Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,550,661 T→A 10.4% S301S (TCT→TCA)  PP_4881 → iron ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,550,6610TA10.4% 129.6 / 5.0 48S301S (TCT→TCA) PP_4881iron ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (22/21);  new base A (2/3);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GTCACTATCTCCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTAC  >  NC_002947/5550515‑5550805
                                                                                                                                                  |                                                                                                                                                
gTCACTATCTCCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCt                                                                                                                                                  <  8:211690/147‑1 (MQ=255)
 tCACTATCTCCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGAc                                                                                                                                               <  1:93841/149‑1 (MQ=255)
   aCTATCTCCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGAcc                                                                                                                                              <  8:121639/148‑1 (MQ=255)
   aCTATCTCCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACcg                                                                                                                                             >  5:119453/1‑149 (MQ=255)
         tcCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCt                                                                                                                                       >  1:205088/1‑149 (MQ=255)
          ccGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGc                                                                                                                                         <  6:79340/146‑1 (MQ=255)
          ccGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGc                                                                                                                                         >  5:79340/1‑146 (MQ=255)
           cGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCATCTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGAGCACCGCCGAGGAGCCACTGCACAAGGGCTTGGTTTCTGACCGCGGcc                                                                                                                                        <  5:155004/146‑1 (MQ=255)
           cGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGTCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGcc                                                                                                                                        <  2:43386/146‑1 (MQ=255)
           cGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTg                                                                                                                                      >  2:19049/1‑148 (MQ=255)
           cGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCggtca                                                                                                                                       <  8:280502/147‑4 (MQ=255)
             gcgcAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCAGaaa                                                                                                                                   >  2:193366/1‑149 (MQ=255)
               gcAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCAGAAAcc                                                                                                                                 <  1:193366/149‑1 (MQ=255)
                   gCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTc                                                                                                                             >  8:97188/1‑149 (MQ=255)
                   gCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCCGAAACCGTTc                                                                                                                             >  8:4222/1‑149 (MQ=255)
                   gCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCAGAAAccatgc                                                                                                                             <  1:255649/149‑5 (MQ=255)
                        gTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTcgacga                                                                                                                        <  1:334594/149‑1 (MQ=255)
                           aaGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTcga                                                                                                                           <  6:348679/143‑1 (MQ=255)
                           aaGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTcga                                                                                                                           >  5:348679/1‑143 (MQ=255)
                            aGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTg                                                                                                                    >  8:293152/1‑149 (MQ=255)
                                 aGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGcgccc                                                                                                               >  4:125360/1‑149 (MQ=255)
                                         cccGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGa                                                                                                       <  2:205088/149‑1 (MQ=255)
                                           cGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCCGCCCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGCGCTGCGCccgccg                                                                                                           >  7:324750/1‑143 (MQ=255)
                                            gAAAGAAGCCCATGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGAtct                                                                                                    <  5:248335/149‑1 (MQ=255)
                                             aaaGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGAtctc                                                                                                   <  6:119453/149‑1 (MQ=255)
                                             aaaGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGAtctc                                                                                                   <  5:219086/149‑1 (MQ=255)
                                             aaaGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGAtctc                                                                                                   <  7:293152/149‑1 (MQ=255)
                                             aaaGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCCGAAACCGTTCGACGAGCTGCGCCCGCCGAAGAtctc                                                                                                   <  7:4222/149‑1 (MQ=255)
                                                            cTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTggg                                                                                    >  4:26370/1‑149 (MQ=255)
                                                                  aaCTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGc                                                                              >  4:183021/1‑149 (MQ=255)
                                                                       gATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTgg                                                                                     <  1:58447/137‑1 (MQ=255)
                                                                       gATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTgg                                                                                     >  2:58447/1‑137 (MQ=255)
                                                                       gATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAg                                                                          <  3:125360/148‑1 (MQ=255)
                                                                           gCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGc                                                                     <  3:183021/149‑1 (MQ=255)
                                                                                ctaagaagGCCAGCGCGTTATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCTCCCTCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCg                                                                <  3:211398/147‑1 (MQ=255)
                                                                                 gaagaaGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCg                                                                >  6:91221/1‑148 (MQ=255)
                                                                                    gacggCCAGCGCGTGCTCACCCCGACCACCGCCGAGTACCCCCTGCACAAGGGCCTGGGTTCTGACCGCGGCCTGAAACCGTGCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGCCCTGGGCAATGCCGAGGAAGCCATCGAACt                                                            <  8:321897/146‑1 (MQ=255)
                                                                                              gcgTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAg                                                  >  6:26298/1‑149 (MQ=255)
                                                                                                              caccGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCtgat                                  <  5:26298/149‑1 (MQ=255)
                                                                                                              caccGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCtgat                                  >  8:227240/1‑149 (MQ=255)
                                                                                                                ccgccgAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCtgatga                                <  1:19049/149‑1 (MQ=255)
                                                                                                                       gTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTgccgc                         <  5:91221/149‑1 (MQ=255)
                                                                                                                            cACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGt                    >  2:50428/1‑149 (MQ=255)
                                                                                                                             aCTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTc                   >  4:82374/1‑149 (MQ=255)
                                                                                                                                  acaAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCCGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAAc              >  3:95069/1‑149 (MQ=255)
                                                                                                                                         cATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGcc       >  3:250454/1‑149 (MQ=255)
                                                                                                                                           cggTTTCAGGCCGCGGTCAGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCgg     >  3:144233/2‑149 (MQ=255)
                                                                                                                                            ggTTTCTGACCGCGGCCCGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCgg     >  6:168999/1‑148 (MQ=255)
                                                                                                                                              tttCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTAc  >  4:90825/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GTCACTATCTCCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTAC  >  NC_002947/5550515‑5550805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑