Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,478,782 G→C 7.9% I33M (ATC→ATG)  PP_4816 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,478,7820GC 7.9% 192.8 / 6.7 63I33M (ATC→ATG) PP_4816hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (33/25);  new base C (3/2);  total (36/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-01

AATGTCCTTGGCATATACCATCACCCCGACTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGAT  >  NC_002947/5478635‑5478929
                                                                                                                                                   |                                                                                                                                                    
aaTGTCCTTGGCATATACCATCACCCCGACTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCgatga                                                                                                                                                     >  5:39192/1‑149 (MQ=255)
       ttGGCATATACCATCACCCCGACTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGccc                                                                                                                                              >  4:132355/1‑149 (MQ=255)
           cATATACCATCACCCCGACTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAACTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCggg                                                                                                                                           >  2:250101/1‑148 (MQ=255)
            atatACCATCACCCCGACTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGTTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGt                                                                                                                                          >  3:69571/1‑148 (MQ=255)
             tataCCATCACCCCGACTGCCGGCGGCACCAGCAGCAGCTGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATCATCGGcctgttgtt                                                                                                                                        <  7:244945/149‑8 (MQ=255)
             tataCCATCACCCCGACTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTa                                                                                                                                         >  3:22305/1‑148 (MQ=255)
                aCCATCACCCCGACTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTaaaaa                                                                                                                                     >  1:61252/1‑149 (MQ=255)
                   aTCACCCCGACTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTaaaaa                                                                                                                                     >  1:43605/1‑146 (MQ=255)
                   aTCACCCCGACTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTaaaaa                                                                                                                                     <  2:43605/146‑1 (MQ=255)
                   aTCACCCCGACTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACgg                                                                                                                                  >  3:181814/1‑149 (MQ=255)
                   aTCACCCCGACTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACgg                                                                                                                                  >  5:264703/1‑149 (MQ=255)
                             cTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGTTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAgg                                                                                                                        <  4:69571/149‑1 (MQ=255)
                              tGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCTGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGt                                                                                                                       <  5:271831/149‑1 (MQ=255)
                                 cggcggCACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGtgat                                                                                                                    <  6:39192/149‑1 (MQ=255)
                                    cggcACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGtgattga                                                                                                                 <  1:299424/149‑1 (MQ=255)
                                         ccAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGcc                                                                                                            <  3:140841/149‑1 (MQ=255)
                                                cagcGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCca                                                                                                     <  4:22305/149‑1 (MQ=255)
                                                      gagaTAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCggg                                                                                                                                           <  3:145794/105‑1 (MQ=255)
                                                      gagaTAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCggg                                                                                                                                           >  4:145794/1‑105 (MQ=255)
                                                       agaTAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGGAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTg                                                                                              >  6:78116/1‑149 (MQ=255)
                                                                agcaAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTAc                                                                                     >  4:123958/1‑149 (MQ=255)
                                                                agcaAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTAc                                                                                     <  4:181814/149‑1 (MQ=255)
                                                                  caAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTAcca                                                                                   <  3:132355/149‑1 (MQ=255)
                                                                         ctTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGt                                                                            >  4:27883/1‑149 (MQ=255)
                                                                            gCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCACGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTc                                                                           <  2:228745/147‑1 (MQ=255)
                                                                              ggCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCagcagc                                                                                                >  3:333471/1‑124 (MQ=255)
                                                                              ggCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCagcagc                                                                                                <  4:333471/124‑1 (MQ=255)
                                                                              ggCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAAc                                                                       <  2:41571/149‑1 (MQ=255)
                                                                                ccTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCa                                                                     >  7:227273/1‑149 (MQ=255)
                                                                                ccTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCa                                                                     >  7:175622/1‑149 (MQ=255)
                                                                                  tCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTAccagccag                                                                              >  8:194291/1‑138 (MQ=255)
                                                                                  tCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTAccagccag                                                                              <  7:194291/138‑1 (MQ=255)
                                                                                      tCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCaa                                                                >  4:128968/1‑148 (MQ=255)
                                                                                        aGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAg                                                             >  8:23585/1‑149 (MQ=255)
                                                                                             cTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCAt                                                          >  3:80406/1‑147 (MQ=255)
                                                                                                gCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑c                                                     <  3:123958/148‑1 (MQ=255)
                                                                                                      cTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTTAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTccc                                              >  6:250679/1‑149 (MQ=255)
                                                                                                         aCCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCg                                           >  4:128376/1‑149 (MQ=255)
                                                                                                          ccGCGGGCCATGAGGCAGAGCAGCAGCATCAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAAGCACGCAACAGCATAAG‑CATCTCCCTCgg                                          <  4:320463/149‑1 (MQ=255)
                                                                                                          ccGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCgg                                          <  3:27883/149‑1 (MQ=255)
                                                                                                             cgGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCa                                       >  7:234787/1‑149 (MQ=255)
                                                                                                               ggCCATGAGGGAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATg                                     <  5:78116/149‑1 (MQ=255)
                                                                                                                 ccATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGgc                                   <  8:175622/149‑1 (MQ=255)
                                                                                                                      aGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCa                              >  4:48433/1‑149 (MQ=255)
                                                                                                                        gAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGc                                                            <  6:122876/118‑1 (MQ=255)
                                                                                                                          agagCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATtat                          <  8:227273/149‑1 (MQ=255)
                                                                                                                           gatcagcagcaACAACAGGCCGATGATCGGCCAGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCCGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCAGtat                          >  3:229237/4‑148 (MQ=255)
                                                                                                                               agcagcaACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACTAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACAACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCa                     >  2:59194/1‑149 (MQ=255)
                                                                                                                               agcagcaACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTgg                                                                                             >  4:153512/1‑78 (MQ=255)
                                                                                                                               agcagcaACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTgg                                                                                             <  3:153512/78‑1 (MQ=255)
                                                                                                                                gcagcaACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCAc                    >  2:238872/1‑149 (MQ=255)
                                                                                                                                 cagcaACAACAGGCCGATGATCGGCCAGTGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCAc                    >  7:235875/1‑148 (MQ=255)
                                                                                                                                   gcaACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCt                 >  2:76328/1‑149 (MQ=255)
                                                                                                                                     aacaacAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCg               >  6:232576/1‑149 (MQ=255)
                                                                                                                                     aacaacAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCg               >  5:131185/1‑149 (MQ=255)
                                                                                                                                       acccgggCCGATCATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATg                                     <  7:207694/120‑1 (MQ=255)
                                                                                                                                       acccgggCCGATCATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATg                                     >  8:207694/6‑125 (MQ=255)
                                                                                                                                        aacaGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCt            >  3:335162/1‑149 (MQ=255)
                                                                                                                                        aacaGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCt            >  1:5284/1‑149 (MQ=255)
                                                                                                                                         ccgggCCGATCATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTg           >  6:272553/4‑149 (MQ=255)
                                                                                                                                         ccgggCCGATCATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTg           >  5:110778/4‑149 (MQ=255)
                                                                                                                                         acaGTCCGCTGATCGGCCCGGGTAAAAACGGCACGAACCGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACCGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTg           <  4:229237/149‑1 (MQ=255)
                                                                                                                                              ccGATGAGCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAAGCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGAc      <  6:264703/149‑1 (MQ=255)
                                                                                                                                               cGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAGCCATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGAc      <  1:76328/149‑1 (MQ=255)
                                                                                                                                                gatgatCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCg    <  4:80406/149‑1 (MQ=255)
                                                                                                                                                  tgatCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGat  <  3:128376/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AATGTCCTTGGCATATACCATCACCCCGACTGCCGGCGGCACCAGCAGCAGCGGGAGATAGCGCAGCAAGCTGCTTGCGGCCTCGTTCAGCGGCTTGCCGACCTCACCGCGGGCCATGAGGAAGAGCAGCAGCAACAACAGGCCGATGATCGGCCCGGGTAAAAACGGCACGAACAGGTGATTGATCGCCGTCCCCAGCAGCTGGAACAGTACCAGCCAGGTCAAACCACGCAACAGCATAAG‑CATCTCCCTCGGGCATGGCCGCCATTATAAGCACGCTCGGCTGTAGACCGAT  >  NC_002947/5478635‑5478929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑