Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,498,833 G→T 6.9% L234L (CTG→CTT) ‡ cbiD → cobalt‑precorrin‑6A synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,498,8330GT 6.9% 174.3 / 3.4 58L234L (CTG→CTT) ‡cbiDcobalt‑precorrin‑6A synthase
Reads supporting (aligned to +/- strand):  ref base G (24/30);  new base T (2/2);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

CTGCGCGGCCTACATTGCCTCGATCCACCAAGGCATCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCGGGCACATGGACCTGCACAGCCGTCACTCCA  >  NC_002947/5498695‑5498972
                                                                                                                                          |                                                                                                                                           
cTGCGCGGCCTACATTGCCTCGATCCACCAAGGCATCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATgg                                                                                                                                   <  3:9967/149‑1 (MQ=255)
   cgcgGCCTACATTGCCTCGATCCACCAAGGCATCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGc                                                                                                                                 <  5:80891/148‑1 (MQ=255)
       gCCTACATTGCCTCGATCCACCAAGGCATCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGAttt                                                                                                                            >  6:254590/1‑149 (MQ=255)
            cATTGCCTCGATCCACCAAGGCATCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTtcgtcg                                                                                                                       <  8:110556/149‑1 (MQ=255)
            cATTGCCTCGATCCACCAAGGCATCGACGTGGCCCACACCAATGGCTACACGCACAAAGCCGCGTGCACCGGCAATGCCAGCGAACACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTtcgtc                                                                                                                        <  3:172871/148‑1 (MQ=255)
                 ccTCGATCCACCAAGGCATCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGcgcg                                                                                                                  >  5:330939/1‑149 (MQ=255)
                 ccTCGATCCACCAAGGCATCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGcgcg                                                                                                                  >  3:249101/1‑149 (MQ=255)
                  cTCGATCCACCAAGGCATCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGTAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCgg                                                                                                                 >  1:109498/1‑149 (MQ=255)
                     gATCCACCAAGGCATCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGc                                                                                                              <  8:279131/149‑1 (MQ=255)
                        ccaccaAGGCATCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGACCTGATCGAAATGGGCGATTTCGTAGGCGCGGTGCTc                                                                                                            >  7:178645/1‑148 (MQ=255)
                          accaAGGCATCGACGTGGCCCACACCAATGGCTTCACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCATTTCGATCAGGGCGATTtc                                                                                                                           >  8:81938/1‑131 (MQ=255)
                          accaAGGCATCGACGTGGCCCACACCAATGGCTTCACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCATTTCGATCAGGGCGATTtc                                                                                                                           <  7:81938/131‑1 (MQ=255)
                                gCATCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTg                                                                                                   <  5:298098/149‑1 (MQ=255)
                                  aTCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAACACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTgcg                                                                                                 >  5:161427/1‑149 (MQ=255)
                                   tCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTgcgc                                                                                                <  5:216358/149‑1 (MQ=255)
                                   tCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTgcg                                                                                                 >  3:2596/1‑148 (MQ=255)
                                      aCGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAg                                                                                             >  6:260925/1‑149 (MQ=255)
                                             ccACACCAATGGCAACACGCACATAGCCGCCTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGCCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCCCAACGTACCgg                                                                                      >  8:185754/1‑149 (MQ=255)
                                                cacCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGc                                                                                   <  4:10256/149‑1 (MQ=255)
                                                  ccAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCcg                                                                                 >  1:58978/1‑149 (MQ=255)
                                                         cTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGa                                                                          <  1:222692/149‑1 (MQ=255)
                                                             acGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGAcc                                                                        >  2:323170/1‑147 (MQ=255)
                                                             acGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGAcc                                                                        <  1:323170/147‑1 (MQ=255)
                                                             acGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCt                                                                      <  4:249101/149‑1 (MQ=255)
                                                               gCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCtgt                                                                    >  6:154697/1‑149 (MQ=255)
                                                               gCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCtgt                                                                    >  1:228235/1‑149 (MQ=255)
                                                                 acaTAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCGCCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTgc                                                                  >  5:181505/1‑149 (MQ=255)
                                                                    tAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTgcggc                                                               <  5:154697/149‑1 (MQ=255)
                                                                         gcgTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCtt                                                          >  4:247785/1‑149 (MQ=255)
                                                                          cgTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTc                                                         <  4:2596/149‑1 (MQ=255)
                                                                          cgTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTc                                                         <  3:44054/149‑1 (MQ=255)
                                                                           gTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCGCCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCg                                                        <  6:181505/149‑1 (MQ=255)
                                                                               aCCGGCAATGCCTGCGAAGACTCCATGCGCCGCGTCTACGGTCTGCCGGACATCGCCCTGATCGAAATGGGCGATTTCGTCGGCTCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCaa                                                    <  7:185754/149‑1 (MQ=255)
                                                                               aCCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCaa                                                                                              >  4:216945/1‑107 (MQ=255)
                                                                               aCCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCaa                                                                                              <  3:216945/107‑1 (MQ=255)
                                                                               aCCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGc                                                      >  3:242265/1‑147 (MQ=255)
                                                                                   gCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGAt                                                 >  5:16072/1‑148 (MQ=255)
                                                                                    cAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCa                                               <  2:58978/149‑1 (MQ=255)
                                                                                    cAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCa                                               <  6:179795/149‑1 (MQ=255)
                                                                                         ccAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCaaa                                          <  5:254590/149‑1 (MQ=255)
                                                                                          cAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAAc                                         >  5:148297/1‑149 (MQ=255)
                                                                                                    acCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTgg                                      <  3:283082/142‑1 (MQ=255)
                                                                                                    acCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCgg                               >  7:283446/1‑149 (MQ=255)
                                                                                                      cATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGa                                                  <  5:7860/128‑1 (MQ=255)
                                                                                                      cATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGa                                                  >  6:7860/1‑128 (MQ=255)
                                                                                                      cATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCggg                              >  2:268882/1‑148 (MQ=255)
                                                                                                          cgccgcGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCGGGcaca                          <  4:242265/148‑1 (MQ=255)
                                                                                                            ccgcGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCg                                                                                       >  4:243224/1‑85 (MQ=255)
                                                                                                            ccgcGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCg                                                                                       <  3:243224/85‑1 (MQ=255)
                                                                                                              gcgTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCGGGCACATGGAc                     <  6:161427/149‑1 (MQ=255)
                                                                                                                gTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCGGGCACATGGACCt                   <  5:260925/149‑1 (MQ=255)
                                                                                                                      ggTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCGGGCACATGGACCTGCACAg             <  6:330939/149‑1 (MQ=255)
                                                                                                                             acgAAATCGCCCATTTCGATCAGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCGGGCACATGGACCTGCACAGCCGTc        <  2:10763/145‑1 (MQ=255)
                                                                                                                             acgAAATCGCCCATTTCGATCAGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCGGGCACATGGACCTGCACAGCCGTc        >  1:10763/3‑147 (MQ=255)
                                                                                                                              ggAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCGGGCACATGGACCTGCACAGCCGTCACt     >  1:302886/1‑149 (MQ=255)
                                                                                                                                 aaTCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCGGGCACATGGACCTGCACAGCCGTCACTCCa  <  2:228235/149‑1 (MQ=255)
                                                                                                                                 aaTCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCACCGGGCACATGGACCTGCACAGCCGTCACTCCa  <  6:16072/149‑1 (MQ=255)
                                                                                                                                 aaTCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCATGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCGGGCACATGGACCTGCACAGCCGTCACTCCa  <  2:109498/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CTGCGCGGCCTACATTGCCTCGATCCACCAAGGCATCGACGTGGCCCACACCAATGGCTACACGCACATAGCCGCGTGCACCGGCAATGCCAGCGAAGACACCATGCGCCGCGTCTACGGTCTGCCGGAAATCGCCCTGATCGAAATGGGCGATTTCGTCGGCGCGGTGCTCAAGCACCTGCGCAAGGTACCGGTGCCGCGCCTGACCCTGTGCGGCGGCTTCGGCAAGATCAGCAAACTGGCCGCCGGGCACATGGACCTGCACAGCCGTCACTCCA  >  NC_002947/5498695‑5498972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑