Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,587,808 T→C 7.9% K447R (AAG→AGG)  parE ← DNA topoisomerase IV subunit B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,587,8080TC 7.9% 179.6 / 5.0 63K447R (AAG→AGG) parEDNA topoisomerase IV subunit B
Reads supporting (aligned to +/- strand):  ref base T (32/26);  new base C (3/2);  total (35/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

AGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTGCACAATCGGCCAGCTTGCCGGGCAATG  >  NC_002947/5587663‑5587949
                                                                                                                                                 |                                                                                                                                             
aGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTg                                                                                                                                             <  7:95465/148‑1 (MQ=255)
aGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGc                                                                                                                                            >  8:225846/1‑149 (MQ=255)
 gCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGcc                                                                                                                                           <  1:260263/149‑1 (MQ=255)
 gCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGcc                                                                                                                                           >  7:104116/1‑149 (MQ=255)
     cGGCGAGGATGCAGATCTTGCCATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCCTGCcgctg                                                                                                                                       >  6:249976/1‑147 (MQ=255)
       gCGAGGATGCAGATCTTGCCATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAg                                                                                                                                     >  4:114923/1‑149 (MQ=255)
                 aGATCTTGCCATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATg                                                                                                                           >  1:322087/1‑149 (MQ=255)
                 aGATCTTGCCATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATg                                                                                                                           >  3:181809/1‑149 (MQ=255)
                 aGATCTTGCCATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCCTGCCGCTGCGCGGCAAGAtc                                                                                                                           <  5:249976/149‑2 (MQ=255)
                         ccATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGa                                                                                                                    <  8:207090/148‑1 (MQ=255)
                          cATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAAt                                                                                                                  >  1:73550/1‑149 (MQ=255)
                          cATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAAt                                                                                                                  >  6:282896/1‑149 (MQ=255)
                          cATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAAt                                                                                                                  >  3:91880/1‑149 (MQ=255)
                          cATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAAt                                                                                                                  >  3:6700/1‑149 (MQ=255)
                           aTAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAAtt                                                                                                                 >  4:305377/1‑149 (MQ=255)
                           aTAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAAtt                                                                                                                 <  6:153877/149‑1 (MQ=255)
                            tAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTc                                                                                                                <  3:305377/149‑1 (MQ=255)
                              gcgcAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTcc                                                                                                               <  3:298001/148‑1 (MQ=255)
                                gcAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTg                                                                                                            <  4:181809/149‑1 (MQ=255)
                                 cAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGt                                                                                                           >  7:180196/1‑149 (MQ=255)
                                              tCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCtt                                                                                              <  2:216873/149‑1 (MQ=255)
                                                ggTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTgg                                                                                            <  5:282896/149‑1 (MQ=255)
                                                  ttGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGcc                                                                                          >  4:18590/1‑149 (MQ=255)
                                                             tcgtcgCCTATGGCCACGGGAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGGATGATGGCCTGGAATTCCTTGTCCCGCTCCTGCTTGGCCGAACCACCgg                                                                                <  8:111473/148‑1 (MQ=255)
                                                                aCGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGa                                                                            <  3:114923/149‑1 (MQ=255)
                                                                aCGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCCTGCCGCTGCGCGGCAAGAtc                                                                                                                           <  3:121150/102‑2 (MQ=255)
                                                                aCGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCCTGCCGCTGCGCGGCAAGAtc                                                                                                                           >  4:121150/1‑101 (MQ=255)
                                                                   ccAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAac                                                                                      <  1:307155/136‑1 (MQ=255)
                                                                   ccAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAac                                                                                      >  2:307155/1‑136 (MQ=255)
                                                                           cACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCAcc                                                                      >  5:53419/1‑144 (MQ=255)
                                                                           cACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCAcc                                                                      <  6:53419/144‑1 (MQ=255)
                                                                           cACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTcga                                                                 <  3:18590/149‑1 (MQ=255)
                                                                              ggCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTcgac                                                                >  1:336341/1‑147 (MQ=255)
                                                                              ggCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTcgac                                                                <  2:336341/147‑1 (MQ=255)
                                                                               gCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAg                                                             >  1:57655/1‑149 (MQ=255)
                                                                                      tgtgCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTc                                                                                                          >  5:106768/1‑97 (MQ=255)
                                                                                      tgtgCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTc                                                                                                          <  6:106768/97‑1 (MQ=255)
                                                                                        tgCAGTTCCTGTGTGGCCAGGACTTCGCCCCCGTCCTCTTCACTGGTGTTGAGGATCTTGCCGCGCAGCTGCTGGATGGCCTGGATTTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCAGAGTGAGGCTCGACGAGGAACAAGTc                                                    <  4:91880/149‑1 (MQ=255)
                                                                                        tgCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTc                                                    >  4:326831/1‑149 (MQ=255)
                                                                                                     tggcCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCggg                                       >  2:212585/1‑149 (MQ=255)
                                                                                                      ggcCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGt                                      >  2:106502/1‑149 (MQ=255)
                                                                                                       gcCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCggg                                       <  7:225846/147‑1 (MQ=255)
                                                                                                         cAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACCACTCGGCACGCATCGGGTCCt                                   >  6:346692/1‑149 (MQ=255)
                                                                                                         cAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCAGCACGCATCGGGTCCt                                   >  8:249961/1‑149 (MQ=255)
                                                                                                         cAGGACTTCGCCCCCGCCCACTTCCCAGGTGTTGAGGATCTTGCCGCCCAGCGCCAGGATGGCCTCGAATTCCTTGTCCCGCGCCTCCTTGGCCGAACCACCGGCCGAGTCACCCTCGACCAGGAACAACACGGCACCCATCgcgaccg                                   >  3:23346/1‑143 (MQ=255)
                                                                                                          aGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGTGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAgg                                                            <  5:73381/123‑1 (MQ=255)
                                                                                                          aGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGTGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAgg                                                            >  6:73381/1‑123 (MQ=255)
                                                                                                          aGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTg                                  >  8:24221/1‑149 (MQ=255)
                                                                                                          aGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTg                                  >  6:59447/1‑149 (MQ=255)
                                                                                                          aGGACTTCGCCCCCGACCACTTCCGAGGGGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCAGCCTCGACGAGGAACAACTCGGCACGCATCGGGGCCTg                                  <  4:23346/149‑1 (MQ=255)
                                                                                                               ttCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTg                             >  3:291145/1‑149 (MQ=255)
                                                                                                               ttCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTg                             >  2:254463/1‑149 (MQ=255)
                                                                                                                    ccccGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTGCACaa                        >  5:241895/1‑149 (MQ=255)
                                                                                                                      ccGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTGCACAATc                      <  2:322087/149‑1 (MQ=255)
                                                                                                                        ggccACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTGCACAATCgg                    <  8:160090/147‑1 (MQ=255)
                                                                                                                           cACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTGCACAATCGGc                   >  2:121863/1‑147 (MQ=255)
                                                                                                                           cACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTGCACAATCGGCCa                 <  2:73550/149‑1 (MQ=255)
                                                                                                                                     gtgtTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTGCACAATCGGCCAGCTTGCCggg       <  1:212585/149‑1 (MQ=255)
                                                                                                                                          ggccaTCCTGCCGCTGCGCGGCAAGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTGCACAATCGGCCAGCTTGCCGGGCAATg  >  5:55671/5‑149 (MQ=255)
                                                                                                                                          gAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGTGCCTGCtt                                                                                              <  7:8914/57‑1 (MQ=255)
                                                                                                                                          gAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGTGCCTGCtt                                                                                              >  8:8914/1‑57 (MQ=255)
                                                                                                                                             gATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTGCACAATCGGCCAGCTTGc           >  6:110446/1‑137 (MQ=255)
                                                                                                                                             gATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTGCACAATCGGCCAGCTTGc           <  5:110446/137‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
AGCGTCGGCGAGGATGCAGATCTTGCCATAGCGCAGCTGCGCAAGGTCGGTTGCACCCGGATCGACGCCAATGGCCACGGCAATGTTGTGCACTTCCTGGCTGGCCAGGACTTCGCCCCCGTCCACTTCCCAGGTGTTGAGGATCTTGCCGCGCAGCGGCAGGATGGCCTGGAATTCCTTGTCCCGCGCCTGCTTGGCCGAACCACCGGCCGAGTCACCCTCGACGAGGAACAACTCGGCACGCATCGGGTCCTGGCCTGCACAATCGGCCAGCTTGCCGGGCAATG  >  NC_002947/5587663‑5587949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑