Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,537,067 A→G 10.6% intergenic (+44/+59) nadE → / ← PP_4870 NH(3)‑dependent NAD(+) synthetase/azurin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,537,0670AG10.6% 118.1 / 4.3 49intergenic (+44/+59)nadE/PP_4870NH(3)‑dependent NAD(+) synthetase/azurin
Reads supporting (aligned to +/- strand):  ref base A (19/23);  new base G (3/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

ATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGG  >  NC_002947/5536920‑5537214
                                                                                                                                                   |                                                                                                                                                   
aTGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGaa                                                                                                                                                    <  6:25180/149‑1 (MQ=255)
   ccTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGGTAACCCGCGCAAACGAGGGTGAAacc                                                                                                                                                 >  6:185957/1‑149 (MQ=255)
    cTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAAcaca                                                                                                                                                >  2:42184/1‑149 (MQ=255)
       ccTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCAcc                                                                                                                                              <  1:154220/148‑1 (MQ=255)
            aTGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCTCGTATTCGCGGGTGAAcccgc                                                                                                                                               <  1:201565/142‑3 (MQ=255)
            aTGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCTCGTATTCGCGGGTGAAcccgc                                                                                                                                               >  2:201565/1‑140 (MQ=255)
             tGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCAt                                                                                                                                       >  3:197814/1‑149 (MQ=255)
                  aGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgcg                                                                                                                                  <  4:197814/149‑1 (MQ=255)
                   gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                 <  5:203775/149‑1 (MQ=25)
                   gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                                 <  7:299405/149‑1 (MQ=255)
                   gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                                 >  2:11411/1‑149 (MQ=255)
                       gTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCCGGAGCGGGTTGTCCCGCGAAGACGAGGGGGGCACCACCGACGCATTCGCggggg                                                                                                                              >  8:235645/1‑146 (MQ=255)
                        ttGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGaa                                                                                                                            <  1:11411/149‑1 (MQ=255)
                             cAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAAcc                                                                                                                                                  >  2:232707/1‑122 (MQ=255)
                             cAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAAcc                                                                                                                                                  <  1:232707/122‑1 (MQ=255)
                             cAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACCCAGTAGCGGGTGAACCCGc                                                                                                                       >  5:127977/1‑149 (MQ=255)
                               ggCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCCGCACAAGCGCGACCTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGGGAACCCACCGACGCATTCGCGGGTGAACCCGCTc                                                                                                                     <  3:328988/149‑1 (MQ=255)
                               ggCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTc                                                                                                                     <  8:251841/149‑1 (MQ=255)
                                gCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCCCCGCATTCGCGGGTGGACCCGCtgc                                                                                                                    >  7:78211/1‑147 (MQ=255)
                                 cAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGCATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCt                                                                                                                   >  1:5723/1‑149 (MQ=255)
                                     gTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAg                                                                                                               >  1:32916/1‑149 (MQ=255)
                                        cGACATCATCGGCGCCACCAACCCCAAGCCCCAGCACAAGCCCGAACTGCCGTTCGCTCCTTGATAAGGGGCCTGCAGGCGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGCATGCGCGGGTGAACCCGCTCCTGCAggg                                                                                                             <  7:235645/148‑1 (MQ=38)
                                           catcatGGTGGACAACTACCTCATCACCCAGAACTAGCTCCAACTGGCGTTGGATCTTTGATATGGGTGCTGCTTGTTGGGGTTCCACGGCTATTACGAGGTTTAACCCACCGTGTCATTCGCGGTTTAACCCGCTCCTGCAGGGTTCg                                                                                                         <  8:73342/149‑1 (MQ=255)
                                           attcatcGTCGCCACCTAGCGCAAGACCCTGCACAAGGGCGAACTGCCTTGCGGTCCTTTATAAGTGGCCTGCAGGTGGGGGTTCACCGGCTAATAGGTGGGTGAACCCACCGACGCTTTGGCGGGTGAACCCGCTCCTGCAGGGTTc                                                                                                          <  8:78211/146‑1 (MQ=255)
                                              catcGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                 >  1:121689/1‑122 (MQ=18)
                                              catcGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                 <  2:121689/122‑1 (MQ=18)
                                              catcGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGAATACGAGGGTGAACCCCCCGACGCATTCGCGGGTGGACCCGCGCCTGGAGGGTTAGCCg                                                                                                      >  3:94262/1‑149 (MQ=255)
                                              catcGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAAACCCCGAATACGAGGGTTCAACCCCCCACGCCTTAGCGGGTGAACCCCCAACAGCACGGTTGGCCg                                                                                                      >  8:199883/1‑149 (MQ=255)
                                                tcgtcgCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTACGGCAGGGGTCGCCGt                                                                                                     >  2:230132/1‑148 (MQ=255)
                                                tcgtcgCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAAACCCCCAAAACCACGGTGAACCCACCCACCCATTCGCGGGTGTACACCCGCCAGCACGGTTAGCCGt                                                                                                     >  7:197465/1‑148 (MQ=255)
                                                   tcgCCACATACCGCAAGACCCAGCACACGCGCTAACTGCCGTTCGCTCTTTTATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGTGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGc                                                                                                 <  4:94262/149‑1 (MQ=38)
                                                       caacTACCGCAAGACCCTCCAAATGGGCTAAGTGCCGTTCGCTCGTTTATATGTGGCCTGCTGTTGGGGGTTCACCCGCTAAAACTAGGTTTAACCCACCGACGCATTGGCGGGTTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCttt                                                                                             <  6:127977/146‑1 (MQ=255)
                                                        aCCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATACGTGGCCTCCAGGCGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTg                                                                                            <  5:185957/149‑1 (MQ=255)
                                                                aaaGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTg                                                                                    <  7:199883/148‑1 (MQ=255)
                                                                      ccAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGGGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGa                                                                                   <  5:261559/144‑1 (MQ=255)
                                                                            aaggagcgAACGGCAGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGAATACGAGGGTGAACCCACCCCCCCATTCGCGGGTGAACCCCCTCCTGGAGGGTTCGCCGTCAGCCGTTGATTACTTTACGAAGACCATg                                                                        >  8:91713/6‑149 (MQ=255)
                                                                               gcgcGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCt                                                                     <  2:32916/149‑1 (MQ=255)
                                                                                 gcgAACGGCAGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGACCCCACCGACGCCTTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGTTTACTTGACGATGACCTTGCCttt                                                                   >  5:245634/1‑149 (MQ=38)
                                                                                                   cTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTgg                                                   <  1:230132/147‑1 (MQ=255)
                                                                                                            tatccTCCAGGAGCGGGTTCACCCGCGAATACGCGGTTTTACCCACGTTCCCATGCGCGGGCTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTCCCTTTCATCATCGAGATGTGGCCCGGGAAg                                          <  1:29409/144‑1 (MQ=255)
                                                                                                                           ggTTCACCCGCTAATACGAGGGGGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTa                         <  2:5723/149‑1 (MQ=255)
                                                                                                                           ggTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTtc                                                                  <  7:167795/108‑1 (MQ=255)
                                                                                                                           ggTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTtc                                                                  >  8:167795/1‑108 (MQ=255)
                                                                                                                             ttCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAg                        >  4:83971/1‑148 (MQ=255)
                                                                                                                             ttCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGt                       <  3:83971/149‑1 (MQ=255)
                                                                                                                               cACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTca                     >  8:231817/1‑149 (MQ=25)
                                                                                                                                 cccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcac                    >  1:342184/1‑148 (MQ=255)
                                                                                                                                    gcgAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCAGCTCCTGCAGGGTGCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCaccacc                >  8:132593/1‑149 (MQ=255)
                                                                                                                                           cGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTcc         <  2:342184/149‑1 (MQ=255)
                                                                                                                                                gTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCtt    <  5:311197/149‑1 (MQ=255)
                                                                                                                                                   aaCCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTgg  >  7:286631/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATGCCTTCCTGCATGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGG  >  NC_002947/5536920‑5537214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑