255)                                             cGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTCTACGACCCCGTGCGCt                                                                                             >  3:233578/1‑149 (MQ=255)                                                  aca‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGa                                                                                                                                 <  7:136372/108‑1 (MQ=255)                                                  aca‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGa                                                                                                                                 >  8:136372/1‑108 (MQ=255)                                                  aca‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATc                                                                                        >  6:210481/1‑149 (MQ=255)                                                   ca‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATcc                                                                                       <  2:44391/149‑1 (MQ=255)                                                    a‑aaCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCg                                                                                      >  5:263666/1‑149 (MQ=255)                                                    a‑aaCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCg                                                                                      >  1:277945/1‑149 (MQ=255)                                                       aCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGca                                                                                    <  5:332650/149‑1 (MQ=255)                                                       aCGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGca                                                                                    >  6:235798/1‑149 (MQ=255)                                                          ccAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacc                                                                                  >  2:107803/1‑148 (MQ=255)                                                            acgAGCTGAGCACCGCCCGGCTGCACGCCAGCCGCGGCGGCCTGGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTTCGCTTCATCCGcacccac                                                                               <  4:233578/147‑1 (MQ=255)                                                            aGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                               <  7:110506/149‑1 (MQ=255)                                                             ggAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccacc                                                                              >  7:131695/1‑149 (MQ=255)                                                             ggAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccacc                                                                              >  6:243860/1‑149 (MQ=255)                                                              gAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCt                                                                             <  1:18163/149‑1 (MQ=255)                                                                 cTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAcccc                                                                                                    <  8:34244/123‑1 (MQ=255)                                                                 cTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGAcccc                                                                                                    >  7:34244/1‑123 (MQ=255)                                                                   gATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCa                                                                                          <  7:41891/131‑1 (MQ=255)                                                                   gATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCa                                                                                          >  8:41891/1‑131 (MQ=255)                                                                      cACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGc                                                                                                                     >  6:138905/1‑101 (MQ=255)                                                                      cACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGc                                                                                                                     <  5:138905/101‑1 (MQ=255)                                                                         cGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTcgaccgac                                                                  <  2:9403/149‑1 (MQ=255)                                                                          gCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACt                                                                 <  3:117356/149‑1 (MQ=255)                                                                           cccTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                               >  1:266324/1‑134 (MQ=255)                                                                           cccTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGcacccac                                                                               <  2:266324/134‑1 (MQ=255)                                                                             ctgctgCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGa                                                              <  1:200437/149‑1 (MQ=255)                                                                             ctgctgCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGa                                                              <  2:239199/149‑1 (MQ=255)                                                                                    aCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCt                                                                             >  8:318012/1‑127 (MQ=255)                                                                                    aCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCt                                                                             <  7:318012/127‑1 (MQ=255)                                                                                    aCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAcc                                                       >  8:82533/1‑149 (MQ=255)                                                                                    aCGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAcc                                                       <  6:142738/149‑1 (MQ=255)                                                                                      gCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCAcccc                                                     >  2:55454/1‑149 (MQ=255)                                                                                          gCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAg                                                 <  2:277945/149‑1 (MQ=255)                                                                                           ccGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAgg                                                <  8:49731/149‑1 (MQ=255)                                                                                            cgcgGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGa                                               <  3:244626/149‑1 (MQ=255)                                                                                             gcggcgGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGAc                                              >  6:135677/1‑149 (MQ=255)                                                                                              cggcggCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTg                                                                <  7:175226/130‑1 (MQ=255)                                                                                              cggcggCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTg                                                                >  8:175226/1‑130 (MQ=255)                                                                                                gcggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGaa                                           <  5:135677/149‑1 (MQ=255)                                                                                                 cggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAAc                                          <  6:263666/149‑1 (MQ=255)                                                                                                  ggcCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAAcc                                         <  7:82533/149‑1 (MQ=255)                                                                                                           ccGATCTATTGCGACACAAGCCCCTGCACCCTGCGCT‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgt                                <  5:328024/149‑1 (MQ=255)                                                                                                             gATCTATTGCGACACAAGCCCCTGCACCCTGCGCCTTGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgtg                               >  1:52010/1‑149 (MQ=255)                                                                                                              aTCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCa                             >  1:7610/1‑149 (MQ=255)                                                                                                                 tATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGt                          >  1:52898/1‑149 (MQ=255)                                                                                                                   ttGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCtgtg                               <  5:235798/142‑1 (MQ=255)                                                                                                                      cGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGACCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGAcc                      <  2:320721/148‑1 (MQ=255)                                                                                                                          acaAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGCCCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTgcag                 >  7:321275/1‑149 (MQ=255)                                                                                                                           tcaggtccTGCACCAGGCGCA‑GGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACAGGTAGCTGTGCACGTGACCTgcag                 >  2:179793/7‑148 (MQ=255)                                                                                                                            aaGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTa                                                                                                           >  2:114134/1‑57 (MQ=255)                                                                                                                            aaGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTa                                                                                                           <  1:114134/57‑1 (MQ=255)                                                                                                                              gCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGcagcacc             <  2:52898/149‑1 (MQ=255)                                                                                                                              gCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACACCCCAGGACGAACCGGAAGCTGTGCCCGTGACCTGcagcacc             >  6:4249/1‑149 (MQ=255)                                                                                                                                         cTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTggg  <  8:131695/149‑1 (MQ=255)                                                                                                                                         cTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTggg  >  5:87219/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                              TGCTGCGGCCAACCGTTCGCCTCCAAGGGCTACCCCGAGCAGGCCGAACACA‑AACGCCAGGAGCTGATCACCGCCCTGCTGCACGCCAGCCGCGGCGGCCTCGACCCGATCTATTGCGACACAAGCCCCTGCACCCTGCGCC‑TGGTGCAGGACCTGAGCGACACCCGGCTGGACCTGTACGACCCCGTGCGCTTCATCCGCACCCACCTGCTCGACCGACTGGAGTTCACCCCCCAGGACGAACCGGTAGCTGTGCACGTGACCTGCAGCACCCAGCACCTGGG  >  NC_002947/5388741‑5389024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑