Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,407,046 T→G 7.1% N124T (AAC→ACC)  PP_4751 ← amino acid ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,407,0460TG 7.1% 215.4 / 6.6 70N124T (AAC→ACC) PP_4751amino acid ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (31/34);  new base G (3/2);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

CGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCG  >  NC_002947/5406905‑5407193
                                                                                                                                             |                                                                                                                                                   
cGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCgca                                                                                                                                               <  2:159163/148‑1 (MQ=255)
   tgctgcTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTgctgcgcaa                                                                                                                                           >  5:280421/1‑142 (MQ=255)
     ctgctgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGTGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCg                                                                                                                                         >  4:331409/1‑149 (MQ=255)
     ctgctgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCAcc                                                                                                                                          <  1:323454/148‑1 (MQ=255)
         tgGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAg                                                                                                                                     >  1:266034/1‑149 (MQ=255)
           gCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGt                                                                                                                                   >  2:309554/1‑149 (MQ=255)
              accaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAgcctgg                                                                                                                                <  6:280421/149‑6 (MQ=255)
               ccaGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                               <  7:46262/149‑1 (MQ=255)
                caGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCgg                                                                                                                               >  7:197770/1‑148 (MQ=255)
                  gagCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGaa                                                                                                                            >  3:326943/1‑149 (MQ=255)
                    gcagcGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGACTGACGTTGCGCAGCACCGACAGGTGCGGGac                                                                                                                            >  6:234686/1‑146 (MQ=255)
                      agcGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAgg                                                                                                                        <  1:73269/149‑1 (MQ=255)
                       gcgcgcCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGt                                                                                                                       <  7:20652/149‑1 (MQ=255)
                            ccGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAAc                                                                                                                  >  5:80273/1‑149 (MQ=255)
                             cGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCATGCTGACGTTGCTCAGCACCGTCAAGTGCGGGAACAGGTTGAAc                                                                                                                  >  6:270995/1‑148 (MQ=255)
                               gATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGTTGCGCATTTGCGCCAGGCTGACGTTGCGCAGCACCTACAGGGGCGGGAACAGGTTAAACGaa                                                                                                               >  4:326055/1‑148 (MQ=255)
                                      cTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCACGTGCGGGTACAGGTTGAACGACTGGAaca                                                                                                        >  7:22531/1‑149 (MQ=255)
                                         ggCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGTCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAacac                                                                                                       <  7:80988/147‑1 (MQ=255)
                                         ggCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAacac                                                                                                       <  8:197770/147‑1 (MQ=255)
                                         ggCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCa                                                                                                     >  8:27398/1‑149 (MQ=255)
                                         ggCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCa                                                                                                     >  3:248279/1‑149 (MQ=255)
                                           ccTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACAcc                                                                                                      <  5:28445/146‑1 (MQ=255)
                                           ccTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACAcc                                                                                                      >  6:28445/1‑146 (MQ=255)
                                           ccTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATa                                                                                                   >  5:166591/1‑149 (MQ=255)
                                           ccTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATa                                                                                                   >  2:333232/1‑149 (MQ=255)
                                           ccTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGCACCACCATa                                                                                                   >  3:211263/1‑149 (MQ=255)
                                              tGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCCGGAACAGGTTGAACGACAGGAACACCATAcc                                                                                                 >  4:293617/1‑148 (MQ=255)
                                               gTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCa                                                                                                     >  3:235723/1‑143 (MQ=255)
                                               gTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCa                                                                                                     <  4:235723/143‑1 (MQ=255)
                                                tCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGTGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCaa                                                                                               <  4:59897/148‑1 (MQ=255)
                                                tCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAAt                                                                                              <  2:266034/149‑1 (MQ=255)
                                                tCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAAt                                                                                              <  5:270995/149‑1 (MQ=255)
                                                  agccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGTGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATgc                                                                                            <  3:331409/149‑1 (MQ=255)
                                                  agccagccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATgc                                                                                            <  2:68452/149‑1 (MQ=255)
                                                      agccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAAc                                                                                                                  >  4:237039/1‑123 (MQ=255)
                                                      agccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAAc                                                                                                                  <  3:237039/123‑1 (MQ=255)
                                                      agccCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCg                                                                                        >  5:317811/1‑149 (MQ=255)
                                                            aCGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAac                                                                                                         >  1:54464/1‑126 (MQ=255)
                                                            aCGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAac                                                                                                         <  2:54464/126‑1 (MQ=255)
                                                               cgTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACtt                                                                               >  6:43739/1‑149 (MQ=255)
                                                                gTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTc                                                                              <  6:317811/149‑1 (MQ=255)
                                                                    gAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTcagc                                                                           <  8:148634/148‑1 (MQ=255)
                                                                    gAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTcag                                                                            >  6:127529/1‑147 (MQ=255)
                                                                        aGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACGGTCAGGTGCGGTAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTc                                                                      <  3:293617/149‑1 (MQ=255)
                                                                         gTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCt                                                                     <  7:4482/149‑1 (MQ=255)
                                                                         gTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCt                                                                     <  6:80273/149‑1 (MQ=255)
                                                                          ttGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAgcc                                                                                                                                   <  1:161579/86‑3 (MQ=37)
                                                                          ttGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGGTGCTGCGCAACGTCAgcc                                                                                                                                   >  2:161579/1‑84 (MQ=37)
                                                                            gCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGt                                                                   <  5:43739/148‑1 (MQ=255)
                                                                                     gggCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAAc                                                         <  2:178742/149‑1 (MQ=255)
                                                                                       gCGTCGGCTTCTTTGGAGCTGCGGCCCAAGGTGCGGAGTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACCCCTTACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGc                                                       <  3:252122/149‑1 (MQ=255)
                                                                                            ggCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTg                                                   <  6:166591/148‑1 (MQ=255)
                                                                                            ggCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACAGTCAGGTGCGGGAACAGTTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTAAGGGGCAACCCCTTg                                                   >  7:286051/1‑148 (MQ=255)
                                                                                             gCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGc                                                  >  1:124735/1‑148 (MQ=255)
                                                                                             gCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGc                                                  <  2:124735/148‑1 (MQ=255)
                                                                                             gCTTCGTTGGCGCTGGGGGCCAAGGGGCGGTTTTGCGCCATGCTTAAGTTGCGCATCACCGTGATGTGCGGGAACAGGGTTAACGACTTGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGcc                                                 <  4:211263/149‑1 (MQ=255)
                                                                                               ttcttTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAg                                               >  7:268377/1‑149 (MQ=255)
                                                                                                    tGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGc                                                  >  7:153022/1‑141 (MQ=255)
                                                                                                    tGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGc                                                  <  8:153022/141‑1 (MQ=255)
                                                                                                    tGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCtt                                            <  4:248279/147‑1 (MQ=255)
                                                                                                      gcgcTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGAt                                        <  1:309554/149‑1 (MQ=255)
                                                                                                        gcTGCGGCCCAAGGTGCGGATTTGCGCCAGACTGACGTTGCGCAGCACCGTCAGGTGCGGGTACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGAtgt                                      <  5:234686/149‑1 (MQ=255)
                                                                                                          tGCGGCCCAAGGTGCGGATTTGCGCCAGGCGTACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGc                                    <  4:326943/149‑1 (MQ=255)
                                                                                                                ccAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGcca                              <  1:333232/149‑1 (MQ=255)
                                                                                                                 cAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGccat                             >  2:62159/1‑149 (MQ=255)
                                                                                                                          gATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGa                    >  6:279021/1‑149 (MQ=255)
                                                                                                                                cgcCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGc               <  5:127529/148‑1 (MQ=255)
                                                                                                                                      gCTGACGTTGCGCAGCACCGTCAGGTGCGGGACCAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTgg        >  2:70511/1‑149 (MQ=255)
                                                                                                                                       cTGACGGTGCTGCGCAACGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCCACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGt       >  7:94263/1‑149 (MQ=255)
                                                                                                                                            gTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTcggcg  >  3:91415/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                   
CGTTGCTGCTGGCCACCAGAGCAGCGCGCCGGATAATGCTGGGCCTTGTCAGCCAGCCCAACGCGTTCGAGCAGTTGCAAGGAGCGGGCGTCGGCTTCTTTGGCGCTGCGGCCCAAGGTGCGGATTTGCGCCAGGCTGACGTTGCGCAGCACCGTCAGGTGCGGGAACAGGTTGAACGACTGGAACACCATACCAATGCGCCGGCGCAACTTCAGCAGTTCTGTACGGGGCAACGCCTTGCCAGCTTCGATGTGCACGCCATCCATGCTGACCCGCCCCTTGGTCGGCG  >  NC_002947/5406905‑5407193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑