Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,608,300 G→C 8.5% intergenic (‑162/+26) cyaB ← / ← waaA cyclolysin secretion/processing ATP‑binding protein CyaB/3‑deoxy‑D‑manno‑octulosonic acid transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,608,3000GC 8.5% 136.5 / 3.3 47intergenic (‑162/+26)cyaB/waaAcyclolysin secretion/processing ATP‑binding protein CyaB/3‑deoxy‑D‑manno‑octulosonic acid transferase
Reads supporting (aligned to +/- strand):  ref base G (21/22);  new base C (2/2);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

AAGCTTCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGTTCCACCAGGCG  >  NC_002947/5608156‑5608445
                                                                                                                                                 |                                                                                                                                                 
aaGCTTCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTc                                                                                                                                               >  7:10794/1‑149 (MQ=255)
      ccGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGa                                                                                                                                          <  8:158299/148‑1 (MQ=255)
          cGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGa                                                                                                                                     <  2:155324/149‑1 (MQ=255)
           gCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTATAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGat                                                                                                                                    <  4:60614/149‑1 (MQ=255)
             aaGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTCAAAGACCCGGTC‑TAGAGTGGatat                                                                                                                                  <  5:195478/149‑1 (MQ=39)
             aaGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCgg                                                                                                                                                  <  1:265004/133‑1 (MQ=255)
             aaGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCgg                                                                                                                                                  >  2:265004/1‑133 (MQ=255)
                 gtgtGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCg                                                                                                                              <  8:97591/149‑1 (MQ=255)
                  tgtgACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCgg                                                                                                                             <  8:10794/149‑1 (MQ=255)
                      acacTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCTGTTAt                                                                                                                         >  8:295959/1‑149 (MQ=255)
                      acacTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTa                                                                                                                          >  8:300536/1‑148 (MQ=255)
                              gACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGa                                                                                                                                          <  3:235215/124‑1 (MQ=255)
                              gACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGa                                                                                                                                          >  4:235215/1‑124 (MQ=255)
                               aCTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGAt                                                                                                                 <  8:127128/148‑1 (MQ=255)
                                      aaGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGccc                                                                                                         >  4:13094/1‑149 (MQ=255)
                                                gTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTc                                                                                               <  3:13094/149‑1 (MQ=255)
                                                 tAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCACg                                                                                              >  8:66175/1‑149 (MQ=255)
                                                    gAAGTTTCCTAACGGAGAGAAATCCGGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCATAATCAGACATATGCTCTC‑TAGTGCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCGCAGACCCTCGAg                                                                                            <  6:349304/148‑1 (MQ=255)
                                                     aaGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGc                                                                                           >  5:111196/1‑148 (MQ=255)
                                                           ccTAACGGCGAGTAATCCATTGTATGAAGTTACAACTTACGCCGTAGGATTAAATAACCAGAATCAGACATATCCACTC‑TAGACCGTGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGc                                                                                     <  1:141859/148‑1 (MQ=255)
                                                                 ggCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGTGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAg                                                                              <  1:186440/149‑1 (MQ=255)
                                                                    gagaAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGccc                                                                           >  4:101626/1‑149 (MQ=255)
                                                                                   ggAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCGCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCa                                                            >  8:39280/1‑149 (MQ=255)
                                                                                   ggAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGc                                                             <  8:68444/148‑1 (MQ=255)
                                                                                        ttACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCACCCTGATTGGCCCGCATCAcc                                                       >  8:202920/1‑149 (MQ=255)
                                                                                          aCAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGc                                                     >  3:124658/1‑149 (MQ=255)
                                                                                              cTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGCCCCTCGCGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTg                                                 >  8:99952/1‑149 (MQ=255)
                                                                                                   gCCTTAGGAATAAATAACCAGAATCAGATATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTAGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCTCCCTGATTGTCCCTCATCACCGCCCTGACCGc                                            <  6:245127/149‑1 (MQ=255)
                                                                                                   gCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCttt                                                                                                                                            >  7:296985/1‑53 (MQ=255)
                                                                                                   gCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCttt                                                                                                                                            <  8:296985/53‑1 (MQ=255)
                                                                                                        aGGAATAAATAACCAGAATCAGACATTTCCACTC‑TAGACCGGGTCTTTGAGTGGa                                                                                                                                     >  2:16374/1‑55 (MQ=255)
                                                                                                        aGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGa                                                                                                                                     <  1:16374/55‑1 (MQ=255)
                                                                                                            ataaataaCCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCAt                                   >  4:265465/1‑149 (MQ=255)
                                                                                                                ataaCCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATgcgc                               >  5:226916/1‑149 (MQ=255)
                                                                                                                   aCCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTgg                            <  7:295959/149‑1 (MQ=255)
                                                                                                                   aCCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTgg                            <  7:300536/149‑1 (MQ=255)
                                                                                                                        aaTCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTc                       <  6:111196/149‑1 (MQ=255)
                                                                                                                              aCATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCgg                 <  3:265465/149‑1 (MQ=255)
                                                                                                                               cATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCgg                 <  4:124658/148‑1 (MQ=255)
                                                                                                                                atatCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCGCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCa               >  5:255584/1‑149 (MQ=255)
                                                                                                                                   tCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGtt            >  7:82869/1‑149 (MQ=255)
                                                                                                                                     cactcaaaGACCCGGTCTAT‑AGTGGATATGTCGCTTATCAGCGGATCAAGGCACCCACACCATCGAGAAATCGAAGCAAAGCCCCCTGATTGGCCAGCATCACCGCCCTGCCCGACACACCCATGCGCTGTGCGTCCAGCGGCa               >  2:200706/8‑144 (MQ=255)
                                                                                                                                     cactcaaaGACCCGGTC‑TAGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCa               <  1:200706/137‑1 (MQ=255)
                                                                                                                                     cactcaaaGACCCGGTC‑TAGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGTTc           >  1:109026/8‑148 (MQ=255)
                                                                                                                                     cACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGccccc                                                                                                       >  3:269543/1‑56 (MQ=255)
                                                                                                                                     cACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGccccc                                                                                                       <  4:269543/56‑1 (MQ=255)
                                                                                                                                              aCCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAACGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGTTCCACCAggcg  >  4:136212/1‑149 (MQ=255)
                                                                                                                                                cGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCa                                                            >  3:58741/1‑89 (MQ=255)
                                                                                                                                                cGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCa                                                            <  4:58741/89‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
AAGCTTCCGACGCAAGCGTGTGACACTTCTGACTTTTAAAGTCTATTCGTAGGAAGTTTCCTAACGGCGAGAAATCCAGTGTAGGAAGTTACAACTTACGCCGTAGGAATAAATAACCAGAATCAGACATATCCACTC‑TAGACCGGGTCTTTGAGTGGATATGTCGGTTATCAGCGGATCAAGGCCCCCAGACCCTCGAGCAATCGCTGCAAAGCCCCCTGATTGGCCCGCATCACCGCCCTGCCCGCCTCACCCATGCGCTGGGCGTCCTGCGGCAGTTCCACCAGGCG  >  NC_002947/5608156‑5608445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑