Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 71,796 C→G 10.1% A37P (GCT→CCT)  glyS ← glycine‑tRNA ligase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294771,7960CG10.1% 201.8 / 12.6 69A37P (GCT→CCT) glySglycine‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (34/28);  new base G (5/2);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 6.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-01

CCTTCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTT  >  NC_002947/71651‑71944
                                                                                                                                                 |                                                                                                                                                    
ccTTCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGTTGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTg                                                                                                                                                   >  1:244808/1‑149 (MQ=255)
 cTTCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGc                                                                                                                                                  <  4:109864/149‑1 (MQ=255)
  ttCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTg                                                                                                                                                   >  8:304937/1‑147 (MQ=255)
            aaGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCttttt                                                                                                                                        <  7:304937/148‑1 (MQ=255)
               gCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCTGCAGGCTGCTGGCCt                                                                                                                                            <  4:203101/141‑1 (MQ=255)
               gCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCTGCAGGCTGCTGGCCt                                                                                                                                            >  3:203101/1‑141 (MQ=255)
                      gCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGc                                                                                                                              >  4:280568/1‑148 (MQ=255)
                         ggggCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAg                                                                                                                           >  8:28812/1‑148 (MQ=255)
                          gggcgggcCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAgg                                                                                                                          >  5:283228/1‑148 (MQ=255)
                           ggcgggcCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGaa                                                                                                                        <  7:216321/149‑1 (MQ=255)
                               ggcCGTCGATGTAGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGCCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCg                                                                                                                    >  1:112195/1‑149 (MQ=255)
                                   gTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGc                                                                                                                >  8:229166/1‑149 (MQ=255)
                                          ttGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCagcctt                                                                                                         >  3:30986/1‑144 (MQ=255)
                                           tGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTg                                                                                                        >  1:1911/1‑149 (MQ=255)
                                                ctgcGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTg                                                                                                                                                   <  1:216595/101‑1 (MQ=255)
                                                ctgcGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTg                                                                                                                                                   >  2:216595/1‑101 (MQ=255)
                                                 tgcGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGTCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCATCCTGCAGGCCTTTTTCGATAACGTCCAGGAAGGCGTCTCCAAGGCTGGCGAgg                                                                                                  <  1:320641/149‑1 (MQ=255)
                                                  gcGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTGTTTCGATat                                                                                                                                  >  4:167952/1‑115 (MQ=255)
                                                   cGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAggg                                                                                                 <  6:283228/148‑1 (MQ=255)
                                                       cAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTg                                                                                            >  7:111054/1‑149 (MQ=255)
                                                          gTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTgg                                                                                           >  7:312763/1‑147 (MQ=255)
                                                          gTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGt                                                                                         <  1:289490/149‑1 (MQ=255)
                                                          gTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGt                                                                                         <  2:112195/149‑1 (MQ=255)
                                                             gctgcACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTCTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCACGGCTGGCGAGGGCCTTGGGTgg                                                                                       >  7:301710/1‑148 (MQ=255)
                                                                gcACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCTGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGc                                                                                                                <  8:257186/120‑1 (MQ=255)
                                                                gcACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCTGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGc                                                                                                                >  7:257186/1‑120 (MQ=255)
                                                                gcACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGc                                                                                   >  7:65731/1‑149 (MQ=255)
                                                                 cACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGc                                                                                   <  7:23976/148‑1 (MQ=255)
                                                                     tCCAGCTGGCGACTCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTc                                                                              <  2:1911/149‑1 (MQ=255)
                                                                       cAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCg                                                                             <  8:111054/148‑1 (MQ=255)
                                                                        aGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGt                                                                           >  5:51400/1‑149 (MQ=255)
                                                                        aGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGt                                                                           >  5:27377/1‑149 (MQ=255)
                                                                        aGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGt                                                                           <  2:244808/149‑1 (MQ=255)
                                                                             gCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGAGGCAGCTCTTCGGTGccc                                                                       <  8:312763/148‑1 (MQ=255)
                                                                              cGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAg                                                                     >  7:238893/1‑149 (MQ=255)
                                                                               gAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGt                                                                    >  4:211033/1‑149 (MQ=255)
                                                                                aaTCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGcc                                                                        >  6:81497/1‑144 (MQ=255)
                                                                                aaTCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGcc                                                                        <  5:81497/144‑1 (MQ=255)
                                                                                  tCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTc                                                                  >  4:294637/1‑148 (MQ=255)
                                                                                    aGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTAc                                                               <  6:27377/149‑1 (MQ=255)
                                                                                    aGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTAc                                                               <  7:229166/149‑1 (MQ=255)
                                                                                              gacgacGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCt                                                     <  3:280568/149‑1 (MQ=255)
                                                                                               acgacgCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCtt                                                    >  8:150343/1‑149 (MQ=255)
                                                                                                    gcggcgcggcGTACACCTGCTTGCGGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCa                                               >  5:196917/1‑149 (MQ=255)
                                                                                                        cgcggcgTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCa                                           <  7:28812/149‑1 (MQ=255)
                                                                                                         gcggcgTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCAt                                          >  6:236967/1‑149 (MQ=255)
                                                                                                                acacCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAg                                   >  7:79532/1‑149 (MQ=255)
                                                                                                                 cacCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGc                                  <  5:236967/149‑1 (MQ=255)
                                                                                                                 cacCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGc                                  <  8:238893/149‑1 (MQ=255)
                                                                                                                 cacCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGc                                  <  3:294637/149‑1 (MQ=255)
                                                                                                                  acCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTgg                                                                                           >  6:100919/1‑91 (MQ=255)
                                                                                                                  acCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTgg                                                                                           <  5:100919/91‑1 (MQ=255)
                                                                                                                  acCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGcc                                 >  5:191425/1‑149 (MQ=255)
                                                                                                                    cTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTc                               >  6:276921/1‑149 (MQ=255)
                                                                                                                      gCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGa                                                  >  3:212697/1‑128 (MQ=255)
                                                                                                                      gCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGa                                                  <  4:212697/128‑1 (MQ=255)
                                                                                                                        ttGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGc                           >  4:258158/1‑149 (MQ=255)
                                                                                                                          gCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGc                           <  3:211033/147‑1 (MQ=255)
                                                                                                                           ccGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTa                        <  6:119969/149‑1 (MQ=255)
                                                                                                                                 tAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCaa                   >  5:163964/1‑148 (MQ=255)
                                                                                                                                 tAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAac                  <  8:79532/149‑1 (MQ=255)
                                                                                                                                  gaaaaaGGCCTGCAGGCTGCTGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCAtt                                         <  4:48989/120‑1 (MQ=255)
                                                                                                                                  gaaaaaGGCCTGCAGGCTGCTGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAaca                 >  1:44653/6‑149 (MQ=255)
                                                                                                                                  gaaaaaGGCCTGCAGGCTGCTGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCGTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCAtt                                         >  3:48989/6‑125 (MQ=255)
                                                                                                                                   aaaaaGGCCTGCAGGCTGCTGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAacac                >  4:16572/5‑149 (MQ=255)
                                                                                                                                    ttCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCAtt                                         >  2:99630/1‑123 (MQ=255)
                                                                                                                                    ttCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCAtt                                         <  1:99630/123‑1 (MQ=255)
                                                                                                                                    ttCAGGACAGCAGCCTGCAGGCCTTTTTCGATCCCGGCCAGGAAGGCGTCGCCAACGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTAACAGGAAATCTTGAGCGCTCATTGTGCAGCCTCAAGCTTAGACAaccc                >  2:251609/1‑146 (MQ=255)
                                                                                                                                        ggCCTGCAGGCTGCTGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGt           >  4:259213/1‑149 (MQ=255)
                                                                                                                                             gcagcCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCtt                                                    >  6:291614/1‑103 (MQ=255)
                                                                                                                                             gcagcCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCtt                                                    <  5:291614/103‑1 (MQ=255)
                                                                                                                                              cagcCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACg       >  7:235/1‑147 (MQ=255)
                                                                                                                                                 ccTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGtt  <  5:276921/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CCTTCGGCGTTGAAGGCAGCCTGCAGGGGCGGGCCGTCGATGTTGATGCTGCGGTCAGGTTGCTGCACGTCCAGCTGGCGAATCAGCACGGCCAGACGACGCGGCGCGGCGTACACCTGCTTGCCGGTGTAGTTCAGGCCAGCAGCCTGCAGGCCTTTTTCGATACCGGCCAGGAAGGCGTCGCCAAGGCTGGCGAGGGCCTTGGGTGGCAGCTCTTCGGTGCCCAGTTCTACCAGGAAATCTTGAGCACTCATTGTGCAGCCTCCAGCTTAGCCAACACTTCGTCACGCAGTT  >  NC_002947/71651‑71944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑