Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,550,663 A→G 10.4% D302G (GAC→GGC)  PP_4881 → iron ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,550,6630AG10.4% 120.2 / 5.5 48D302G (GAC→GGC) PP_4881iron ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (22/21);  new base G (2/3);  total (24/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.41e-01

TCACTATCTCCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTC  >  NC_002947/5550516‑5550810
                                                                                                                                                   |                                                                                                                                                   
tCACTATCTCCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGAc                                                                                                                                                    <  1:93841/149‑1 (MQ=255)
  aCTATCTCCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGAcc                                                                                                                                                   <  8:121639/148‑1 (MQ=255)
  aCTATCTCCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACcg                                                                                                                                                  >  5:119453/1‑149 (MQ=255)
        tcCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCt                                                                                                                                            >  1:205088/1‑149 (MQ=255)
         ccGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGc                                                                                                                                              <  6:79340/146‑1 (MQ=255)
         ccGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGc                                                                                                                                              >  5:79340/1‑146 (MQ=255)
          cGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCATCTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGAGCACCGCCGAGGAGCCACTGCACAAGGGCTTGGTTTCTGACCGCGGcc                                                                                                                                             <  5:155004/146‑1 (MQ=255)
          cGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGTCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGcc                                                                                                                                             <  2:43386/146‑1 (MQ=255)
          cGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTg                                                                                                                                           >  2:19049/1‑148 (MQ=255)
          cGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCggtca                                                                                                                                            <  8:280502/147‑4 (MQ=255)
            gcgcAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCAGaaa                                                                                                                                        >  2:193366/1‑149 (MQ=255)
              gcAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCAGAAAcc                                                                                                                                      <  1:193366/149‑1 (MQ=255)
                  gCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTc                                                                                                                                  >  8:97188/1‑149 (MQ=255)
                  gCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCCGAAACCGTTc                                                                                                                                  >  8:4222/1‑149 (MQ=255)
                  gCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCAGGCCGCGGTCAGAAAccatgc                                                                                                                                  <  1:255649/149‑5 (MQ=255)
                       gTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTcgacga                                                                                                                             <  1:334594/149‑1 (MQ=255)
                          aaGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTcga                                                                                                                                <  6:348679/143‑1 (MQ=255)
                          aaGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTcga                                                                                                                                >  5:348679/1‑143 (MQ=255)
                           aGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTg                                                                                                                         >  8:293152/1‑149 (MQ=255)
                                aGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGcgccc                                                                                                                    >  4:125360/1‑149 (MQ=255)
                                        cccGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGa                                                                                                            <  2:205088/149‑1 (MQ=255)
                                          cGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCCGCCCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGCGCTGCGCccgccg                                                                                                                >  7:324750/1‑143 (MQ=255)
                                           gAAAGAAGCCCATGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGAtct                                                                                                         <  5:248335/149‑1 (MQ=255)
                                            aaaGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGAtctc                                                                                                        <  7:293152/149‑1 (MQ=255)
                                            aaaGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGAtctc                                                                                                        <  6:119453/149‑1 (MQ=255)
                                            aaaGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGAtctc                                                                                                        <  5:219086/149‑1 (MQ=255)
                                            aaaGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCCGAAACCGTTCGACGAGCTGCGCCCGCCGAAGAtctc                                                                                                        <  7:4222/149‑1 (MQ=255)
                                                           cTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTggg                                                                                         >  4:26370/1‑149 (MQ=255)
                                                                 aaCTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGc                                                                                   >  4:183021/1‑149 (MQ=255)
                                                                      gATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTgg                                                                                          >  2:58447/1‑137 (MQ=255)
                                                                      gATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTgg                                                                                          <  1:58447/137‑1 (MQ=255)
                                                                      gATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAg                                                                               <  3:125360/148‑1 (MQ=255)
                                                                          gCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGc                                                                          <  3:183021/149‑1 (MQ=255)
                                                                               ctaagaagGCCAGCGCGTTATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCTCCCTCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCg                                                                     <  3:211398/147‑1 (MQ=255)
                                                                                gaagaaGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCg                                                                     >  6:91221/1‑148 (MQ=255)
                                                                                   gacggCCAGCGCGTGCTCACCCCGACCACCGCCGAGTACCCCCTGCACAAGGGCCTGGGTTCTGACCGCGGCCTGAAACCGTGCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGCCCTGGGCAATGCCGAGGAAGCCATCGAACt                                                                 <  8:321897/146‑1 (MQ=255)
                                                                                             gcgTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAg                                                       >  6:26298/1‑149 (MQ=255)
                                                                                                             caccGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCtgat                                       >  8:227240/1‑149 (MQ=255)
                                                                                                             caccGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCtgat                                       <  5:26298/149‑1 (MQ=255)
                                                                                                               ccgccgAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCtgatga                                     <  1:19049/149‑1 (MQ=255)
                                                                                                                      gTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTgccgc                              <  5:91221/149‑1 (MQ=255)
                                                                                                                           cACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGt                         >  2:50428/1‑149 (MQ=255)
                                                                                                                            aCTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTc                        >  4:82374/1‑149 (MQ=255)
                                                                                                                                 acaAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCCGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAAc                   >  3:95069/1‑149 (MQ=255)
                                                                                                                                        cATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGcc            >  3:250454/1‑149 (MQ=255)
                                                                                                                                          cggTTTCAGGCCGCGGTCAGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCgg          >  3:144233/2‑149 (MQ=255)
                                                                                                                                           ggTTTCTGACCGCGGCCCGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCgg          >  6:168999/1‑148 (MQ=255)
                                                                                                                                             tttCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTAc       >  4:90825/1‑149 (MQ=255)
                                                                                                                                                   aCCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTc  <  3:90825/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCACTATCTCCGGCGCAGGCGTGGTCAAGGCCAGCAAACACCCGAAAGAAGCCCAGGCCCTGCTCAACTGGATGGCCAGCGAAGAAGGCCAGCGCGTGATCACCCAGACCACCGCCGAGTACCCACTGCACAAGGGCATGGTTTCTGACCGCGGCCTGAAACCGTTCGACGAGCTGCGCCCGCCGAAGATCTCGCCAGCTGACCTGGGCAATGCCGAGGAAGCCATCGAACTTGAGCGTGAGGTCGGCCTGCTCTGATGACTGCCGCCCTGTCCGAACCGGTGCCGGTACGCTTC  >  NC_002947/5550516‑5550810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑