Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,427,007 T→C 8.0% T366A (ACC→GCC) ‡ rhlE‑I ← ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,427,0070TC 8.0% 171.8 / 4.0 62T366A (ACC→GCC) ‡rhlE‑IATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base T (26/31);  new base C (2/3);  total (28/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCATCCAACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCGGCAGGTCGAG  >  NC_002947/5426862‑5427148
                                                                                                                                                 |                                                                                                                                             
tccatccaACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTg                                                                                                                                            >  1:91460/1‑149 (MQ=255)
  catccaACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGcc                                                                                                                                          >  3:63699/1‑149 (MQ=255)
      caACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGt                                                                                                                                      >  5:210677/1‑149 (MQ=255)
       aaCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGt                                                                                                                                      >  2:286731/1‑148 (MQ=255)
       aaCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgc                                                                                                                                        <  3:328000/146‑2 (MQ=255)
       aaCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgc                                                                                                                                        >  4:328000/1‑145 (MQ=255)
        aCGCGCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCa                                                                                                                                    <  5:156467/149‑1 (MQ=255)
        aCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCa                                                                                                                                    <  8:70171/149‑1 (MQ=255)
        aCGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgcgcca                                                                                                                                    <  7:193685/149‑6 (MQ=255)
         cGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGTGTCTGCCGGGTCAg                                                                                                                                   <  8:28079/149‑1 (MQ=255)
          gCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAg                                                                                                                                   >  1:281193/1‑148 (MQ=255)
              cAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACtt                                                                                                                              <  6:230947/149‑1 (MQ=255)
               aGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAATCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACtt                                                                                                                              >  2:91737/1‑148 (MQ=255)
                  ccGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAAt                                                                                                                          <  4:63699/149‑1 (MQ=255)
                      gcTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCTTCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGgcc                                                                                                                      <  2:270585/149‑1 (MQ=255)
                          ggCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGgccgc                                                                                                                    <  3:212948/147‑1 (MQ=255)
                          ggCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGgccgc                                                                                                                    <  2:281193/147‑1 (MQ=255)
                                 ttttCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGtt                                                                                                            <  1:286731/148‑1 (MQ=255)
                                 ttttCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGtt                                                                                                            <  2:91460/148‑1 (MQ=255)
                                      tttcGGCTTCTTGGGCTTCTTCGGTGTCTTGATAACCTGCCCACTGGCATCGGTCATAGGCACCCGGTGCTAGGGGATGAAATCAGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCACCAGTTGCACt                                                                                                       >  1:48089/1‑148 (MQ=255)
                                        tcGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgcgcca                                                                                                                                    <  2:12049/117‑6 (MQ=255)
                                        tcGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACATGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGACCCTGCCgcgcca                                                                                                                                    >  1:12049/1‑112 (MQ=255)
                                          ggCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCca                                                                                                                  >  4:80446/1‑133 (MQ=255)
                                          ggCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCca                                                                                                                  <  3:80446/133‑1 (MQ=255)
                                          ggCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCAt                                                                                                   <  1:10700/148‑1 (MQ=255)
                                                     cttcttCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCacacac                                                                                         <  2:66798/147‑1 (MQ=255)
                                                     cttcttCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCCGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCacacac                                                                                         >  1:66798/1‑147 (MQ=255)
                                                        cttcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCCCTTCATCCGCACACACCAGc                                                                                     >  8:49567/1‑148 (MQ=255)
                                                         ttcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGc                                                                                               <  1:27889/137‑1 (MQ=255)
                                                         ttcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGc                                                                                               >  2:27889/1‑137 (MQ=255)
                                                          tcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGcacac                                                                                           <  8:295642/140‑1 (MQ=255)
                                                          tcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGcacac                                                                                           >  7:295642/1‑140 (MQ=255)
                                                          tcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGca                                                                                              <  2:77262/137‑1 (MQ=255)
                                                          tcGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGATGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGca                                                                                              >  1:77262/1‑137 (MQ=255)
                                                             gTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAAtc                                                                               <  3:258303/149‑1 (MQ=255)
                                                              tttCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAAtcg                                                                              >  3:132629/1‑149 (MQ=255)
                                                                    gATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAAtcgcctcgc                                                                        >  6:54664/1‑149 (MQ=255)
                                                                         ccTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCttg                                                                   >  1:343259/1‑149 (MQ=255)
                                                                             cccACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCttgtt                                                                 <  2:74572/147‑1 (MQ=255)
                                                                                    gCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTgcccgcccg                                                        <  5:46497/149‑1 (MQ=255)
                                                                                     cATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTgcccgcccgc                                                       >  2:237024/1‑149 (MQ=255)
                                                                                      aTCGTTCATCGGCACCCGGCGCTCGGGGATGAAATCCGGCTGTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCttgtt                                                                 <  6:107866/138‑1 (MQ=255)
                                                                                      aTCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCATTGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGATgcccgcccgcc                                                      >  2:122427/1‑149 (MQ=255)
                                                                                      aTCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTgcccgcccgcc                                                      <  1:91737/149‑1 (MQ=255)
                                                                                       tCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTgcccgcccgccc                                                     >  5:270590/1‑149 (MQ=255)
                                                                                          gTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCgg                                                   >  8:285073/1‑148 (MQ=255)
                                                                                                              ggggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGGCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACg                               <  2:343259/148‑1 (MQ=255)
                                                                                                               gggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACg                               >  6:201115/1‑147 (MQ=255)
                                                                                                               gggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTa                             <  2:79109/149‑1 (MQ=255)
                                                                                                               gggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTa                             <  4:132629/149‑1 (MQ=255)
                                                                                                                ggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGAt                                         >  4:4784/1‑136 (MQ=255)
                                                                                                                ggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGAt                                         <  3:4784/136‑1 (MQ=255)
                                                                                                                ggATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAg                            <  1:122427/149‑1 (MQ=255)
                                                                                                                          cGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCaa                  <  5:54664/149‑1 (MQ=255)
                                                                                                                           ggCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGc                                       <  3:141784/127‑1 (MQ=255)
                                                                                                                           ggCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGc                                       >  4:141784/1‑127 (MQ=255)
                                                                                                                              tctTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCg                      <  5:273213/141‑1 (MQ=255)
                                                                                                                              tctTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCg                      >  6:273213/1‑141 (MQ=255)
                                                                                                                                 tCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACATAGTCTTCGGCAACGATCgg           >  1:173315/1‑149 (MQ=255)
                                                                                                                                   aTGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCgg           >  1:222629/1‑147 (MQ=255)
                                                                                                                                          ggCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCGGCAGGTCGAg  <  1:237024/149‑1 (MQ=255)
                                                                                                                                          ggCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCGGCAGGTCGAg  <  2:222629/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TCCATCCAACGCCCCAGCCCGCGCTTGGCGCTGTTTTCTTTCGGCTTCTTGGGCTTCTTCGGTTTCTTGATAACCTGCCCACTGGCATCGGTCATCGGCACCCGGTGCTCGGGGATGAAATCCGGCTCTTCATGGCGCGGCAGGGTCTGCCGGGTCAGCACTTCAATGGCCGCCAGCAGTTGCACTTCATCCGCACACACCAGCGAAATCGCCTCGCCCTTGTTGCCCGCCCGCCCGGTACGCCCGATGCGGTGCACGTAGTCTTCGGCAACGATCGGCAGGTCGAG  >  NC_002947/5426862‑5427148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑