Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,452,346 C→T 7.4% R293C (CGC→TGC) ‡ lnt → apolipoprotein N‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,452,3460CT 7.4% 153.2 / 2.3 54R293C (CGC→TGC) ‡lntapolipoprotein N‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (24/25);  new base T (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01

GCACATCGACGCGCAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAGTATGTGCCGCTG  >  NC_002947/5452198‑5452492
                                                                                                                                                    |                                                                                                                                                  
gcACATCGACGCGCAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAgc                                                                                                                                                    <  8:43505/149‑1 (MQ=255)
    aTCGACGCGCAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCa                                                                                                                                                >  5:99292/1‑149 (MQ=255)
      cGACGCGCAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACt                                                                                                                                              >  2:267290/1‑149 (MQ=255)
           cgcAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGgcg                                                                                                                                         <  6:246183/149‑1 (MQ=255)
            gcAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGTGGCGCTCGgcgg                                                                                                                                        <  2:248982/149‑2 (MQ=255)
                  ggCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCa                                                                                                                                  >  3:43902/1‑149 (MQ=255)
                   gcgcTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCAc                                                                                                                                 >  2:86921/1‑149 (MQ=255)
                     gcTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACgg                                                                                                                               <  4:41647/149‑1 (MQ=255)
                     gcTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACgg                                                                                                                               <  6:267975/149‑1 (MQ=255)
                         taCCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGt                                                                                                                           <  3:2570/149‑1 (MQ=255)
                           ccGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGc                                                                                                                         <  1:267290/149‑1 (MQ=255)
                            cgcgACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACgg                                                                                                                               <  7:53597/142‑1 (MQ=255)
                            cgcgACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACgg                                                                                                                               >  8:53597/1‑142 (MQ=255)
                             gcgcCCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATAACGGGCGTGACg                                                                                                                       >  5:138/1‑149 (MQ=255)
                             gcgACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCg                                                                                                                       >  5:103116/1‑149 (MQ=255)
                              cgACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCgg                                                                                                                      >  6:38547/1‑149 (MQ=255)
                               gACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGt                                                                                                                     <  4:108226/149‑1 (MQ=255)
                                 ccTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGc                                                                                                                   <  5:158363/149‑1 (MQ=255)
                                  cTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGTCGCTGATCACGGGCGTGCCGGTGCg                                                                                                                  >  7:140461/1‑149 (MQ=255)
                                     aGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGt                                                                                                                 <  6:99292/147‑1 (MQ=255)
                                     aGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTg                                                                                                                <  6:103116/148‑1 (MQ=255)
                                          cAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTgaagaa                                                                                                           <  7:191376/148‑1 (MQ=255)
                                          cAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTgaagaa                                                                                                           <  1:86921/148‑1 (MQ=255)
                                               ccAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGcac                                                                                                     >  1:233236/1‑149 (MQ=255)
                                                               gctgGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTgaa                                                                                                              >  7:48078/1‑124 (MQ=255)
                                                               gctgGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTgaa                                                                                                              <  8:48078/124‑1 (MQ=255)
                                                                ctgGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGctacta                                                                                    <  5:38547/149‑1 (MQ=255)
                                                                ctgGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGctac                                                                                      <  4:43902/147‑1 (MQ=255)
                                                                          cGGAAACCGCCGTGCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATc                                                                          <  5:273802/149‑1 (MQ=255)
                                                                                acgccgTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCAACGt                                                                     >  8:14125/2‑148 (MQ=255)
                                                                                    cgTTCCGGTGCTCACGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTg                                                                <  7:14125/149‑1 (MQ=255)
                                                                                        ccGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCg                                                             >  5:250864/1‑148 (MQ=255)
                                                                                          ggTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGgcgc                                                                                                                                        >  2:314502/1‑71 (MQ=255)
                                                                                          ggTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGgcgc                                                                                                                                        <  1:314502/71‑1 (MQ=255)
                                                                                           gTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAgg                                                         <  3:226687/149‑1 (MQ=255)
                                                                                               tCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGAc                                                     >  5:170269/1‑149 (MQ=255)
                                                                                                cAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACg                                                    >  1:124056/1‑149 (MQ=255)
                                                                                                      caaGGCCCGGGGCTACATCGAAGTGATGTGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCacc                                               >  7:57641/3‑148 (MQ=255)
                                                                                                       cAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCaccta                                             >  4:57331/1‑149 (MQ=255)
                                                                                                        aGGCCCGGGGCTACATCGACGTTATGTGCCGATTAGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTTAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCacctac                                            <  8:57641/149‑1 (MQ=255)
                                                                                                          gCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCacctacct                                          >  1:153863/1‑149 (MQ=255)
                                                                                                            ccAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACGAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTc                                         >  5:75755/1‑148 (MQ=255)
                                                                                                            ccAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTc                                         >  5:255898/1‑148 (MQ=255)
                                                                                                            ccAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTc                                         >  8:53088/1‑148 (MQ=255)
                                                                                                               gggCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGc                                     <  3:57331/149‑1 (MQ=255)
                                                                                                                        cGACGTCATGGGCCGCTTGGCGGCCGTGAGCGACTTGGCGCTGTTTACGGGCGTGCCGGTGCGTGATGAAGTGCACCACAATTGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTg                             <  1:276663/148‑1 (MQ=255)
                                                                                                                                tGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCAttt                    <  8:140461/149‑1 (MQ=255)
                                                                                                                                  ggCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACGAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTg                   <  6:75755/148‑1 (MQ=255)
                                                                                                                                    ccGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGc                 >  7:149041/1‑148 (MQ=255)
                                                                                                                                     cGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGa               <  1:157364/149‑1 (MQ=255)
                                                                                                                                       cTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAGt             <  6:250864/149‑1 (MQ=255)
                                                                                                                                         tcagcgccgAGTGGCGCTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAg              >  8:70599/5‑146 (MQ=255)
                                                                                                                                         tcagcgccgAGTGGCGCTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAg              <  7:70599/142‑1 (MQ=255)
                                                                                                                                            gcgccgAGTGGCGCTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAGTAtgtg        >  2:47896/2‑149 (MQ=255)
                                                                                                                                            ccgccgAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAGTAtgtg        >  6:196403/1‑149 (MQ=255)
                                                                                                                                                   gcgcCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAGTATGTGCCGCTg  >  5:29928/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCACATCGACGCGCAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAGTATGTGCCGCTG  >  NC_002947/5452198‑5452492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑