Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,537,079 C→T 8.9% intergenic (+56/+47) nadE → / ← PP_4870 NH(3)‑dependent NAD(+) synthetase/azurin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,537,0790CT 8.9% 114.2 / 2.4 45intergenic (+56/+47)nadE/PP_4870NH(3)‑dependent NAD(+) synthetase/azurin
Reads supporting (aligned to +/- strand):  ref base C (19/22);  new base T (2/2);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.96e-01

TGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTC  >  NC_002947/5536933‑5537221
                                                                                                                                                  |                                                                                                                                              
tGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCAt                                                                                                                                              >  3:197814/1‑149 (MQ=255)
     aGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTcgcg                                                                                                                                         <  4:197814/149‑1 (MQ=255)
      gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                        <  5:203775/149‑1 (MQ=25)
      gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                                        >  2:11411/1‑149 (MQ=255)
      gCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCgg                                                                                                                                        <  7:299405/149‑1 (MQ=255)
          gTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCCGGAGCGGGTTGTCCCGCGAAGACGAGGGGGGCACCACCGACGCATTCGCggggg                                                                                                                                     >  8:235645/1‑146 (MQ=255)
           ttGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGaa                                                                                                                                   <  1:11411/149‑1 (MQ=255)
                cAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACCCAGTAGCGGGTGAACCCGc                                                                                                                              >  5:127977/1‑149 (MQ=255)
                  ggCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCCGCACAAGCGCGACCTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGGGAACCCACCGACGCATTCGCGGGTGAACCCGCTc                                                                                                                            <  3:328988/149‑1 (MQ=255)
                  ggCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTc                                                                                                                            <  8:251841/149‑1 (MQ=255)
                   gCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCCCCGCATTCGCGGGTGGACCCGCtgc                                                                                                                           >  7:78211/1‑147 (MQ=255)
                    cAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGCATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCt                                                                                                                          >  1:5723/1‑149 (MQ=255)
                        gTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAg                                                                                                                      >  1:32916/1‑149 (MQ=255)
                           cGACATCATCGGCGCCACCAACCCCAAGCCCCAGCACAAGCCCGAACTGCCGTTCGCTCCTTGATAAGGGGCCTGCAGGCGCGGGTTCACCCGCTAATACGAGGGTGAACCCACCGACGCATGCGCGGGTGAACCCGCTCCTGCAggg                                                                                                                    <  7:235645/148‑1 (MQ=38)
                              catcatGGTGGACAACTACCTCATCACCCAGAACTAGCTCCAACTGGCGTTGGATCTTTGATATGGGTGCTGCTTGTTGGGGTTCCACGGCTATTACGAGGTTTAACCCACCGTGTCATTCGCGGTTTAACCCGCTCCTGCAGGGTTCg                                                                                                                <  8:73342/149‑1 (MQ=255)
                              attcatcGTCGCCACCTAGCGCAAGACCCTGCACAAGGGCGAACTGCCTTGCGGTCCTTTATAAGTGGCCTGCAGGTGGGGGTTCACCGGCTAATAGGTGGGTGAACCCACCGACGCTTTGGCGGGTGAACCCGCTCCTGCAGGGTTc                                                                                                                 <  8:78211/146‑1 (MQ=255)
                                 catcGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                        >  1:121689/1‑122 (MQ=18)
                                 catcGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCgg                                                                                                                                        <  2:121689/122‑1 (MQ=18)
                                 catcGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGAATACGAGGGTGAACCCCCCGACGCATTCGCGGGTGGACCCGCGCCTGGAGGGTTAGCCg                                                                                                             >  3:94262/1‑149 (MQ=255)
                                 catcGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAAACCCCGAATACGAGGGTTCAACCCCCCACGCCTTAGCGGGTGAACCCCCAACAGCACGGTTGGCCg                                                                                                             >  8:199883/1‑149 (MQ=255)
                                   tcgtcgCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTACGGCAGGGGTCGCCGt                                                                                                            >  2:230132/1‑148 (MQ=255)
                                   tcgtcgCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAAACCCCCAAAACCACGGTGAACCCACCCACCCATTCGCGGGTGTACACCCGCCAGCACGGTTAGCCGt                                                                                                            >  7:197465/1‑148 (MQ=255)
                                      tcgCCACATACCGCAAGACCCAGCACACGCGCTAACTGCCGTTCGCTCTTTTATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGTGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGc                                                                                                        <  4:94262/149‑1 (MQ=38)
                                          caacTACCGCAAGACCCTCCAAATGGGCTAAGTGCCGTTCGCTCGTTTATATGTGGCCTGCTGTTGGGGGTTCACCCGCTAAAACTAGGTTTAACCCACCGACGCATTGGCGGGTTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCttt                                                                                                    <  6:127977/146‑1 (MQ=255)
                                           aCCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATACGTGGCCTCCAGGCGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTg                                                                                                   <  5:185957/149‑1 (MQ=255)
                                                   aaaGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTg                                                                                           <  7:199883/148‑1 (MQ=255)
                                                         ccAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGGGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGa                                                                                          <  5:261559/144‑1 (MQ=255)
                                                               aaggagcgAACGGCAGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTAACCCGCGAATACGAGGGTGAACCCACCCCCCCATTCGCGGGTGAACCCCCTCCTGGAGGGTTCGCCGTCAGCCGTTGATTACTTTACGAAGACCATg                                                                               >  8:91713/6‑149 (MQ=255)
                                                                  gcgcGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCt                                                                            <  2:32916/149‑1 (MQ=255)
                                                                    gcgAACGGCAGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGACCCCACCGACGCCTTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGTTTACTTGACGATGACCTTGCCttt                                                                          >  5:245634/1‑149 (MQ=38)
                                                                                      cTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTgg                                                          <  1:230132/147‑1 (MQ=255)
                                                                                               tatccTCCAGGAGCGGGTTCACCCGCGAATACGCGGTTTTACCCACGTTCCCATGCGCGGGCTAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTCCCTTTCATCATCGAGATGTGGCCCGGGAAg                                                 <  1:29409/144‑1 (MQ=255)
                                                                                                              ggTTCACCCGCTAATACGAGGGGGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTa                                <  2:5723/149‑1 (MQ=255)
                                                                                                              ggTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTtc                                                                         <  7:167795/108‑1 (MQ=255)
                                                                                                              ggTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTtc                                                                         >  8:167795/1‑108 (MQ=255)
                                                                                                                ttCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAg                               >  4:83971/1‑148 (MQ=255)
                                                                                                                ttCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGt                              <  3:83971/149‑1 (MQ=255)
                                                                                                                  cACCCGCGAATGCGTCGGTGGGTTCACCCTCGTATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTca                            >  8:231817/1‑149 (MQ=25)
                                                                                                                    cccGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTcac                           >  1:342184/1‑148 (MQ=255)
                                                                                                                       gcgAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCAGCTCCTGCAGGGTGCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCaccacc                       >  8:132593/1‑149 (MQ=255)
                                                                                                                              cGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTcc                <  2:342184/149‑1 (MQ=255)
                                                                                                                                   gTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCtt           <  5:311197/149‑1 (MQ=255)
                                                                                                                                      aaCCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTgg         >  7:286631/1‑148 (MQ=255)
                                                                                                                                       aCCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATGATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGaa       <  1:126224/149‑1 (MQ=255)
                                                                                                                                             cGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTc  >  6:281702/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
TGGGCAGCCGGTTGACCAGGCAGCGTTCGACATCATCGTCGCCACCTACCGCAAGACCCAGCACAAGCGCGAACTGCCGTTCGCTCCTTGATAAGTGGCCTGCAGGAGCGGGTTCACCCGCGAATACGAGGGTGAACCCACCGACGCATTCGCGGGTGAACCCGCTCCTGCAGGGTTCGCCGTCAGCCTTTGATTACTTGACGATGACCTTGCCTTTCATCATCGAGATGTGGCCCGGGAAGGTGCAGAAGAAGCTGTAGTCACCACCGGCTTCCAGCTTGGAAGTGTC  >  NC_002947/5536933‑5537221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑