Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,674,753	G→C	100%	intergenic (+115/+25)	rhlE‑II → / ← yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,674,753	0	G	C	100.0%	176.2 / NA	53	intergenic (+115/+25)	rhlE‑II/yceI	ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (28/25);  total (28/25)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

AGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑C‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674614‑5674890                                                                                                                                              |                                                                                                                                           aGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGt                                                                                                                                    >  6:297260/1‑149 (MQ=255)  gcgcTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGtt                                                                                                                                   <  5:261148/149‑1 (MQ=255)     ctgctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTc                                                                                                                                <  1:162327/149‑1 (MQ=255)        ctgACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGc                                                                                                                             >  2:263978/1‑149 (MQ=255)           aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTgg                                                                                                                          >  8:157790/1‑149 (MQ=255)             ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAGG‑CGGCCCCCGCGGTGTTCAGCTgg                                                                                                                          >  8:218247/1‑147 (MQ=37)             ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTgg                                                                                                                          >  3:276144/1‑147 (MQ=255)             ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTgg                                                                                                                          >  6:244864/1‑147 (MQ=255)               gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAg                                                                                                                       >  2:241266/1‑148 (MQ=255)                   gggCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAAtt                                                                                                                  <  1:49032/149‑1 (MQ=255)                              aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag                                                                                                       <  7:287235/149‑4 (MQ=25)                               gTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTca                                                                                                      >  5:280275/1‑149 (MQ=255)                                     aGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCtt                                                                                                >  7:55864/1‑149 (MQ=255)                                      gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                               <  5:297260/149‑1 (MQ=255)                                      gggggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTc                                                                                               <  4:284489/149‑1 (MQ=255)                                         ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGac                                                                                            <  6:100678/149‑1 (MQ=255)                                                           tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGTG‑GCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccag                                                                          >  4:149719/1‑149 (MQ=255)                                                           tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtcca                                                                           <  8:55864/148‑1 (MQ=255)                                                             aCGACACAAGGCCGCTCCTTCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                         <  7:321262/148‑1 (MQ=255)                                                             aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                         <  1:223472/148‑1 (MQ=255)                                                             aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                         <  5:212846/148‑1 (MQ=255)                                                             aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAggg                                                                        >  2:217545/1‑149 (MQ=255)                                                                 cacaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac                                                                                          >  8:191458/1‑127 (MQ=255)                                                                 cacaAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac                                                                                          <  7:191458/127‑1 (MQ=255)                                                                  agaaGGCCGCTCGTGCAGATAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                   <  4:173851/147‑1 (MQ=38)                                                                     aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGaa                                                                <  1:241266/149‑1 (MQ=255)                                                                       gCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGt                                                              <  7:157790/149‑1 (MQ=255)                                                                          gCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAg                                                            >  7:158392/1‑148 (MQ=255)                                                                            tCCTGCAGAGAATTTCTTGATCTCCACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag                                                                                                       <  5:314663/103‑4 (MQ=25)                                                                            tCCTGCAGAGAATTTCTTGATCTCCACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag                                                                                                       >  6:314663/1‑100 (MQ=25)                                                                              cTGCAGAGAATTTCTTGACCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCAccc                                                       >  3:107871/1‑149 (MQ=255)                                                                               tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGTCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                   <  5:286670/136‑1 (MQ=255)                                                                               tGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGc                                                                   >  6:286670/1‑136 (MQ=255)                                                                                 cAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg                                                      <  6:280275/147‑1 (MQ=255)                                                                                   gagaATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑Gcc                                                 >  6:29976/1‑149 (MQ=255)                                                                                          tCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGAt                                           <  1:263978/148‑1 (MQ=255)                                                                                             tGATCTCTACAGGAGCGGCCTTGTGTGGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAAATAATTCTGCTACACCCCATCGACACAGAAATCCAGGTCCAGGGTCTACGAAGTAGCACCCGG‑GCCTGTGATGcc                                        >  6:275124/1‑148 (MQ=255)                                                                                               acctctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGaa                                     <  4:107871/147‑1 (MQ=255)                                                                                                  tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAgac                                  >  2:254618/1‑147 (MQ=255)                                                                                                         gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCttt                                              <  8:36149/130‑1 (MQ=255)                                                                                                         gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCttt                                              >  7:36149/1‑130 (MQ=255)                                                                                                         gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAGTCgttcagg                           >  7:281934/1‑149 (MQ=255)                                                                                                             ggCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAGTCgttcaggtt                         >  2:47070/1‑147 (MQ=255)                                                                                                                   gtgtCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAGTCgttcag                            >  2:291382/1‑138 (MQ=255)                                                                                                                    tgtCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggt                >  1:276543/1‑149 (MQ=255)                                                                                                                          gATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAGTCCTTCAGGTTCAGCGTGGTGGTGGc          >  2:90398/1‑149 (MQ=255)                                                                                                                            tAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGt        <  5:29976/149‑1 (MQ=255)                                                                                                                            tAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGt        <  4:227083/149‑1 (MQ=255)                                                                                                                            tAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGt        <  2:276543/149‑1 (MQ=255)                                                                                                                              gggCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTGTGATGCCGAAGTCGGTCAGGTTCAGCGTGGTGGTGGCGtt       >  4:124505/1‑148 (MQ=255)                                                                                                                               ggCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTg      >  2:88113/1‑148 (MQ=255)                                                                                                                                 ccGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGCGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa    <  1:254618/149‑1 (MQ=255)                                                                                                                                  cGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc  >  7:294/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                           AGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑C‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG‑GCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674614‑5674890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑