Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,554,882 T→C 7.3% E5G (GAA→GGA) ‡ PP_4886 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,554,8820TC 7.3% 239.2 / 5.8 82E5G (GAA→GGA) ‡PP_4886hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (42/34);  new base C (3/3);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTGGGAAATCTCCTACAGATTC  >  NC_002947/5554734‑5555030
                                                                                                                                                    |                                                                                                                                                    
gTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGtt                                                                                                                                                      <  5:50608/149‑1 (MQ=255)
    gCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGa                                                                                                                                                  <  1:201348/149‑1 (MQ=255)
      aCCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGtt                                                                                                                                                      <  2:258405/143‑1 (MQ=255)
      aCCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGGGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTAGGCTGATAACGACGATGTTCAGa                                                                                                                                                  >  1:258405/1‑147 (MQ=255)
           aCTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTCTGAACATCGTc                                                                                                                                           <  6:136441/149‑1 (MQ=255)
           aCTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTCTGAACATCGTc                                                                                                                                           >  5:136441/1‑149 (MQ=255)
             ttGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTc                                                                                                                                           <  4:285376/147‑1 (MQ=255)
             ttGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTc                                                                                                                                           >  3:285376/1‑147 (MQ=255)
                tAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATcc                                                                                                                                       <  4:252179/148‑1 (MQ=255)
                       tgatTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGtt                                                                                                                                >  7:303505/1‑148 (MQ=255)
                          tttCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTc                                                                                                                            <  2:20285/149‑1 (MQ=255)
                           ttCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTcc                                                                                                                           >  3:92204/1‑149 (MQ=255)
                                tCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAg                                                                                                                      >  6:111016/1‑149 (MQ=255)
                                   aGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTTCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATc                                                                                                                                              >  8:110006/1‑122 (MQ=255)
                                   aGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTTCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATc                                                                                                                                              <  7:110006/122‑1 (MQ=255)
                                   aGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAg                                                                                                                      <  8:119858/146‑1 (MQ=255)
                                   aGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAg                                                                                                                      >  7:119858/1‑146 (MQ=255)
                                      tGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAAc                                                                                                                >  6:246727/1‑149 (MQ=255)
                                              tGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCg                                                                                                        >  6:293790/1‑149 (MQ=255)
                                              tGCTTATACAGTGCTTCGCCAGTGATTTCGGCGATATGATCATAAGCATCGAAGGCCATGCTTACCGCGCCCTGGGAAAGGTTCGGCAGATACAGACGATGTTCAGACATCGTCATCCTGAGGTTGATCCCGAAGTAAACAgccggccg                                                                                                        >  8:298714/1‑149 (MQ=255)
                                                cTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCgg                                                                                                       >  2:88714/1‑148 (MQ=255)
                                                 ttATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTa                                                                                                     >  3:154530/1‑149 (MQ=255)
                                                 ttATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTa                                                                                                     >  4:3815/1‑149 (MQ=255)
                                                  tATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTCTGAACATCGTcg                                                                                                                                          >  5:96090/1‑110 (MQ=255)
                                                  tATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTCTGAACATCGTcg                                                                                                                                          <  6:96090/111‑2 (MQ=255)
                                                   aTCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTa                                                                                                     <  4:92204/147‑1 (MQ=255)
                                                    tCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTacc                                                                                                   >  6:123989/1‑148 (MQ=255)
                                                     ccAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGtt                                                                                                                                                      <  4:50777/96‑1 (MQ=255)
                                                     ccAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTaccga                                                                                                 >  7:196108/1‑149 (MQ=255)
                                                          gCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTCTGAACATCGTc                                                                                                                                           >  8:161146/1‑102 (MQ=255)
                                                          gCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTCTGAACATCGTc                                                                                                                                           <  7:161146/102‑1 (MQ=255)
                                                              cGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTaccga                                                                                                 <  6:119031/140‑1 (MQ=255)
                                                              cGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTaccga                                                                                                 >  5:119031/1‑140 (MQ=255)
                                                              cGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCtt                                                                                          >  2:170886/1‑147 (MQ=255)
                                                               gCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAg                                                                                       <  8:303505/149‑1 (MQ=255)
                                                                ccAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAgg                                                                                      >  1:146790/1‑149 (MQ=255)
                                                                       tttCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATa                                                                               >  5:255577/1‑149 (MQ=255)
                                                                           cgcgATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATacaa                                                                            >  6:206520/1‑148 (MQ=255)
                                                                            gcgATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGTCGGTACCGgccgccgttt                                                                                        <  6:229048/135‑5 (MQ=255)
                                                                            gcgATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGTCGGTACCGgccgccgttt                                                                                        >  5:229048/1‑131 (MQ=255)
                                                                             cgATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAacac                                                                         <  5:206520/149‑1 (MQ=255)
                                                                                tatGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTaa                                                                      <  6:255577/149‑1 (MQ=255)
                                                                                  tGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAAcc                                                                    <  8:196108/149‑1 (MQ=255)
                                                                                    aaCATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGTCGGTACCGgccgccgtt                                                                                         <  4:229185/126‑4 (MQ=255)
                                                                                    aaCATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGTCGGTACCGgccgccgtt                                                                                         >  3:229185/1‑123 (MQ=255)
                                                                                     aCATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCt                                                                 <  5:246727/149‑1 (MQ=255)
                                                                                       ttAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTAt                                                               <  3:269315/148‑1 (MQ=255)
                                                                                         aaGCATCGAAAGCCATTCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATTTTCAGACATCGTAATCCTGAGGTTGCTACCGAAGTAAAAAGGAGGCCGGTACCGACCGCCTTCAGGCTCAATACAACAATAAACCCTATga                                                             >  4:269315/1‑148 (MQ=255)
                                                                                         aaGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGCCATCGTCATCCAGAGGTTGCTCCCGAAGTAAAGCGGCGGCCGGACCCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATg                                                              >  4:278926/1‑148 (MQ=255)
                                                                                         aaGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCa                                                                                        >  6:40752/1‑122 (MQ=255)
                                                                                         aaGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCa                                                                                        <  5:40752/122‑1 (MQ=255)
                                                                                         aaGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATg                                                              >  4:195296/1‑148 (MQ=255)
                                                                                         aaGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGt                                                             >  3:7797/1‑149 (MQ=255)
                                                                                           gCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGt                                                             >  5:67228/1‑147 (MQ=255)
                                                                                             aTCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGc                                                         <  5:293790/149‑1 (MQ=255)
                                                                                              tCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGc                                                         <  5:185349/148‑1 (MQ=255)
                                                                                              tCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGc                                                         >  6:185349/1‑148 (MQ=255)
                                                                                                  aaGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTg                                                    <  1:88714/149‑1 (MQ=255)
                                                                                                     ccATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCaa                                                 <  3:3815/149‑1 (MQ=255)
                                                                                                      cATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAAc                                                <  3:195296/149‑1 (MQ=255)
                                                                                                       aTGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAAGCTCAATACAACACAAAACCATATGTCAGACCTTGAAAc                                                >  2:335236/1‑148 (MQ=255)
                                                                                                             aCCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGc                                                                                     <  1:117304/105‑1 (MQ=255)
                                                                                                             aCCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGc                                                                                     >  2:117304/1‑105 (MQ=255)
                                                                                                             aCCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACa                                               >  8:262652/1‑143 (MQ=255)
                                                                                                             aCCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACa                                               <  7:262652/143‑1 (MQ=255)
                                                                                                               ctctCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGc                                       >  2:129003/1‑149 (MQ=255)
                                                                                                                   cccTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTa                                   >  1:24387/1‑149 (MQ=255)
                                                                                                                    ccTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTa                                   >  5:12997/1‑148 (MQ=255)
                                                                                                                    ccTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTAt                                  <  2:146790/149‑1 (MQ=255)
                                                                                                                        gTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAg                              <  6:67228/149‑1 (MQ=255)
                                                                                                                                  ggCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTgg                    <  8:262868/149‑1 (MQ=255)
                                                                                                                                    cTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTggg                   >  5:277292/1‑148 (MQ=255)
                                                                                                                                     tGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAg                              <  2:114630/136‑1 (MQ=255)
                                                                                                                                     tGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTGGGa                  >  3:190532/1‑148 (MQ=255)
                                                                                                                                     tGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATATCAGCCCTTGCAACAGCAAACGCCCTATCAAg                              >  1:114630/1‑136 (MQ=255)
                                                                                                                                      gATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTGGGaaa                >  4:123181/1‑149 (MQ=255)
                                                                                                                                          acgacgATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGc                                             <  3:277624/116‑1 (MQ=255)
                                                                                                                                          acgacgATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGc                                             >  4:277624/1‑116 (MQ=255)
                                                                                                                                           cgacgaTGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAATGAATATTTGGGAAATCTcc           <  4:7797/149‑1 (MQ=255)
                                                                                                                                            gacgaTGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTGGGAAATCTcc           >  5:172802/1‑148 (MQ=255)
                                                                                                                                             acgaTGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTGGGAAATCTCCTa         <  6:12997/149‑1 (MQ=255)
                                                                                                                                             acgaTGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTGGGAAATCTCCTa         <  2:24387/149‑1 (MQ=255)
                                                                                                                                              cgaTGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTGGGAAATCTCCTAc        <  1:170886/149‑1 (MQ=255)
                                                                                                                                               gaTGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTGGGAAATCTCCTa         >  7:289011/1‑147 (MQ=255)
                                                                                                                                               gaTGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTGGGAAATCTCCTACa       <  5:123989/149‑1 (MQ=255)
                                                                                                                                                    tCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGTCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTGGGAAATCTCCTACAGATTc  >  5:155464/1‑149 (MQ=255)
                                                                                                                                                    tCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTGGGAAATCTCCTACAGATTc  >  3:99022/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GTTGGCACCGAACTTGTAGCCGATGATTTCGATCCAGTTGGCGTACTGCTTATCCAGTGCTTCGCCAGTGATTTCCGCGATATGAACATAAGCATCGAAAGCCATGCTTACCTCTCCCTGGTAAAGGTTCGGCTGATAACGACGATGTTCAGACATCGTCATCCTGAGGTTGCTCCCGAAGTAAAACGGCGGCCGGTACCGACCGCCTTCAGGCTCAATACAACACTAACCCCTATGTCAGCCCTTGCAACAGCAAACGCCCTATCAAGGAATATTTGGGAAATCTCCTACAGATTC  >  NC_002947/5554734‑5555030

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑