Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,396,355 T→A 8.5% F278Y (TTC→TAC)  hsdM → type I restriction modification system methylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,396,3550TA 8.5% 165.5 / 5.3 59F278Y (TTC→TAC) hsdMtype I restriction modification system methylase
Reads supporting (aligned to +/- strand):  ref base T (26/28);  new base A (2/3);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

CCGCACCTGCTGGCCACCACCAACATGATCCTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCAGTGGTACTGCCCGATGGCTTCCTTTT  >  NC_002947/5396213‑5396502
                                                                                                                                              |                                                                                                                                                   
ccGCACCTGCTGGCCACCACCAACATGATCCTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTcggc                                                                                                                                                 <  4:241127/147‑1 (MQ=255)
     ccTGCTGGCCACCACCAACATGATCCTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATg                                                                                                                                           >  4:113145/1‑148 (MQ=255)
        gctgGCCACCACCAACATGATCCTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATgg                                                                                                                                          >  6:85429/1‑146 (MQ=255)
         ctgGCCACCACCAACATGATCCTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGgaa                                                                                                                                        <  7:313149/147‑1 (MQ=255)
         ctgGCCACCACCAACATGATCCTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGgaa                                                                                                                                        <  6:79602/147‑1 (MQ=255)
                 caccaACATGATCCTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTa                                                                                                                              >  6:72824/1‑149 (MQ=255)
                  accaACATGATCCTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTAt                                                                                                                             <  3:113145/149‑1 (MQ=255)
                        aTGATCCTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTACGGCGGCATGGAAGAAGACGGTATCGAAAc                                                                                                                       <  1:29619/149‑1 (MQ=255)
                             ccTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAAtt                                                                                                                  >  2:39373/1‑149 (MQ=255)
                              cTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAAttt                                                                                                                 >  4:181735/1‑149 (MQ=255)
                              cTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAAttt                                                                                                                 >  4:177109/1‑149 (MQ=255)
                              atGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCGTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCATCCCGCCGTTCGGCGGCAt                                                                                                                                            <  2:142905/121‑1 (MQ=255)
                                gCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCg                                                                                                                           >  8:228703/1‑137 (MQ=255)
                                gCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCg                                                                                                                           <  7:228703/137‑1 (MQ=255)
                                   tGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTc                                                                                                               <  8:326028/146‑1 (MQ=255)
                                   tGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTc                                                                                                               >  7:326028/1‑146 (MQ=255)
                                       aTCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGAAcggcgggttg                                                                                                                                           <  7:290075/114‑5 (MQ=255)
                                       aTCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGAAcggcgggttg                                                                                                                                           >  8:290075/1‑110 (MQ=255)
                                                  cagccagATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCAccc                                                                                             >  5:81603/1‑149 (MQ=255)
                                                  cagccagATCCGGCACGACAACACCCTGAGCAAACCGCTGATCTGCTGGGGCCCACGCGAGCGGGGGCATTGTTTCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTTCCGCTTTCCGCAccc                                                                                             <  2:253765/149‑1 (MQ=255)
                                                            cGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAAccgc                                                                                   <  7:131039/149‑1 (MQ=255)
                                                             gTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAAccgcc                                                                                  >  5:208418/1‑149 (MQ=255)
                                                                aCGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGa                                                                                <  5:254978/148‑1 (MQ=255)
                                                                    caacaCCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGt                                                                           <  6:81603/149‑1 (MQ=255)
                                                                     aacaCCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGtt                                                                          <  5:85429/149‑1 (MQ=255)
                                                                     aacaCCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGtt                                                                          >  4:130472/1‑149 (MQ=255)
                                                                        acCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCtt                                                                       >  2:229253/1‑149 (MQ=255)
                                                                              aGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGCTAt                                                                  <  3:181735/148‑1 (MQ=255)
                                                                              aGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGCTAt                                                                  <  3:177109/148‑1 (MQ=255)
                                                                                cAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCt                                                                        >  1:256151/1‑140 (MQ=255)
                                                                                cAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCt                                                                        <  2:256151/140‑1 (MQ=255)
                                                                                 aaaCCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGAt                                                              <  5:72824/149‑1 (MQ=255)
                                                                                  aaCCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCTCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGAtt                                                             <  3:11413/149‑1 (MQ=255)
                                                                                   aCCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTg                                                                      >  2:87882/1‑139 (MQ=255)
                                                                                   aCCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTg                                                                      <  1:87882/139‑1 (MQ=255)
                                                                                   aCCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTAtt                                                                 >  2:91936/1‑144 (MQ=255)
                                                                                   aCCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTAtt                                                                 <  1:91936/144‑1 (MQ=255)
                                                                                     cGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATg                                                          >  7:96453/1‑149 (MQ=255)
                                                                                      gCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAAccgcc                                                                                  <  2:12065/124‑1 (MQ=255)
                                                                                      gCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACACGGGAAAccgcc                                                                                  >  1:12065/1‑124 (MQ=255)
                                                                                              gCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCTCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCATCTGGTc                                                 <  6:208418/149‑1 (MQ=255)
                                                                                              gCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGc                                                         >  1:159306/1‑141 (MQ=255)
                                                                                              gCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGc                                                         <  2:159306/141‑1 (MQ=255)
                                                                                                tGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCa                                                >  1:10977/1‑148 (MQ=255)
                                                                                                  gggCCCAAGCGAGCGCGTGCATTGTATCGGCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAg                                             <  6:85092/149‑1 (MQ=255)
                                                                                                       cAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGAtggt                                        >  8:195477/1‑149 (MQ=255)
                                                                                                       cAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGAtggt                                        >  3:176969/1‑149 (MQ=255)
                                                                                                                  gTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGgccgcgccg                              >  7:123822/1‑148 (MQ=255)
                                                                                                                   tGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCa                            <  8:96453/149‑1 (MQ=255)
                                                                                                                      aTTGGATCGGCGCCAACCCGCAGTTCGTCTGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATTGTGGCCGCGCCGCAGTg                         <  8:153609/149‑1 (MQ=255)
                                                                                                                          tATCGTCCCCAACCCGCCGTTCGTCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCAGTGGTa                      <  7:233725/148‑1 (MQ=255)
                                                                                                                            tcgtcgCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCAGTGGTACTg                   >  8:276386/1‑149 (MQ=255)
                                                                                                                               tcgCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCAGTGGTACTGccc                >  7:250323/1‑149 (MQ=255)
                                                                                                                                 gCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCAGTGGTACTGCCCGa              <  1:39373/149‑1 (MQ=255)
                                                                                                                                  ccatgccgccgAACGGCGGGTTGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCAGTGGTACTGc                  >  8:225007/6‑144 (MQ=255)
                                                                                                                                  ccatgccgccgAACGGCGGGTTGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCAGTGGTACTGc                  <  7:225007/139‑1 (MQ=255)
                                                                                                                                      cccGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCAGTGGTACTGCCCGATGGCt         >  2:206328/1‑149 (MQ=255)
                                                                                                                                         gccgTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCAGTGGTACTGCCCGATGGc          >  3:89299/1‑145 (MQ=255)
                                                                                                                                         gccgTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCAGTGGTACTGCCCGATGGc          <  4:89299/145‑1 (MQ=255)
                                                                                                                                             ttCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCAGTGGTACTGCCCGATGGCTTCCtttt  <  7:276386/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CCGCACCTGCTGGCCACCACCAACATGATCCTGCATGGCATCGAAGTGCCCAGCCAGATCCGTCACGACAACACCCTGAGCAAACCGCTGATCAGCTGGGGCCCAAGCGAGCGCGTGCATTGTATCGTCGCCAACCCGCCGTTCGGCGGCATGGAAGAAGACGGTATCGAAACAAATTTTCCTGCCGCTTTCCGCACCCGGGAAACCGCCGATTTGTTCTTGGTATTGATTATGCAGCTGCTCAAAGATGGTGGCCGCGCCGCAGTGGTACTGCCCGATGGCTTCCTTTT  >  NC_002947/5396213‑5396502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑