Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,452,348 C→G 7.4% R293R (CGC→CGG) ‡ lnt → apolipoprotein N‑acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,452,3480CG 7.4% 166.3 / 3.4 54R293R (CGC→CGG) ‡lntapolipoprotein N‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (24/26);  new base G (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

ATCGACGCGCAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAGTATGTGCCGCTG  >  NC_002947/5452202‑5452492
                                                                                                                                                  |                                                                                                                                                
aTCGACGCGCAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCa                                                                                                                                                >  5:99292/1‑149 (MQ=255)
  cGACGCGCAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACt                                                                                                                                              >  2:267290/1‑149 (MQ=255)
       cgcAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGgcg                                                                                                                                         <  6:246183/149‑1 (MQ=255)
        gcAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGTGGCGCTCGgcgg                                                                                                                                        <  2:248982/149‑2 (MQ=255)
              ggCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCa                                                                                                                                  >  3:43902/1‑149 (MQ=255)
               gcgcTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCAc                                                                                                                                 >  2:86921/1‑149 (MQ=255)
                 gcTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACgg                                                                                                                               <  4:41647/149‑1 (MQ=255)
                 gcTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACgg                                                                                                                               <  6:267975/149‑1 (MQ=255)
                     taCCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGt                                                                                                                           <  3:2570/149‑1 (MQ=255)
                       ccGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGc                                                                                                                         <  1:267290/149‑1 (MQ=255)
                        cgcgACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACgg                                                                                                                               <  7:53597/142‑1 (MQ=255)
                        cgcgACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACgg                                                                                                                               >  8:53597/1‑142 (MQ=255)
                         gcgcCCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATAACGGGCGTGACg                                                                                                                       >  5:138/1‑149 (MQ=255)
                         gcgACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCg                                                                                                                       >  5:103116/1‑149 (MQ=255)
                          cgACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCgg                                                                                                                      >  6:38547/1‑149 (MQ=255)
                           gACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGt                                                                                                                     <  4:108226/149‑1 (MQ=255)
                             ccTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGc                                                                                                                   <  5:158363/149‑1 (MQ=255)
                              cTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGTCGCTGATCACGGGCGTGCCGGTGCg                                                                                                                  >  7:140461/1‑149 (MQ=255)
                                 aGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGt                                                                                                                 <  6:99292/147‑1 (MQ=255)
                                 aGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTg                                                                                                                <  6:103116/148‑1 (MQ=255)
                                      cAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTgaagaa                                                                                                           <  7:191376/148‑1 (MQ=255)
                                      cAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTgaagaa                                                                                                           <  1:86921/148‑1 (MQ=255)
                                           ccAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGcac                                                                                                     >  1:233236/1‑149 (MQ=255)
                                                           gctgGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTgaa                                                                                                              <  8:48078/124‑1 (MQ=255)
                                                           gctgGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTgaa                                                                                                              >  7:48078/1‑124 (MQ=255)
                                                            ctgGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGctacta                                                                                    <  5:38547/149‑1 (MQ=255)
                                                            ctgGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGctac                                                                                      <  4:43902/147‑1 (MQ=255)
                                                                      cGGAAACCGCCGTGCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATc                                                                          <  5:273802/149‑1 (MQ=255)
                                                                            acgccgTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCAACGt                                                                     >  8:14125/2‑148 (MQ=255)
                                                                                cgTTCCGGTGCTCACGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTg                                                                <  7:14125/149‑1 (MQ=255)
                                                                                    ccGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCg                                                             >  5:250864/1‑148 (MQ=255)
                                                                                      ggTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGgcgc                                                                                                                                        >  2:314502/1‑71 (MQ=255)
                                                                                      ggTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGgcgc                                                                                                                                        <  1:314502/71‑1 (MQ=255)
                                                                                       gTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAgg                                                         <  3:226687/149‑1 (MQ=255)
                                                                                           tCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGAc                                                     >  5:170269/1‑149 (MQ=255)
                                                                                            cAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACg                                                    >  1:124056/1‑149 (MQ=255)
                                                                                                  caaGGCCCGGGGCTACATCGAAGTGATGTGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCacc                                               >  7:57641/3‑148 (MQ=255)
                                                                                                   cAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCaccta                                             >  4:57331/1‑149 (MQ=255)
                                                                                                    aGGCCCGGGGCTACATCGACGTTATGTGCCGATTAGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTTAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCacctac                                            <  8:57641/149‑1 (MQ=255)
                                                                                                      gCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCacctacct                                          >  1:153863/1‑149 (MQ=255)
                                                                                                        ccAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACGAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTc                                         >  5:75755/1‑148 (MQ=255)
                                                                                                        ccAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTc                                         >  5:255898/1‑148 (MQ=255)
                                                                                                        ccAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTc                                         >  8:53088/1‑148 (MQ=255)
                                                                                                           gggCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGc                                     <  3:57331/149‑1 (MQ=255)
                                                                                                                    cGACGTCATGGGCCGCTTGGCGGCCGTGAGCGACTTGGCGCTGTTTACGGGCGTGCCGGTGCGTGATGAAGTGCACCACAATTGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTg                             <  1:276663/148‑1 (MQ=255)
                                                                                                                            tGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCAttt                    <  8:140461/149‑1 (MQ=255)
                                                                                                                              ggCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACGAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTg                   <  6:75755/148‑1 (MQ=255)
                                                                                                                                ccGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGc                 >  7:149041/1‑148 (MQ=255)
                                                                                                                                 cGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGa               <  1:157364/149‑1 (MQ=255)
                                                                                                                                   cTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAGt             <  6:250864/149‑1 (MQ=255)
                                                                                                                                     tcagcgccgAGTGGCGCTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAg              <  7:70599/142‑1 (MQ=255)
                                                                                                                                     tcagcgccgAGTGGCGCTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAg              >  8:70599/5‑146 (MQ=255)
                                                                                                                                        gcgccgAGTGGCGCTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAGTAtgtg        >  2:47896/2‑149 (MQ=255)
                                                                                                                                        ccgccgAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAGTAtgtg        >  6:196403/1‑149 (MQ=255)
                                                                                                                                               gcgcCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAGTATGTGCCGCTg  >  5:29928/1‑148 (MQ=255)
                                                                                                                                                  ccACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACgg                                                   >  2:282182/1‑96 (MQ=255)
                                                                                                                                                  ccACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACgg                                                   <  1:282182/96‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
ATCGACGCGCAACTGGCGCTATACCGCGACCTGAGCTTCAGCTCCAGGCCGGTGGACCTGCTGGTGTGGCCGGAAACCGCCGTTCCGGTGCTCAAGGACCAGGCCCAGGGCTACATCGACGTGATGGGCCGCTTCGCCGCCGAGCGCCACTCGGCGCTGATCACGGGCGTGCCGGTGCGTGAAGAAGTGCACCACCAGCGCCGCTACTACAACGGCATCACCGTCACTGGCGAAGGCGACGGCACCTACCTCAAGCAGAAGCTGGTGCCATTTGGCGAGTATGTGCCGCTG  >  NC_002947/5452202‑5452492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑