| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 459,899 | C→T | 7.6% | intergenic (‑45/+34) | pqqB ← / ← pqqA | coenzyme PQQ synthesis protein B/coenzyme PQQ synthesis protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 459,899 | 0 | C | T | 7.6% | 224.8 / 5.5 | 78 | intergenic (‑45/+34) | pqqB/pqqA | coenzyme PQQ synthesis protein B/coenzyme PQQ synthesis protein A |
| Reads supporting (aligned to +/- strand): ref base C (36/36); new base T (2/4); total (38/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTCAGGGTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTC > NC_002947/459756‑460039 | tttCAGGGTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCgg > 5:325212/1‑147 (MQ=255)tttCAGGGTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCgg > 2:262203/1‑147 (MQ=255) aTCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCTCGGCCTGCCGGGGCGtt > 2:57181/1‑148 (MQ=255) tCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGa < 6:235103/149‑1 (MQ=255) aCGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCTCGGCCTGCCGGGGCGttttc < 3:138863/149‑5 (MQ=255) aCGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGaaa < 5:87881/149‑1 (MQ=255) aCGGTAGCCCGTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTTGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCTCGGCCTGCCGGGGCGttttc < 8:239358/149‑5 (MQ=255) tAGCCCTTGAAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCCCGACCCTACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCTCGGCCTGCCGGGGCGtttt < 1:115525/149‑4 (MQ=255) ccTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGc < 7:280146/149‑1 (MQ=255) ccTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGc > 5:60316/1‑149 (MQ=255) ccTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGACGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGc > 1:75531/1‑149 (MQ=255) gCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTg < 5:345680/148‑1 (MQ=255) gTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCt > 6:82322/1‑148 (MQ=255) gACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCa > 7:284317/1‑116 (MQ=255) gACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCa < 8:284317/116‑1 (MQ=255) aCGCATTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCg < 5:51578/149‑1 (MQ=255) aCGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCg < 5:46487/149‑1 (MQ=255) gcagttgcagttCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGCACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGCGGCGTGCACCCCTAAGCCTGGCTTCGCGGt > 4:101164/1‑149 (MQ=255) cagttgcagttCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGt > 8:235000/1‑148 (MQ=255) agttCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAg < 7:235000/149‑1 (MQ=255) gttCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGt < 6:60316/149‑1 (MQ=255) ccACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTAc < 2:183032/148‑1 (MQ=255) aCTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGAGTGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa > 1:240422/1‑138 (MQ=255) aCTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa < 2:240422/137‑1 (MQ=255) aCTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATg > 2:115742/1‑149 (MQ=255) cGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGa > 5:315830/1‑149 (MQ=255) cGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGAACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGt < 1:115742/147‑1 (MQ=255) gggAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGCTCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGAc < 6:262528/149‑1 (MQ=255) ccG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCg > 6:262099/1‑147 (MQ=255) ccG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGaa < 2:41787/149‑1 (MQ=255) cG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGGGGTTAGCGAAGTACAGGGTGACGTCGAAg > 4:251389/1‑149 (MQ=255) cc‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGcc > 8:199974/1‑149 (MQ=255) ccGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACTGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGc < 8:86763/145‑1 (MQ=255) ccGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACGGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGc > 7:86763/1‑145 (MQ=255) ggcggcggAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATAc > 2:116988/1‑149 (MQ=255) ggAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGt > 3:64470/1‑149 (MQ=255) cGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCa > 5:7603/1‑146 (MQ=255) cGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCa < 6:7603/146‑1 (MQ=255) gagaACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATg > 5:145542/1‑118 (MQ=255) gagaACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATg < 6:145542/118‑1 (MQ=255) gagaACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAgtgt < 1:116988/149‑1 (MQ=255) cTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAg < 5:82322/149‑1 (MQ=255) cTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAg > 5:85230/1‑149 (MQ=255) cTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAg < 5:262099/149‑1 (MQ=255) gA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAgtgt < 6:77128/140‑1 (MQ=255) gA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAgtgt > 5:77128/1‑140 (MQ=255) gA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGtt < 3:251389/149‑1 (MQ=255) a‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGttt < 6:325212/149‑1 (MQ=255) tGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTg > 6:11711/1‑149 (MQ=255) tGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTg > 6:16599/1‑149 (MQ=255) aCATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGTAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGAt < 2:82875/121‑1 (MQ=255) aCATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGTAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGAt > 1:82875/1‑121 (MQ=255) aTGGCCACTCTCTATGCAGAAACCGGATCGAAAAATACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAgt > 1:225294/1‑132 (MQ=255) aTGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAgt < 2:225294/131‑1 (MQ=255) tGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCca > 5:250347/1‑148 (MQ=255) ggCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGaa < 6:50326/111‑1 (MQ=255) ggCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGaa > 5:50326/1‑111 (MQ=255) tATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACt > 3:315182/1‑149 (MQ=255) tGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGTCTTCGAAGCCGATACGCAGGTCTGTGTATGCAGGTTTGGTCCACATGGAATTACTcc < 6:57804/149‑1 (MQ=255) tGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTcc > 7:106472/1‑149 (MQ=255) tGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTcc < 7:199974/149‑1 (MQ=255) aGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTATCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTc < 3:101164/149‑1 (MQ=255) aGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTc < 4:64470/149‑1 (MQ=255) aGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTc > 6:72336/1‑149 (MQ=255) gAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGATGCCGATACGCAGGTCAGTGTATGCAGGTTTTGTCCACATGGAATTACTCCTTcc < 1:262203/149‑1 (MQ=255) gAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTcc < 6:250347/149‑1 (MQ=255) ggATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAAt > 1:153776/1‑147 (MQ=255) gATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGt > 4:175273/1‑72 (MQ=255) gATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGt < 3:175273/72‑1 (MQ=255) aaaaa‑aCGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgggttg > 2:6727/1‑149 (MQ=255) aaaaa‑aCGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgggttg > 5:9873/1‑149 (MQ=255) aaaaa‑aCGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgggttg > 5:254988/1‑149 (MQ=255) aaa‑acgaaaacgCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgggttggg < 6:85230/149‑1 (MQ=255) ttcacgCCTCGGCCTGCCGGGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCt < 4:26204/123‑1 (MQ=255) ttcacgCCTCGGCCTGCCGGGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCt > 3:26204/4‑126 (MQ=255) aaaacgCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTc < 4:315182/149‑1 (MQ=255) gCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgg > 7:158273/1‑132 (MQ=255) gCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgg < 8:158273/132‑1 (MQ=255) | TTTCAGGGTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAA‑‑CCCG‑‑CC‑ACCGGCGGCGGAGCCGAGAACCTGGA‑TGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAA‑ACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTC > NC_002947/459756‑460039 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |