Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,557,925 C→A 10.3% intergenic (‑296/+35) PP_4888 ← / ← purA methyl‑accepting chemotaxis transducer/adenylosuccinate synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,557,9250CA10.3% 141.9 / 7.6 56intergenic (‑296/+35)PP_4888/purAmethyl‑accepting chemotaxis transducer/adenylosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base C (24/26);  new base A (4/2);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01

CTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCTTCGATGCGCTTGATGTAGTCACGC  >  NC_002947/5557780‑5558068
                                                                                                                                                 |                                                                                                                                               
cTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAgg                                                                                                                                              >  1:5278/1‑149 (MQ=255)
    aaCGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCcg                                                                                                                                          >  7:232302/1‑149 (MQ=255)
      cGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCcgcg                                                                                                                                        >  7:188235/1‑149 (MQ=255)
       ggCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTGAGAAGGGCCgcgg                                                                                                                                       <  5:254747/149‑1 (MQ=255)
             cgcCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTc                                                                                                                                   <  8:188235/147‑1 (MQ=255)
                ccAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGAt                                                                                                                               >  7:294369/1‑148 (MQ=255)
                  aaCAATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCCGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTAAGGGCCGCGGCGTCAGaaaag                                                                                                                            >  6:226221/1‑145 (MQ=255)
                   aCCATCTTTGTGTGCCCACATAGGAGCGGTTTAACCCGCTAATACGGTAGGCGCGGCAACGGTGGATTACCGGTGTATATTGGCCAGCACCGACATGCCCACAAATTAAAACGCCGCGGCCCTTCTCTGGGCCGCGGCGTCAGATCAGc                                                                                                                           <  8:232302/149‑1 (MQ=255)
                       tCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGA‑TACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTgg                                                                                                                       >  6:278769/1‑148 (MQ=255)
                            gtgtgCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGA‑TACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGc                                                                                                                  <  5:278769/148‑1 (MQ=255)
                                gCCCACATACGAGCGGGTTTACCCGAGAATACGGTAGCGGCGGCAACGGTGCATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCACCGGCCCAACACAGGGCCGCCGCGTAAGATCAGCCTGGAATGCAGa                                                                                                               >  8:14219/1‑148 (MQ=25)
                                           aGCGGTTTTACCCGCGAATACGGTAGCGGGGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACgg                                                                                                   <  8:306301/149‑1 (MQ=255)
                                           aGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACgg                                                                                                   <  7:58225/149‑1 (MQ=255)
                                            gCGGGTTTACCCGCGAATACGGTAGCGGAGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACggg                                                                                                  >  3:267897/1‑149 (MQ=255)
                                             cGGGTTTACCCGCTAATACGGTAGCGGCGGAAACGGTGTATTACCGGTGGATATTGGCCAGCACAGAAATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACggg                                                                                                  <  7:14219/148‑1 (MQ=255)
                                                gTTTCCCCGGTAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCCGCACAGACATGCCCAAAATTCAAAACCCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGAc                                                                                              <  5:226221/149‑1 (MQ=255)
                                                     cccGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACgcagc                                                                                         >  5:107332/1‑149 (MQ=255)
                                                      ccGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACgcagca                                                                                        >  5:288076/1‑149 (MQ=255)
                                                      ccGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACgcagca                                                                                        >  5:257282/1‑149 (MQ=255)
                                                      ccGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACgcagca                                                                                        >  7:301306/1‑149 (MQ=255)
                                                           aaTACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAgg                                                                                                                                              >  6:202962/1‑90 (MQ=255)
                                                           aaTACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAgg                                                                                                                                              <  5:202962/90‑1 (MQ=255)
                                                            aTACGGTAGCGGCGGCAACGTGGGTTTTCCGGTGGATATTGGCCAGCACCGACTTGCCCACAAATCAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATg                                                                                   <  5:222834/148‑1 (MQ=255)
                                                            aTACGGAAGCGGCGGCAACGGTGTATTACGGGTGTATATTGGCCAGCACATACATGCCCACAACTGAAAACCCCGGGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATg                                                                                   <  7:204532/148‑1 (MQ=255)
                                                                ggTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGtctc                                                                              <  6:269493/149‑1 (MQ=255)
                                                                    gcggcgGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGtt                                                                           <  8:171498/148‑1 (MQ=255)
                                                                            aaCGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCgg                                                                  <  3:306321/149‑1 (MQ=255)
                                                                               ggTGGATTACCGGTGTATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGACGCGGCGTCAGATCAGACTGTAAGGAAGATCAGGCGCACGGGTGACGCAGACCGATGGTCTCGTTGCCGTCCGGGcg                                                               >  2:283551/1‑148 (MQ=255)
                                                                                     ttACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCg                                                          >  1:7163/1‑148 (MQ=255)
                                                                                         cGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAatg                                                     <  8:301306/149‑1 (MQ=255)
                                                                                           gTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAatgat                                                   >  6:195795/1‑149 (MQ=255)
                                                                                           gTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCCGCCCATCTCAGCGCCCCCGCGTCAGATCAGCCAGGAAGGCAGATCACGCGAACCCGTGACCCAGCACGATCGTCTCCTTGCGGTCCGGCCGGCTCGAAatgaa                                                   >  6:190943/1‑148 (MQ=255)
                                                                                              gATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCTGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGt                                                 <  8:294369/148‑1 (MQ=255)
                                                                                                tatTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGa                                              >  2:61365/1‑149 (MQ=255)
                                                                                                tatTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGa                                              >  1:211087/1‑149 (MQ=255)
                                                                                                 atTTGCCAGCAGAGAGATTCCCAGAATTGATAAGGCCGGGGCGCTTGTCAGGGCCGCGGCGTCAGATCAGGCTGGAAGGGAGATCAGGCGTACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTGGa                                              <  6:107332/148‑1 (MQ=255)
                                                                                                  ttGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGAt                                             <  6:288076/148‑1 (MQ=255)
                                                                                                              gACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAAcc                                <  3:144899/149‑1 (MQ=255)
                                                                                                              gACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAAcc                                <  2:5278/149‑1 (MQ=255)
                                                                                                                  tGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGct                            <  5:195795/149‑1 (MQ=255)
                                                                                                                   gCCCACTAATGAAAACGCGGCGCCCGTTCTCAGGGCCGGGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGct                            <  5:190943/148‑1 (MQ=255)
                                                                                                                    cccACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGctct                          >  6:344673/1‑149 (MQ=255)
                                                                                                                     ccACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCtt                         <  6:257282/149‑1 (MQ=255)
                                                                                                                      cgatattGAAACGGCCGCGGCGCTTCTCTGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCtt                         <  4:4665/142‑1 (MQ=255)
                                                                                                                             gAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCtt                         >  3:4665/1‑141 (MQ=255)
                                                                                                                              aaaaCGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACTAGCTCTTCGATgcgc                 <  2:211087/148‑1 (MQ=255)
                                                                                                                              aaaaCGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCTTCGATGCGCt                >  2:14037/1‑149 (MQ=255)
                                                                                                                               tgaCGCCGCGGCCCTGAGAAGGGCCGCGGCGTCAGATAAGCCAGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTATCGTTTCGGTCCGGGCCCGTCGAAATGATTTCGATCGGCCCGCCAACCAGCACATCGATTCGCtt               >  4:283935/3‑149 (MQ=255)
                                                                                                                                 aCGCCGCGGCCCTGTGAAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCTTCGATGCGCtt               <  3:283935/147‑1 (MQ=255)
                                                                                                                                   gccgcGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCg                                                              >  5:289086/1‑98 (MQ=255)
                                                                                                                                   gccgcGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCg                                                              <  6:289086/98‑1 (MQ=255)
                                                                                                                                   gccgcGGCCCTGAGAAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCTTCGATGCGCTTGATg           >  6:10696/1‑149 (MQ=255)
                                                                                                                                   gccgcGGCCCTGAGAAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCTTCGATGCGCTTGATg           >  6:15601/1‑149 (MQ=255)
                                                                                                                                           ccTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATg                                                                                   >  3:101148/1‑69 (MQ=255)
                                                                                                                                           ccTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATg                                                                                   <  4:101148/69‑1 (MQ=255)
                                                                                                                                            cTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCTTCGATGCGCTTGATGTAGTCAcgc  >  3:8988/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTACAACGGCCTGCGCCCAACCATCGTTGTGTGCCCACATAGGAGCGGGTTTACCCGCGAATACGGTAGCGGCGGCAACGGTGGATTACCGGTGGATATTGGCCAGCACAGACATGCCCACAAATGAAAACGCCGCGGCCCTTCTCAGGGCCGCGGCGTCAGATCAGCCTGGAAGGCAGATCAGGCGAACGGGTGACGCAGCACGATGGTCTCGTTGCGGTCCGGGCCGGTCGAAATGATGTCGATCGGCGCGCCAACCAGCTCTTCGATGCGCTTGATGTAGTCACGC  >  NC_002947/5557780‑5558068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑