Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,582,813 T→A 7.9% H178L (CAC→CTC)  PP_4911 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,582,8130TA 7.9% 197.3 / 4.0 70H178L (CAC→CTC) PP_4911hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (30/33);  new base A (4/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGC  >  NC_002947/5582679‑5582960
                                                                                                                                      |                                                                                                                                                   
cAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCGGGTTGGCCAGCGGCTTCCCCGCCGTGCTCCGCGGTTCGGCCAGCGTCTGCCACAGTTGGCCCTGCGCCTGCCGCTGCGCCGACTCACGGCCCTCGGGCTGGTGGTCGCgg                                                                                                                                       >  8:117935/1‑149 (MQ=255)
   cGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTgg                                                                                                                                    <  3:149433/149‑1 (MQ=255)
   cGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTgg                                                                                                                                    <  6:298043/149‑1 (MQ=255)
       cTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGt                                                                                                                                      >  4:283355/1‑143 (MQ=255)
       cTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGt                                                                                                                                      <  3:283355/143‑1 (MQ=255)
        tttttCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGgatga                                                                                                                                >  6:138249/1‑148 (MQ=255)
        tttttCGCTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGAAACAGTTCGACCTGAGCCTGCACCTGCGACTACTCACTGCCCTCGTGCTGCTCCACGAGGTGgatga                                                                                                                                >  4:173884/1‑148 (MQ=255)
                  ccTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCa                                                                                                                     >  8:160975/1‑149 (MQ=255)
                  ccTCTTCGGCAATCGCCGGCTGGTTGACCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCa                                                                                                                     >  1:9969/1‑149 (MQ=255)
                        cGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTACACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGc                                                                                                                  <  6:52949/146‑1 (MQ=255)
                          gCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTGTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATg                                                                                                             <  3:278296/149‑1 (MQ=255)
                          gCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATg                                                                                                             <  2:11650/149‑1 (MQ=255)
                          gCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATg                                                                                                             >  2:88004/1‑149 (MQ=255)
                           cAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGCGTAACTCCCCTCGTGCGGCTGCTCGAGGTGGTTGATACGGTGTTCCCGCACATgg                                                                                                            >  3:48943/1‑149 (MQ=255)
                                gCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCAcg                                                                                                       >  6:36949/1‑149 (MQ=255)
                                     cTGGTTGGCCAGCGGCTTCACCTCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGt                                                                                                  <  6:290331/149‑1 (MQ=255)
                                        gTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCaa                                                                                               <  4:122657/149‑1 (MQ=255)
                                        gTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCCGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTc                                                                                                 >  1:57410/1‑147 (MQ=255)
                                         ttGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAg                                                                                              <  4:93032/149‑1 (MQ=255)
                                           ggCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGc                                                                                             <  5:36949/148‑1 (MQ=255)
                                             ccAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGa                                                                                          >  7:18220/1‑149 (MQ=255)
                                              cAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGAc                                                                                         <  7:117935/149‑1 (MQ=255)
                                                   gCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCAc                                                                                    <  5:138249/149‑1 (MQ=255)
                                                    cTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGgat                                                                                                                                  <  7:162755/102‑1 (MQ=255)
                                                    cTTCACCGCCGTGCTCAGCTGTTCCGACAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCCATGTCgat                                                                                                                                  >  8:162755/1‑102 (MQ=255)
                                                     ttCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTg                                                                                  >  6:163798/1‑149 (MQ=255)
                                                       cACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGGCTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTggg                                                                                >  4:312803/1‑149 (MQ=255)
                                                       cACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTgg                                                                                 <  2:9969/148‑1 (MQ=255)
                                                         ccgccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACCGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCa                                                                              >  1:214545/1‑149 (MQ=255)
                                                          cgccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCAt                                                                             >  7:55858/1‑149 (MQ=255)
                                                          cgccgTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCAt                                                                             <  8:55858/149‑1 (MQ=255)
                                                                  tCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATg                                                                     >  3:26992/1‑149 (MQ=255)
                                                                       tGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCgg                                                                >  7:255562/1‑149 (MQ=255)
                                                                            ggCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTgg                                                            <  7:160975/148‑1 (MQ=255)
                                                                            ggCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTgg                                                            <  7:142732/148‑1 (MQ=255)
                                                                              ccAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTg                                                                                  >  4:223465/1‑124 (MQ=255)
                                                                              ccAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTg                                                                                  <  3:223465/124‑1 (MQ=255)
                                                                               cAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGt                                                                                                                                                     >  3:316204/1‑56 (MQ=255)
                                                                               cAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGt                                                                                                                                                     <  4:316204/56‑1 (MQ=255)
                                                                                  cTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCTCTGGGCATCCAGGATGGCCGGCTGGTTCTTGc                                                     <  8:18220/149‑1 (MQ=255)
                                                                                        cAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAAt                                               >  4:86034/1‑149 (MQ=255)
                                                                                        cAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAAt                                               >  6:137396/1‑149 (MQ=255)
                                                                                           cAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCa                                            <  8:316707/149‑1 (MQ=255)
                                                                                              ttGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGc                                         <  5:163798/149‑1 (MQ=255)
                                                                                                ggCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCgt                                       >  6:327515/1‑149 (MQ=255)
                                                                                                 gCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCgtg                                      >  5:218253/1‑149 (MQ=255)
                                                                                                  cccTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCgtgt                                     <  1:88004/149‑1 (MQ=255)
                                                                                                   ccTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGt                                                           <  6:316680/126‑1 (MQ=255)
                                                                                                   ccTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGt                                                           >  5:316680/1‑126 (MQ=255)
                                                                                                       aGCCTGCACCTGCGACGGCTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATc                                 <  3:312803/148‑1 (MQ=255)
                                                                                                       aGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGc                                         <  2:189199/140‑1 (MQ=255)
                                                                                                       aGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGc                                         >  1:189199/1‑140 (MQ=255)
                                                                                                        gcgtGCACCTGCGAAGACTCACTGCCCTGGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGa                               <  3:173884/146‑1 (MQ=255)
                                                                                                        gCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGa                               <  4:26992/149‑1 (MQ=255)
                                                                                                         ccTGCACCTGCGTCGACTAACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGac                              <  6:201409/149‑1 (MQ=255)
                                                                                                          cTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGAATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCgtg                                      >  5:194674/1‑140 (MQ=255)
                                                                                                              aCCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAg                         >  5:1117/1‑149 (MQ=255)
                                                                                                                       gacTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCAc                <  6:218253/149‑1 (MQ=255)
                                                                                                                            aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTc             <  5:327515/147‑1 (MQ=255)
                                                                                                                            aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCg           >  8:167464/1‑149 (MQ=255)
                                                                                                                            aCTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCg           >  7:6778/1‑149 (MQ=255)
                                                                                                                             tccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGTTGGTTCTTGCCAGAATCCAGGCGtgtg                                    <  6:343523/119‑1 (MQ=255)
                                                                                                                             tccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGTTGGTTCTTGCCAGAATCCAGGCGtgtg                                    >  5:343523/5‑123 (MQ=255)
                                                                                                                              cgCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCggg         <  2:214545/148‑1 (MQ=255)
                                                                                                                              ccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGTGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCtt                                                       <  3:318269/100‑1 (MQ=255)
                                                                                                                              ccaccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGTGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCtt                                                       >  4:318269/4‑103 (MQ=255)
                                                                                                                               caccTCGAGCAGCAGCACGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGc        >  3:105436/3‑149 (MQ=255)
                                                                                                                                cccTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGc        <  6:1117/148‑1 (MQ=255)
                                                                                                                                  cTCGAGCTGCTGCTCGAGGTGGATGATACGCTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGCGCATCCAGGATGGACGTCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGacaa                            >  5:228976/1‑126 (MQ=255)
                                                                                                                                   tCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGTCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTg      >  5:41566/1‑147 (MQ=255)
                                                                                                                                   tCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTg      >  6:107594/1‑147 (MQ=255)
                                                                                                                                     gTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGc  <  5:137396/149‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                                   
CAGCGGGCTTTTTCGGTGCCTCTTCGGCAATCGCCGGCTGGTTGGCCAGCGGCTTCACCGCCGTGCTCAGCTGTTCGGCCAGCTTCTGCAACAGTTGGCCCTGAGCCTGCACCTGCGACGACTCACTGCCCTCGTGCTGCTGCTCGAGGTGGATGATACGGTTGTCACGCACATGGCCACGGCGGTCAAGCAGACGCCACTGGGCATCCAGGATGGCCGGCTGGTTCTTGCCAGAATCCAGGCGTGTGATCGACAACAGCACTTGCACGTCCGGGCTGAAGC  >  NC_002947/5582679‑5582960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑