Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,461,652 G→A 5.7% A198T (GCG→ACG)  PP_4798 → membrane‑bound lytic murein transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,461,6520GA 5.7% 214.4 / 2.1 70A198T (GCG→ACG) PP_4798membrane‑bound lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base G (28/38);  new base A (2/2);  total (30/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.79e-01

TGGCGTGGACCGCCAGGTGCTGGTCGCGGTGTGGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGACTTCGACGGCGATGGCCGCCGCGACATCTGG  >  NC_002947/5461504‑5461798
                                                                                                                                                    |                                                                                                                                                  
tGGCGTGGACCGCCAGGTGCTGGTCGCGGTGTGGGGCATGGAAAGCAACTTCGTCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCcg                                                                                                                                                    <  8:264442/149‑1 (MQ=255)
     tGGACCGCCAGGTGCTGGTCGCGGTGTGGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCTCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTg                                                                                                                                               <  6:337865/149‑1 (MQ=255)
     tGGACCGCCAGGTGCTGGTCGCGGTGTGGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTg                                                                                                                                               <  6:120803/149‑1 (MQ=255)
     tGGACCGCCAGGTGCTGGTCGCGGTGTGGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTg                                                                                                                                               <  3:267790/149‑1 (MQ=255)
        aCCGCCAGGTGCTGGTCGCGGTGTGGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTg                                                                                                                                               >  7:266330/1‑146 (MQ=255)
          cGCCAGGTGCTGGTCGCGGTGTGGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTg                                                                                                                                               >  5:347542/1‑144 (MQ=255)
          cGCCAGGTGCTGGTCGCGGTGTGGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTg                                                                                                                                               >  6:136901/1‑144 (MQ=255)
           gCCAGGTGCTGGGCGCGGGGTGGGGCATGGAAATCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGCGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGatc                                                                                                                                         <  8:184638/149‑1 (MQ=255)
              aGGTGCTGGTCGCGGTGTGGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGatcatc                                                                                                                                      <  3:171578/149‑1 (MQ=255)
                       tCGCGGTGTGGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGt                                                                                                                             >  2:330938/1‑149 (MQ=255)
                          cgGTGTGGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGAc                                                                                                                          <  3:25616/149‑1 (MQ=255)
                               tgGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATcc                                                                                                                      <  7:39709/148‑1 (MQ=255)
                               tgGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATc                                                                                                                       >  5:213696/1‑147 (MQ=255)
                                 gggCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAg                                                                                                                    <  7:167079/148‑1 (MQ=255)
                                 gggCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAg                                                                                                                    <  7:57808/148‑1 (MQ=255)
                                  ggCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGcc                                                                                                                  <  1:204927/149‑1 (MQ=255)
                                   gCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGatcat                                                                                                                                       <  3:279699/127‑1 (MQ=255)
                                   gCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGatcat                                                                                                                                       >  4:279699/1‑127 (MQ=255)
                                      tGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGaa                                                                                                              <  1:335672/149‑1 (MQ=255)
                                       ggAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAg                                                                                                             >  6:293724/1‑149 (MQ=255)
                                         aaaGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAAGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGc                                                                                                            >  1:1964/1‑148 (MQ=255)
                                          aaGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCACGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCa                                                                                                          >  3:302948/1‑149 (MQ=255)
                                           aGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCAt                                                                                                         >  6:30896/1‑149 (MQ=255)
                                             cAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGc                                                                                                       >  3:67795/1‑149 (MQ=255)
                                               aCTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGt                                                                                                     <  8:50356/149‑1 (MQ=255)
                                                  tCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGc                                                                                                  >  5:151195/1‑149 (MQ=255)
                                                   cGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTgg                                                                                                   <  2:270115/147‑1 (MQ=255)
                                                     gCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCAt                                                                                                         <  4:58921/139‑1 (MQ=255)
                                                     gCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCAt                                                                                                         >  3:58921/1‑139 (MQ=255)
                                                                ggCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGgcgc                                                                                    >  7:268932/1‑149 (MQ=255)
                                                                    gcaAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATg                                                                                >  2:76570/1‑149 (MQ=255)
                                                                    gcaAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATg                                                                                >  2:71661/1‑149 (MQ=255)
                                                                     caAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATgg                                                                               <  6:213696/149‑1 (MQ=255)
                                                                     caAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGATGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATgg                                                                               <  7:220416/149‑1 (MQ=255)
                                                                           ggTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGa                                                                         <  5:30896/149‑1 (MQ=255)
                                                                             tGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGAcc                                                                       <  4:253105/149‑1 (MQ=255)
                                                                              gATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGAcc                                                                       >  5:151138/1‑148 (MQ=255)
                                                                                tCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAg                                                                    <  5:136901/149‑1 (MQ=255)
                                                                                  cgctcgctGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGt                                                                   <  4:67795/148‑1 (MQ=255)
                                                                                   gctcgctGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTg                                                                                                    >  7:239936/1‑114 (MQ=255)
                                                                                   gctcgctGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTg                                                                                                    <  8:239936/114‑1 (MQ=255)
                                                                                   gctcgctGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCACGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTc                                                                 <  4:302948/149‑1 (MQ=255)
                                                                                       gctGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATcc                                                             <  6:151195/149‑1 (MQ=255)
                                                                                        ctGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGTGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATccc                                                            <  6:347542/149‑1 (MQ=255)
                                                                                               cTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGaccacc                                                     <  1:76570/149‑1 (MQ=255)
                                                                                               cTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGaccacc                                                     <  1:71661/149‑1 (MQ=255)
                                                                                                  tGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGt                                                                                                                             >  4:251955/1‑74 (MQ=255)
                                                                                                  tGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGt                                                                                                                             <  3:251955/74‑1 (MQ=255)
                                                                                                    gCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCACGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTacaa                                                >  8:242556/1‑149 (MQ=255)
                                                                                                       tATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAacac                                             >  8:20662/1‑149 (MQ=255)
                                                                                                         tGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGac                                                         <  8:127501/135‑1 (MQ=255)
                                                                                                         tGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGac                                                         >  7:127501/1‑135 (MQ=255)
                                                                                                            gggCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATg                                        >  6:300836/1‑149 (MQ=255)
                                                                                                               ccgccgccCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAg                                                                                                             <  8:80258/77‑1 (MQ=255)
                                                                                                               ccgccgccCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAg                                                                                                             >  7:80258/1‑77 (MQ=255)
                                                                                                                 gccgccCACAGTTCGCCCAGGATCAGCTGATTGCCACGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTg                                   <  7:242556/149‑1 (MQ=255)
                                                                                                                   cgccCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGa                                 <  7:20662/149‑1 (MQ=255)
                                                                                                                     cccACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGCCt                               >  7:59225/1‑149 (MQ=255)
                                                                                                                           gTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGACTTCGACg                         >  7:263656/1‑149 (MQ=255)
                                                                                                                               gCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGACTTCGACGGCg                      <  5:293724/148‑1 (MQ=255)
                                                                                                                                 ccAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATccc                                                            <  7:86395/108‑1 (MQ=255)
                                                                                                                                 ccAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATccc                                                            >  8:86395/1‑108 (MQ=255)
                                                                                                                                   aGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATTTTTAATACTCATTTCATCACGACCAACTACAACACCCATGCCGTGGACTTCGACGGCGATGgc                 <  2:1964/149‑1 (MQ=255)
                                                                                                                                    ggATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCTAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGACTTCGACGGCGATGgcc                <  7:306993/149‑1 (MQ=255)
                                                                                                                                    ggATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGACTTCGACGGCGATGgcc                <  6:151138/149‑1 (MQ=255)
                                                                                                                                         aGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGACTTCGACGGCGATGGCcgccgc           <  8:263656/149‑1 (MQ=255)
                                                                                                                                          gCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGACTTCGACGGCGATGGCcgccgc           >  1:113141/1‑148 (MQ=255)
                                                                                                                                            tGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGCGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGACTTCGACGGCGATGGCCGCCGCGa         >  1:109729/1‑148 (MQ=255)
                                                                                                                                                  ccGCGCTGCAGATCATCCAGCATGTTGCCATCCCGCCTGAAGCCCTGCGTGGCTCGTGGGCCGGCGCCATGGGCAAGACCCAGTTCATCCCGACCACCTACACCACCCATGCCGTGGACTTCGACTGCGATGGCCGCCGCGACATCTg   <  8:266330/148‑1 (MQ=255)
                                                                                                                                                  ccGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGACTTCGACGGCGATGGCCGCCGCGACATCTgg  <  8:268932/149‑1 (MQ=255)
                                                                                                                                                  ccGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGACTTCGACGGCGATGGCCGCCGCGACATCTg   >  6:324965/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGGCGTGGACCGCCAGGTGCTGGTCGCGGTGTGGGGCATGGAAAGCAACTTCGGCCAGTTCCAGGGCAGCAAGTCGGTGATCCGCTCGCTGGCCACTTTGGCCTATGAGGGCCGCCGCCCACAGTTCGCCCAGGATCAGCTGATTGCCGCGCTGCAGATCATCCAGCATGGTGACATCCAGCCTGAAGCCATGCGTGGCTCGTGGGCCGGCGCCATGGGCCAGACCCAGTTCATCCCGACCACCTACAACACCCATGCCGTGGACTTCGACGGCGATGGCCGCCGCGACATCTGG  >  NC_002947/5461504‑5461798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑