| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,485,171 | C→T | 10.1% | intergenic (+77/‑70) | purH → / → purD | bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,485,171 | 0 | C | T | 10.1% | 124.8 / 4.8 | 50 | intergenic (+77/‑70) | purH/purD | bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase/phosphoribosylamine‑‑glycine ligase |
| Reads supporting (aligned to +/- strand): ref base C (23/21); new base T (2/3); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.46e-01 | |||||||||||
ATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCACGCGTCGAGA > NC_002947/5485029‑5485319 | aTCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCATGGCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGCATACGGTGGGGGATTGACCGCCGCCTTcg > 1:80926/1‑149 (MQ=255) tCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGTGGCTTGCGCCGAACACTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTcgc < 7:286367/149‑1 (MQ=14) tCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCACCGCATTcgcg < 1:315726/149‑1 (MQ=255) gctgctGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTCGCgg < 4:62226/148‑1 (MQ=37) tgcCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTCGCGGGCAAGc > 8:200811/1‑149 (MQ=25) ccGACGAGGCCGGCATCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATGCGGCGGTGAATCCACCATCGTATTCGCGGGCAAGc < 7:200811/147‑1 (MQ=25) ccGACGAGGCCGGCATCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGcc < 4:85979/148‑1 (MQ=255) cgacgaGGCCGGCATCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCg < 2:47541/149‑1 (MQ=255) cgacgaGGCCGGCATCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCCCGAATAAGAATGTGGATTCACCCCCGCAATAGCCGGCAAGCCCg > 2:269051/1‑149 (MQ=255) gCCGGCATCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTTCCCGCGAATACGATGGTGGATTAACCGCCGCAATAGCGGGCAAGCCCCCTCCcac > 6:155116/1‑149 (MQ=255) ccGGCATCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTTCCCGCGAATACGATTGGGGATTCACCGCCGCATTAGCGGGCCAGCCCGCGCCcacc > 1:276693/1‑148 (MQ=255) aTCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCGTGGCCCCCAATACGAAGGTGGGATAACAGCCGCCATAACGGGCAAGCCCCCTCCCCCAat‑gg > 4:211974/1‑147 (MQ=255) tCGCTATGGTCTTCACTGGAAT‑GCGCCACTTCCGCCACAAATTACGCGCATCCCGAGCCAAGTCGAGATC‑CTGTGGTCGCGGGCTTGCCCGCTAATACGATGGTGGATTCCCCGCCGCATTCGCGGGAAAGCCCGCTCCCACAGT‑GTTc < 8:191746/149‑1 (MQ=255) cgATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg < 2:328217/123‑1 (MQ=255) cgATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCgg > 1:328217/1‑123 (MQ=255) aCTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACg < 5:337879/148‑1 (MQ=255) aCTTCCGCCACTAATTCCGCGGATCCCGAGGCATGTCTAGATC‑CTGTGGGAGCGGGCTTGCCCGCTAATACTATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATc < 4:261169/149‑1 (MQ=38) ccGCCACTAATTACGCGGATCCCGAGGCACGTCGAGATC‑CTGGGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAg < 5:155116/148‑1 (MQ=255) cgcgGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCTAATACGATGGGGGATTCACCGCCGAATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGaaa < 1:269051/149‑1 (MQ=255) tCCCGAGCCAAGTCGATATC‑CTGGGGGAGCGGGCTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTg < 2:80926/149‑1 (MQ=255) aGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGatcat < 5:68943/149‑1 (MQ=255) ccAAGTCGATATC‑CTGTGGGCGCGGGCTTCCCCGCTAATAGGTTGGTGTATTCCCCGCCGCATTCGCGGGAAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGatcatc < 3:154642/147‑1 (MQ=255) aaGTCGATATC‑CTGTGGGAGCGGGCTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGTCGTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGa < 1:267336/142‑1 (MQ=255) ccagaTC‑CTGTGGGTGGGGGCTGGCCCGCTAATACGAGGGTGTATTCACCGCCGCATTCGCGGGAAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCg < 3:211974/147‑1 (MQ=255) tC‑CTGTGGGAGCGGGCTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATTCGCGGGAAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGc < 1:40601/149‑1 (MQ=255) tatgtgAGGGGGCTTGCCGGCTAATACGTTGGTGTATTCACCGCGGCATTCGGGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCTAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGt < 5:3889/144‑1 (MQ=255) gCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACg < 5:163329/149‑1 (MQ=255) cGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGaa < 7:165833/95‑1 (MQ=255) cGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGaa > 8:165833/1‑95 (MQ=255) gggCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGcc > 6:290946/1‑149 (MQ=255) ggCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGccc > 3:124426/1‑149 (MQ=255) ggCTTGCCCGCGAATACGATGGTGGATTCACCGACGCATTCGCGGGCAAGCCCACTCCCACATT‑GTACGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATCAAAGTTTTGATNATCGGCAGCGGTGGCAGTGAGCACGca > 4:114544/1‑147 (MQ=255) cTTGCCCGCTAATACGATGGTGGATTCACCGCCGCATGCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctg < 7:64518/149‑1 (MQ=255) ttGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctgg > 8:177285/1‑149 (MQ=255) ccGCGAATGCGGCGGTGAATCCACCATCGTATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctg > 6:94631/1‑149 (MQ=37) ccGCGAATACCATGGTGGATTCACCCCCGCATTCGCGGGCAAGCCCGCTCCCACACT‑GTTCGGCGCAACCCACGGGAACACTTGAATCCAGGTTTTGACAGGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcaag > 7:236610/1‑146 (MQ=255) cgcgAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCcctggcctgg < 2:276693/149‑1 (MQ=255) aaTACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAg > 1:18316/1‑149 (MQ=255) gATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGcc > 3:175247/1‑149 (MQ=255) gATGGTGGATTCACAGCCGCATTCGCGGTCAAGCCAGCTCCCACAGT‑GTTCGGCCCAAGCCCAGGGATCAATTGAATCAAGTTTTTGACATGAAAGTTTTGATCATCGGCAGCGCTGGGCGTGAGCACGCCCTGCACTGGAAACTCTcc > 6:263075/1‑149 (MQ=255) gtggATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAg > 2:110692/1‑148 (MQ=255) gtggATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAg > 2:106706/1‑148 (MQ=255) gtggATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAACTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCAGGAAAGTCGCCCAg > 6:168987/1‑148 (MQ=255) ggATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGAc > 5:37760/1‑149 (MQ=255) gATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGACGCCCAGGAcc > 3:92950/1‑149 (MQ=255) aCCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTg < 3:289841/105‑1 (MQ=255) aCCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTg > 4:289841/1‑105 (MQ=255) aCCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCAcgc > 8:41950/1‑149 (MQ=255) aCCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCAcc < 5:263075/148‑2 (MQ=255) ccgccgCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGACCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCAcgcg > 8:116327/1‑149 (MQ=255) cgccgcATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCACGCGt > 3:113172/1‑149 (MQ=255) cgccgcATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTGTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCATGGCCTGGAAAGTCGCACAGGACCCACGGGt > 7:141740/1‑149 (MQ=255) ccgcATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCACGCGTc > 3:87882/1‑148 (MQ=255) gcATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCAc < 7:34498/141‑1 (MQ=255) gcATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCAc > 8:34498/1‑141 (MQ=255) cATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCACGCGTCgaga < 3:92164/149‑1 (MQ=255) | ATCGCTGCTGCCGACGAGGCCGGCATCGCGATGGTCTTCACTGGCAT‑GCGCCACTTCCGCCACTAATTACGCGGATCCCGAGGCAAGTCGAGATC‑CTGTGGGAGCGGGCTTGCCCGCGAATACGATGGTGGATTCACCGCCGCATTCGCGGGCAAGCCCGCTCCCACAGT‑GTTCGGCGCAAGCCACGGGATCACTTGAATCGAGGTTTTGACATGAAAGTTTTGATCATCGGCAGCGGTGGCCGTGAGCACGCCCTGGCCTGGAAAGTCGCCCAGGACCCACGCGTCGAGA > NC_002947/5485029‑5485319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |