Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,532,230 T→G 6.2% K15Q (AAG→CAG) ‡ braE ← high‑affinity branched‑chain amino acid ABC transporter permease BraE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,532,2300TG 6.2% 197.7 / 4.4 65K15Q (AAG→CAG) ‡braEhigh‑affinity branched‑chain amino acid ABC transporter permease BraE
Reads supporting (aligned to +/- strand):  ref base T (25/35);  new base G (2/2);  total (28/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

ACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAGGATCATCACCAGCAGCGAGAAGCTGAACACATCCTTGTAGTCGGAGTTGA  >  NC_002947/5532082‑5532372
                                                                                                                                                       |                                                                                                                                              
acacCTCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCtt                                                                                                                                                <  1:307992/149‑1 (MQ=255)
  acCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTgg                                                                                                                                              <  1:153426/149‑1 (MQ=255)
         aCCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGc                                                                                                                                        >  3:61930/1‑148 (MQ=255)
         aCCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCt                                                                                                                                       <  5:286707/149‑1 (MQ=255)
         aCCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCAAGGGTTTCgac                                                                                                                                       <  4:279590/149‑3 (MQ=255)
             gccagGCCACGCGGCGCGGCTCGGCAGTGAAGGTGTAGCCGTCGAGCACCACACCAACGCCCGGACCCACCACGATAAGTGCC‑‑AGCAGGCCA‑GCGCCAATGGTCTCCAGCAGGCTGCGTTTAAGGGCGAAACCCTTGGTTTCGCGaa                                                                                                                                     >  3:211786/1‑147 (MQ=255)
                agGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCg                                                                                                                                         <  4:51495/140‑1 (MQ=255)
                agGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCg                                                                                                                                         >  3:51495/1‑140 (MQ=255)
                 ggCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAgg                                                                                                                               <  5:350086/149‑1 (MQ=255)
                     aCGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGt                                                                                                                           >  5:250953/1‑149 (MQ=255)
                       gcggcgcggcTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGCCAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGttt                                                                                                                         >  3:219378/1‑149 (MQ=255)
                       gcggcgcggcTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGAAAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGATTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGttt                                                                                                                         >  4:21747/1‑149 (MQ=255)
                        cggcgcggcTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATTGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGtttt                                                                                                                        <  2:152702/149‑1 (MQ=255)
                        cggcgcggcTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGtttt                                                                                                                        <  6:250953/149‑1 (MQ=255)
                          gcgcggcTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTgg                                                                                                                      <  6:127018/149‑1 (MQ=255)
                           ggcgggTCGGCATTGAATGGGTATCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCATGCCATGCGTCAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTgg                                                                                                                      <  1:276350/148‑1 (MQ=255)
                              ggcTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAAc                                                                                                                  <  4:43915/149‑1 (MQ=255)
                                 tCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCCGAAACAGGACA‑GCGACAATGGTCACCAGCAGGCTGCGTTTAATGTCAAAACCATTGGTTTCTCTAATAGAGGCAGTTTTGGCAACg                                                                                                                 >  1:275986/1‑149 (MQ=255)
                                 tCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATa                                                                                                                                   >  7:60664/1‑129 (MQ=255)
                                 tCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATa                                                                                                                                   <  8:60664/129‑1 (MQ=255)
                                    gCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACAt                                                                                                            >  2:310895/1‑149 (MQ=255)
                                      aTTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGttt                                                                                                                         <  6:96111/134‑1 (MQ=255)
                                      aTTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGttt                                                                                                                         >  5:96111/1‑134 (MQ=255)
                                         gAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTca                                                                                                       >  7:49113/1‑149 (MQ=255)
                                          aaGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCtt                                                                                                                                                >  3:110653/1‑107 (MQ=255)
                                          aaGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCtt                                                                                                                                                <  4:110653/107‑1 (MQ=255)
                                          aaGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTGGCTAATAGAGGCAGTTTTGGCAACGGACATGTTca                                                                                                       >  2:292901/1‑148 (MQ=255)
                                          aaGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTcac                                                                                                      <  6:222618/149‑1 (MQ=255)
                                          aaGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTca                                                                                                       >  5:222618/1‑148 (MQ=255)
                                           aGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTcaca                                                                                                     <  3:293156/149‑1 (MQ=255)
                                                tAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTa                                                                                                <  4:61930/149‑1 (MQ=255)
                                                  gCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACATCTTAGc                                                                                              <  2:275986/149‑1 (MQ=255)
                                                  gCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCGGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGc                                                                                              <  4:234095/149‑1 (MQ=255)
                                                   ccGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCAAGGGTTTCgac                                                                                                                                       <  7:280630/107‑3 (MQ=255)
                                                   ccGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCAAGGGTTTCgac                                                                                                                                       >  8:280630/1‑105 (MQ=255)
                                                            accacaccaACGACCGTCCCACACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAAGGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGGTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCggg                                                                                    <  4:211786/149‑1 (MQ=255)
                                                              cacaccaACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCAc                                                                                           <  4:320888/140‑1 (MQ=255)
                                                              cacaccaACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCAc                                                                                           >  3:320888/1‑140 (MQ=255)
                                                                caccaACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCTACGAGCGGGCGAc                                                                                >  4:214135/1‑149 (MQ=255)
                                                                      cGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCcagca                                                                          <  4:219378/149‑1 (MQ=255)
                                                                       gACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCcagcag                                                                         <  2:21955/149‑1 (MQ=255)
                                                                       gACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCcagcag                                                                         <  6:261258/149‑1 (MQ=255)
                                                                         ccGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCcag                                                                            >  8:162684/1‑144 (MQ=255)
                                                                         ccGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCcag                                                                            <  7:162684/144‑1 (MQ=255)
                                                                            gACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCtt                                                                    >  7:93647/1‑149 (MQ=255)
                                                                            gACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCtt                                                                    <  8:93647/149‑1 (MQ=255)
                                                                              ccATACACGATAAGTGCC‑‑AGCATGCCA‑TCGACAATGTTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTtgt                                                                  <  4:108401/149‑1 (MQ=255)
                                                                                 aaCACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCcagca                                                                          <  5:156598/138‑1 (MQ=255)
                                                                                 aaCACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCcagca                                                                          >  6:156598/1‑138 (MQ=255)
                                                                                      gATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTg                                                                                                                       <  5:45559/88‑1 (MQ=255)
                                                                                      gATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTg                                                                                                                       >  6:45559/1‑88 (MQ=255)
                                                                                           gTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTtgt                                                                  >  2:204376/1‑136 (MQ=255)
                                                                                           gTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTtgt                                                                  <  1:204376/136‑1 (MQ=255)
                                                                                           gTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGAt                                                       >  6:50798/1‑147 (MQ=255)
                                                                                                   gcagGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAg                                                    <  2:146995/144‑1 (MQ=255)
                                                                                                   gcagGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAg                                                    >  1:146995/1‑144 (MQ=255)
                                                                                                       gCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAGGATCATCAc                                           <  2:31815/149‑1 (MQ=255)
                                                                                                       gCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAGGATCATCAc                                           <  6:175973/149‑1 (MQ=255)
                                                                                                       gCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAGGATCATCAc                                           <  5:50798/149‑1 (MQ=255)
                                                                                                          a‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAGGATCATCACcag                                        >  3:79903/1‑149 (MQ=255)
                                                                                                               aCAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAGGATCATCACCagcagc                                    <  1:310895/149‑1 (MQ=255)
                                                                                                                 aaTGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAGGATCATCACCAGCAGCga                                  <  4:79903/149‑1 (MQ=255)
                                                                                                                              agGCTGCGTTTAAGGTCGAAACCCTCGGTTTCGCTAATAGAGGAAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAGGATCATCACCAGCAGCGAGAAGCTGAAcaca                     >  8:170293/1‑149 (MQ=255)
                                                                                                                                gCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTgg                                                                >  1:160729/1‑104 (MQ=255)
                                                                                                                                gCTGCGTTTAAGCTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTgg                                                                <  2:160729/104‑1 (MQ=255)
                                                                                                                                    cGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAGGATCATCACCAGCAGCGAGAAGCTGAACACATCCTTg               <  3:214135/149‑1 (MQ=255)
                                                                                                                                             gcGAAACCAAGGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAGGATCATCACCAGCAGCGAGAAGCTGAACACATCCTTGTAGTCGGAg      >  1:49947/2‑149 (MQ=255)
                                                                                                                                                 aaCCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAGGATCATCACCAGCAGCGAGAAGCTGAACACATCCTTGTAGTCGGAGTTGa  >  5:2385/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                              
ACACCGCCGACCAGCCAGGCCACGCGGCGCGGCTCGGCATTGAAGGTGTAGCCGTCGAGCACCACACCAACGACCGGACCAAACACGATAAGTGCC‑‑AGCAGGCCA‑GCGACAATGGTCTCCAGCAGGCTGCGTTTAAGGTCGAAACCCTTGGTTTCGCTAATAGAGGCAGTTTTGGCAACGGACATGTTCACACCTTAGCCACGAGCGGGCGACCCAGCAGGCCTTGTGGGCGGAAGATAAGGATCATCACCAGCAGCGAGAAGCTGAACACATCCTTGTAGTCGGAGTTGA  >  NC_002947/5532082‑5532372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑