Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,529,360 C→G 14.8% intergenic (‑574/+20) ydfG ← / ← livF‑II bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/malonic semialdehyde reductase/ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,529,3600CG14.8% 118.5 / 11.3 47intergenic (‑574/+20)ydfG/livF‑IIbifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/malonic semialdehyde reductase/ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (23/17);  new base G (5/2);  total (28/19)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01

TGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGACAGTTTCAGCGCATGGTTGGC  >  NC_002947/5529221‑5529508
                                                                                                                                           |                                                                                                                                                    
tGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCAGTGCAGcccc                                                                                                                                             <  2:119996/149‑1 (MQ=255)
     ccaTGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTaa                                                                                                                                         >  4:80674/1‑148 (MQ=255)
                           cacaCTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGAt                                                                                                                  <  8:1273/149‑1 (MQ=255)
                                     gTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAg                                                                                                               <  7:301333/142‑1 (MQ=255)
                                     gTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAg                                                                                                               >  8:301333/1‑142 (MQ=255)
                                         aaaaGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCACTTAAAACAACTAAGTTCCCGCCCAGATAAGCGTTAAGCAc                                                                                                     >  4:154787/1‑148 (MQ=255)
                                          aaaGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACtt                                                                                                   <  2:66971/149‑1 (MQ=255)
                                           aaGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTc                                                                                                  >  7:5137/1‑149 (MQ=255)
                                              agTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCt                                                                                                >  1:218311/1‑148 (MQ=255)
                                              agTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCt                                                                                                <  2:218311/148‑1 (MQ=255)
                                                     cTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCAt                                                                                             >  7:105469/1‑144 (MQ=255)
                                                     cTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCAt                                                                                             <  8:105469/144‑1 (MQ=255)
                                                     cTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTACCACATTGAGAAGGGGGCTGCACTGCAGCCCCGTAAAACAACTAAGTGCCCGACCAGATAAGCGTTAAGCACATCCTCAATGAc                                                                                         >  2:158990/1‑148 (MQ=255)
                                                           aaaCAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTa                                                                                                          >  2:74586/1‑125 (MQ=255)
                                                           aaaCAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTa                                                                                                          <  1:74586/125‑1 (MQ=255)
                                                               aaTGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCaa                                                                                      <  5:214931/141‑1 (MQ=255)
                                                               aaTGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCaa                                                                                      >  6:214931/1‑141 (MQ=255)
                                                                   gCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGcc                                                                           <  6:217818/148‑1 (MQ=255)
                                                                        cGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGcccggtc                                                                      <  3:94087/148‑1 (MQ=255)
                                                                         gAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGcccggtccc                                                                    >  2:111012/1‑149 (MQ=255)
                                                                         gAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGcccggtccc                                                                    >  5:116842/1‑149 (MQ=255)
                                                                         gAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGcccggtccc                                                                    >  3:291781/1‑149 (MQ=255)
                                                                             gAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTTCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACGTCCTCATTGACCAACAGTTCCTGcccggtcccggt                                                                 >  2:253497/1‑148 (MQ=255)
                                                                                   gggTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCg                                                           >  8:248642/1‑148 (MQ=255)
                                                                                    ggTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGaa                                                         >  1:33264/1‑149 (MQ=255)
                                                                                          tttCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGcc                                                   <  6:116842/149‑1 (MQ=255)
                                                                                          tttCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGcc                                                   <  4:291781/149‑1 (MQ=255)
                                                                                          tttCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGcc                                                   >  3:141744/1‑149 (MQ=255)
                                                                                          tttCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGc                                                    <  8:5137/148‑1 (MQ=255)
                                                                                                     gggAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCAt                                         >  6:2164/1‑148 (MQ=255)
                                                                                                         aCGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAAt                                                        >  3:91506/1‑129 (MQ=255)
                                                                                                         aCGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACAt                                    <  3:154787/149‑1 (MQ=255)
                                                                                                          cGACAAGTGTTCCACATTGAGACCGGTGCTGCCCGGCAGCCCCTTAACACAACTCCGTGCCCGCCCAGATAAGCGTTTCGCACTTCCTCATTGACCAACAGTTGCTGGCCGGTCCCGGTCAAGCGAAGCTGCCCGTTTACCATCACAtc                                   <  4:241342/149‑2 (MQ=255)
                                                                                                           gACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGTCCGTTGACCATcac                                      <  7:292155/145‑1 (MQ=255)
                                                                                                           gACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTACTGCCCGGTCCCGGTCATTCGAATCTGCCCGTTGACCATcacaag                                   >  8:292155/1‑146 (MQ=255)
                                                                                                              aaGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTACCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGcc                               <  3:80674/149‑1 (MQ=255)
                                                                                                                   ttCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTc                          >  1:122327/1‑149 (MQ=255)
                                                                                                                      cacaTTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGa                       >  5:66256/1‑149 (MQ=255)
                                                                                                                        caTTGAGAAGGGGGCTGCACTGCAGCCCGTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGACa                     <  1:158990/149‑1 (MQ=255)
                                                                                                                             gttaaggggCTGCAGTGCAGCCCCCTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAg                                  <  8:283340/126‑1 (MQ=255)
                                                                                                                             gttaaggggCTGCAGTGCAGCCCCCTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAg                                  >  7:283340/6‑131 (MQ=255)
                                                                                                                                 aggggCTGCAGTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGACAGTTTCAgcg            >  3:252699/2‑149 (MQ=255)
                                                                                                                                  ggggCTGCAGTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGACAGTTTCAgcg            >  2:311744/1‑148 (MQ=255)
                                                                                                                                  ggggCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGACAGTTTCAgcg            >  8:63656/1‑148 (MQ=255)
                                                                                                                                  ggggCTCCAGTGCAGCAACTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCAATGACCAACAGTTCCAGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACAATCACATAGCCCCGCTCAGACACTTTCAgcg            >  4:202475/1‑148 (MQ=255)
                                                                                                                                    ggCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGACAGTTTCAGCGCAt         >  3:334742/1‑149 (MQ=255)
                                                                                                                                     gCTGCAGTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGACAGTTTCAGCGCATg        >  1:206786/1‑149 (MQ=255)
                                                                                                                                       tgcactgcaGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGACAGTTTCAGCGCATGGt      >  5:3468/1‑149 (MQ=255)
                                                                                                                                         cactgcaGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGACAGTTTCAGCGCATGGTTg    <  6:66256/149‑1 (MQ=255)
                                                                                                                                          actgcaGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCg                             <  2:92799/123‑1 (MQ=255)
                                                                                                                                          actgcaGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCg                             >  1:92799/1‑123 (MQ=255)
                                                                                                                                           ctgcaGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGACAGTTTCAGCGCATGGTTGGc  <  5:2164/149‑1 (MQ=255)
                                                                                                                                           ctgcaGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGACAGTTTCAGCGCATGGTTGGc  >  2:268657/1‑149 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
TGCCACCATGTTTTCCACAGGCGGCTCCACACTATCCGTGGAAAAGAGTTGGCCTGTGGAAACAATGGCTTGCGAAGGAATATGGGTGTGTTTCGGGAGAAGGGAACGACAAGTTTTCCACATTGAGAAGGGGGCTGCACTGCAGCCCCTTAACACAACTCAGTGCCCGCCCAGATAAGCGTTACGCACTTCCTCATTGACCAACAGTTCCTGCCCGGTCCCGGTCATGCGAATCTGCCCGTTGACCATCACATAGGCCCGGTCCGACAGTTTCAGCGCATGGTTGGC  >  NC_002947/5529221‑5529508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑