Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,427,005 G→A 8.1% T366T (ACC→ACT) ‡ rhlE‑I ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,427,0050GA 8.1% 170.8 / 3.9 62T366T (ACC→ACT) ‡rhlE‑IATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base G (26/30);  new base A (2/3);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01

TGCTGTCCATCCAACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCGGCAGGTCGAG  >  NC_002947/5426857‑5427148
                                                                                                                                                    |                                                                                                                                               
tGCTGTCCATCCAACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAgg                                                                                                                                                 >  1:79109/1‑149 (MQ=255)
 gCTGTCCATCCAACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAggg                                                                                                                                                >  4:258303/1‑149 (MQ=255)
     tccatccaACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTg                                                                                                                                            >  1:91460/1‑149 (MQ=255)
       catccaACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGcc                                                                                                                                          >  3:63699/1‑149 (MQ=255)
           caACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGt                                                                                                                                      >  5:210677/1‑149 (MQ=255)
            aaCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGt                                                                                                                                      >  2:286731/1‑148 (MQ=255)
            aaCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgc                                                                                                                                        <  3:328000/146‑2 (MQ=255)
            aaCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgc                                                                                                                                        >  4:328000/1‑145 (MQ=255)
             aCGCGCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCa                                                                                                                                    <  5:156467/149‑1 (MQ=255)
             aCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCa                                                                                                                                    <  8:70171/149‑1 (MQ=255)
             aCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgcgcca                                                                                                                                    <  7:193685/149‑6 (MQ=255)
              cGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGTGTCTGCCGGGTCAg                                                                                                                                   <  8:28079/149‑1 (MQ=255)
               gCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAg                                                                                                                                   >  1:281193/1‑148 (MQ=255)
                   cAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACtt                                                                                                                              <  6:230947/149‑1 (MQ=255)
                    aGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAATCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACtt                                                                                                                              >  2:91737/1‑148 (MQ=255)
                       ccGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAAt                                                                                                                          <  4:63699/149‑1 (MQ=255)
                           gcTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCTTCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGgcc                                                                                                                      <  2:270585/149‑1 (MQ=255)
                               ggCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGgccgc                                                                                                                    <  3:212948/147‑1 (MQ=255)
                               ggCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGgccgc                                                                                                                    <  2:281193/147‑1 (MQ=255)
                                      ttttCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGtt                                                                                                            <  1:286731/148‑1 (MQ=255)
                                      ttttCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGtt                                                                                                            <  2:91460/148‑1 (MQ=255)
                                           tttcGGCTTCTTGGGCTTCTTCGGTGTCTTGATAACCTGCCCACTGGCATCGGTCATAGGCACCCGGTGCTAGGGGATGAAATCAGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCACCAGTTGCACt                                                                                                       >  1:48089/1‑148 (MQ=255)
                                             tcGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgcgcca                                                                                                                                    <  2:12049/117‑6 (MQ=255)
                                             tcGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACATGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgcgcca                                                                                                                                    >  1:12049/1‑112 (MQ=255)
                                               ggCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCca                                                                                                                  >  4:80446/1‑133 (MQ=255)
                                               ggCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCca                                                                                                                  <  3:80446/133‑1 (MQ=255)
                                               ggCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCAt                                                                                                   <  1:10700/148‑1 (MQ=255)
                                                          cttcttCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCacacac                                                                                         <  2:66798/147‑1 (MQ=255)
                                                          cttcttCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCCGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCacacac                                                                                         >  1:66798/1‑147 (MQ=255)
                                                             cttcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCCCTTCATCCGCACACACCAGc                                                                                     >  8:49567/1‑148 (MQ=255)
                                                              ttcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGc                                                                                               >  2:27889/1‑137 (MQ=255)
                                                              ttcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGc                                                                                               <  1:27889/137‑1 (MQ=255)
                                                               tcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGcacac                                                                                           <  8:295642/140‑1 (MQ=255)
                                                               tcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGcacac                                                                                           >  7:295642/1‑140 (MQ=255)
                                                               tcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGca                                                                                              <  2:77262/137‑1 (MQ=255)
                                                               tcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGATGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGca                                                                                              >  1:77262/1‑137 (MQ=255)
                                                                  gTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAAtc                                                                               <  3:258303/149‑1 (MQ=255)
                                                                   tttCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAAtcg                                                                              >  3:132629/1‑149 (MQ=255)
                                                                         gATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAAtcgcctcgc                                                                        >  6:54664/1‑149 (MQ=255)
                                                                              ccTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCttg                                                                   >  1:343259/1‑149 (MQ=255)
                                                                                  cccACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCttgtt                                                                 <  2:74572/147‑1 (MQ=255)
                                                                                         gCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTgcccgcccg                                                        <  5:46497/149‑1 (MQ=255)
                                                                                          cATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTgcccgcccgc                                                       >  2:237024/1‑149 (MQ=255)
                                                                                           aTCGTTCATCGGCACCCGGCGCTCGGGGATGAAATCCGGCTGTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCttgtt                                                                 <  6:107866/138‑1 (MQ=255)
                                                                                           aTCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCATTGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGATgcccgcccgcc                                                      >  2:122427/1‑149 (MQ=255)
                                                                                           aTCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTgcccgcccgcc                                                      <  1:91737/149‑1 (MQ=255)
                                                                                            tCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTgcccgcccgccc                                                     >  5:270590/1‑149 (MQ=255)
                                                                                               gTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCgg                                                   >  8:285073/1‑148 (MQ=255)
                                                                                                                   ggggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGGCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACg                               <  2:343259/148‑1 (MQ=255)
                                                                                                                    gggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACg                               >  6:201115/1‑147 (MQ=255)
                                                                                                                    gggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTa                             <  4:132629/149‑1 (MQ=255)
                                                                                                                    gggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTa                             <  2:79109/149‑1 (MQ=255)
                                                                                                                     ggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGAt                                         >  4:4784/1‑136 (MQ=255)
                                                                                                                     ggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGAt                                         <  3:4784/136‑1 (MQ=255)
                                                                                                                     ggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAg                            <  1:122427/149‑1 (MQ=255)
                                                                                                                               cGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCaa                  <  5:54664/149‑1 (MQ=255)
                                                                                                                                ggCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGc                                       >  4:141784/1‑127 (MQ=255)
                                                                                                                                ggCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGc                                       <  3:141784/127‑1 (MQ=255)
                                                                                                                                   tctTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCg                      <  5:273213/141‑1 (MQ=255)
                                                                                                                                   tctTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCg                      >  6:273213/1‑141 (MQ=255)
                                                                                                                                      tCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACATAGTCTTCGGCAACGATCgg           >  1:173315/1‑149 (MQ=255)
                                                                                                                                        aTGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCgg           >  1:222629/1‑147 (MQ=255)
                                                                                                                                               ggCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCGGCAGGTCGAg  <  1:237024/149‑1 (MQ=255)
                                                                                                                                               ggCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCGGCAGGTCGAg  <  2:222629/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TGCTGTCCATCCAACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCGGCAGGTCGAG  >  NC_002947/5426857‑5427148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: