Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,427,006 G→C 8.0% T366S (ACC→AGC) ‡ rhlE‑I ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,427,0060GC 8.0% 188.3 / 5.4 62T366S (ACC→AGC) ‡rhlE‑IATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base G (26/31);  new base C (2/3);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

GCTGTCCATCCAACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCGGCAGGTCGAG  >  NC_002947/5426858‑5427148
                                                                                                                                                    |                                                                                                                                              
gCTGTCCATCCAACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAggg                                                                                                                                                >  4:258303/1‑149 (MQ=255)
    tccatccaACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTg                                                                                                                                            >  1:91460/1‑149 (MQ=255)
      catccaACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGcc                                                                                                                                          >  3:63699/1‑149 (MQ=255)
          caACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGt                                                                                                                                      >  5:210677/1‑149 (MQ=255)
           aaCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGt                                                                                                                                      >  2:286731/1‑148 (MQ=255)
           aaCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgc                                                                                                                                        >  4:328000/1‑145 (MQ=255)
           aaCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgc                                                                                                                                        <  3:328000/146‑2 (MQ=255)
            aCGCGCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCa                                                                                                                                    <  5:156467/149‑1 (MQ=255)
            aCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCa                                                                                                                                    <  8:70171/149‑1 (MQ=255)
            aCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgcgcca                                                                                                                                    <  7:193685/149‑6 (MQ=255)
             cGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGTGTCTGCCGGGTCAg                                                                                                                                   <  8:28079/149‑1 (MQ=255)
              gCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAg                                                                                                                                   >  1:281193/1‑148 (MQ=255)
                  cAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACtt                                                                                                                              <  6:230947/149‑1 (MQ=255)
                   aGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAATCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACtt                                                                                                                              >  2:91737/1‑148 (MQ=255)
                      ccGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAAt                                                                                                                          <  4:63699/149‑1 (MQ=255)
                          gcTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCTTCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGgcc                                                                                                                      <  2:270585/149‑1 (MQ=255)
                              ggCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGgccgc                                                                                                                    <  3:212948/147‑1 (MQ=255)
                              ggCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGgccgc                                                                                                                    <  2:281193/147‑1 (MQ=255)
                                     ttttCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGtt                                                                                                            <  1:286731/148‑1 (MQ=255)
                                     ttttCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGtt                                                                                                            <  2:91460/148‑1 (MQ=255)
                                          tttcGGCTTCTTGGGCTTCTTCGGTGTCTTGATAACCTGCCCACTGGCATCGGTCATAGGCACCCGGTGCTAGGGGATGAAATCAGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCACCAGTTGCACt                                                                                                       >  1:48089/1‑148 (MQ=255)
                                            tcGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgcgcca                                                                                                                                    <  2:12049/117‑6 (MQ=255)
                                            tcGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACATGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgcgcca                                                                                                                                    >  1:12049/1‑112 (MQ=255)
                                              ggCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCca                                                                                                                  >  4:80446/1‑133 (MQ=255)
                                              ggCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCca                                                                                                                  <  3:80446/133‑1 (MQ=255)
                                              ggCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCAt                                                                                                   <  1:10700/148‑1 (MQ=255)
                                                         cttcttCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCacacac                                                                                         <  2:66798/147‑1 (MQ=255)
                                                         cttcttCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCCGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCacacac                                                                                         >  1:66798/1‑147 (MQ=255)
                                                            cttcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCCCTTCATCCGCACACACCAGc                                                                                     >  8:49567/1‑148 (MQ=255)
                                                             ttcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGc                                                                                               >  2:27889/1‑137 (MQ=255)
                                                             ttcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGc                                                                                               <  1:27889/137‑1 (MQ=255)
                                                              tcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGcacac                                                                                           <  8:295642/140‑1 (MQ=255)
                                                              tcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGcacac                                                                                           >  7:295642/1‑140 (MQ=255)
                                                              tcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGca                                                                                              <  2:77262/137‑1 (MQ=255)
                                                              tcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGATGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGca                                                                                              >  1:77262/1‑137 (MQ=255)
                                                                 gTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAAtc                                                                               <  3:258303/149‑1 (MQ=255)
                                                                  tttCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAAtcg                                                                              >  3:132629/1‑149 (MQ=255)
                                                                        gATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAAtcgcctcgc                                                                        >  6:54664/1‑149 (MQ=255)
                                                                             ccTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCttg                                                                   >  1:343259/1‑149 (MQ=255)
                                                                                 cccACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCttgtt                                                                 <  2:74572/147‑1 (MQ=255)
                                                                                        gCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTgcccgcccg                                                        <  5:46497/149‑1 (MQ=255)
                                                                                         cATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTgcccgcccgc                                                       >  2:237024/1‑149 (MQ=255)
                                                                                          aTCGTTCATCGGCACCCGGCGCTCGGGGATGAAATCCGGCTGTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCttgtt                                                                 <  6:107866/138‑1 (MQ=255)
                                                                                          aTCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCATTGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGATgcccgcccgcc                                                      >  2:122427/1‑149 (MQ=255)
                                                                                          aTCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTgcccgcccgcc                                                      <  1:91737/149‑1 (MQ=255)
                                                                                           tCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTgcccgcccgccc                                                     >  5:270590/1‑149 (MQ=255)
                                                                                              gTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCgg                                                   >  8:285073/1‑148 (MQ=255)
                                                                                                                  ggggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGGCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACg                               <  2:343259/148‑1 (MQ=255)
                                                                                                                   gggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACg                               >  6:201115/1‑147 (MQ=255)
                                                                                                                   gggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTa                             <  4:132629/149‑1 (MQ=255)
                                                                                                                   gggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTa                             <  2:79109/149‑1 (MQ=255)
                                                                                                                    ggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGAt                                         >  4:4784/1‑136 (MQ=255)
                                                                                                                    ggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGAt                                         <  3:4784/136‑1 (MQ=255)
                                                                                                                    ggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAg                            <  1:122427/149‑1 (MQ=255)
                                                                                                                              cGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCaa                  <  5:54664/149‑1 (MQ=255)
                                                                                                                               ggCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGc                                       >  4:141784/1‑127 (MQ=255)
                                                                                                                               ggCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGc                                       <  3:141784/127‑1 (MQ=255)
                                                                                                                                  tctTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCg                      <  5:273213/141‑1 (MQ=255)
                                                                                                                                  tctTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCg                      >  6:273213/1‑141 (MQ=255)
                                                                                                                                     tCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACATAGTCTTCGGCAACGATCgg           >  1:173315/1‑149 (MQ=255)
                                                                                                                                       aTGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCgg           >  1:222629/1‑147 (MQ=255)
                                                                                                                                              ggCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCGGCAGGTCGAg  <  1:237024/149‑1 (MQ=255)
                                                                                                                                              ggCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCGGCAGGTCGAg  <  2:222629/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
GCTGTCCATCCAACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCGGCAGGTCGAG  >  NC_002947/5426858‑5427148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑