| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,675,534 | T→C | 10.5% | D136G (GAC→GGC) ‡ | yceJ ← | iron‑regulated cytochrome b561 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,675,534 | 0 | T | C | 10.5% | 148.8 / 7.1 | 57 | D136G (GAC→GGC) ‡ | yceJ | iron‑regulated cytochrome b561 |
| Reads supporting (aligned to +/- strand): ref base T (29/22); new base C (4/2); total (33/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
TCAAGCTTTGCGGCCCAGCATGCGAGTCAGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGCCAGGTGGCCCAGCTTGGCCGCCAGGC > NC_002947/5675389‑5675681 | tCAAGCTTTGCGGCCCAGCATGCGAGTCAGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTcag > 7:270670/1‑149 (MQ=255) aaGCTTTGCGGCCCAGCATGCGAGTCAGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTcagg > 8:166657/1‑148 (MQ=255) aGCTTTGCGGCCCAGCATGCGAGTCAGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGt > 8:33712/1‑143 (MQ=255) aGCTTTGCGGCCCAGCATGCGAGTCAGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGt < 7:33712/143‑1 (MQ=255) aTGCGAGTCAGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGc > 6:18686/1‑149 (MQ=255) tGCGAGTCAGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGcc < 7:132160/149‑1 (MQ=255) tGCGAGTCAGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGcc < 3:158051/149‑1 (MQ=255) cGAGTCAGGGTCGCGTCACGGTCGATGAAATGATCCTTGAGCCCCGCAAGGGCATGCAGCACGGCAAAAATCACCCGCCCCCAAGCCAGCCACAAATCAACCCCCCCCGCCACAACTGCCTGGCCAGGCAGCTCGCTGATCACTGCCgg > 6:37997/1‑149 (MQ=255) tCAGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCAc > 7:164195/1‑148 (MQ=255) tCAGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGACcaggcagatgtg < 5:334635/132‑6 (MQ=255) tCAGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGACcaggcagatgtg > 6:334635/1‑127 (MQ=255) aGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTc < 5:18686/149‑1 (MQ=255) aGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTc < 8:164195/149‑1 (MQ=255) tCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTcagg > 8:193750/1‑118 (MQ=255) tCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTcagg < 7:193750/118‑1 (MQ=255) gTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGc < 7:166657/149‑1 (MQ=255) cGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGaa < 8:270670/149‑1 (MQ=255) ggTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGc < 3:103473/144‑1 (MQ=255) ggTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGc > 4:103473/1‑144 (MQ=255) gatgCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGcc > 6:284252/1‑148 (MQ=255) gCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAAt < 5:30723/142‑1 (MQ=255) gCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAAt > 6:30723/1‑142 (MQ=255) cgcgGCAAGGGGATGCAGCACGGCAAAAATCACCAGCCCCCACGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCTCTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTcgg < 5:37997/149‑1 (MQ=255) gcgGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGACcaggcagat < 4:40341/91‑3 (MQ=255) gcgGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGACcaggcagat > 3:40341/1‑89 (MQ=255) ggCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTcggcgg < 3:274387/149‑1 (MQ=255) cATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGGCGGCGGTGGAAATc < 5:284252/149‑1 (MQ=255) tGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAg < 6:349684/149‑1 (MQ=255) tGCAGCACGGCAAAAATCACAAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAAAAGGCCGAACACCGGAATGCCGACGCCGACGGCGGTGGAAAACAg > 7:278565/1‑149 (MQ=255) aaTCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAAcc > 6:13116/1‑148 (MQ=255) cAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGGCGTcggcattccg < 6:57748/123‑7 (MQ=255) cAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGGCGTcggcattccg > 5:57748/1‑117 (MQ=255) cccAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCaaa > 4:338004/1‑148 (MQ=255) cccAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCaaa > 1:5546/1‑148 (MQ=255) cccAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAAc < 3:338004/149‑1 (MQ=255) ccAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACa > 3:287681/1‑149 (MQ=255) aaGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGc < 2:5546/149‑1 (MQ=255) gccagccaCAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGcc > 2:328978/1‑148 (MQ=255) ccaCAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAgg > 6:82732/1‑148 (MQ=255) ccaCAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAgg > 4:21914/1‑148 (MQ=255) ccaCAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAgg > 7:38835/1‑148 (MQ=255) ccaCAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAgg > 7:33584/1‑148 (MQ=255) aaaTGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAg > 2:180589/1‑149 (MQ=255) aaTGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAgg > 3:338730/1‑149 (MQ=255) tGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGc > 1:232892/1‑149 (MQ=255) tGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGc < 5:13116/149‑1 (MQ=255) gAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGc > 2:314956/1‑148 (MQ=255) cacaCCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACAGCAAACAGCCCAAGGTACAGGGCCAGGt > 7:285070/1‑149 (MQ=255) cccGCGAAATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGCCAGGTGGcc < 7:264647/149‑1 (MQ=255) gaccTGCCTGACCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATTACCGCAAACAGCCCAAGGTACAGGGCCAGGTGGCCCAGCTTg > 2:173697/4‑149 (MQ=255) caTCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGCCAGGTGGCCCAGCTTg < 1:314956/149‑1 (MQ=255) aTCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGCCAGGTGGCCCAGCTTgg > 4:51788/1‑149 (MQ=255) aTCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGCCAGGTGGCCCAGCTTgg > 7:1872/1‑149 (MQ=255) gCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACCGCCCAAGGTACAGGGCCAGGAGGCCCAGCTTGgccgc > 6:20777/1‑149 (MQ=255) gCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGCCAGGTGGCCCAGCTTGgccgc > 4:63856/1‑149 (MQ=255) gCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGCCAGGTGGCCCAGCTTGgccgc > 4:89988/1‑149 (MQ=255) ccTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGCCAGGTGGCCCAGCTTGgc < 4:287681/145‑1 (MQ=255) cTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGCCAGGTGGCCCAGCTTGGCCGCCa < 3:89988/149‑1 (MQ=255) gTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGCCAGGTGGCCCAGCTTGGCCGCCAGGc < 2:232892/149‑1 (MQ=255) | TCAAGCTTTGCGGCCCAGCATGCGAGTCAGGGTCGCGTCACGGTCGATGAAATGATGCTTGAGCGCGGCAAGGGCATGCAGCACGGCAAAAATCACCAGCCCCCAAGCCAGCCACAAATGAACCACACCCGCCACATCTGCCTGGTCAGGCAGGTCGCTGATCAGTGCCGGCACTTCGAACAGGCCGAACACCGGAATGCCGACGCCGTCGGCGGTGGAAATCAGGTAACCGGCAACCATCACCGCAAACAGCCCAAGGTACAGGGCCAGGTGGCCCAGCTTGGCCGCCAGGC > NC_002947/5675389‑5675681 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |