Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,684,794	C→T	6.8%	S700N (AGC→AAC)	PP_4988 ←	signal transduction histidine kinase CheA/CheY‑like receiver

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,684,794	0	C	T	6.8%	175.0 / 2.5	59	S700N (AGC→AAC)	PP_4988	signal transduction histidine kinase CheA/CheY‑like receiver
Reads supporting (aligned to +/- strand):  ref base C (26/29);  new base T (2/2);  total (28/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CAGAGCGTCACGCTGGCCGGGCTGTTGCTGCCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCATTT  >  NC_002947/5684646‑5684941                                                                                                                                                      |                                                                                                                                                    cAGAGCGTCACGCTGGCCGGGCTGTTGCTGCCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGc                                                                                                                                                     >  4:200482/1‑149 (MQ=255) cAGAGCGTCACGCTGGCCGGGCTGTTGCTGCCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGc                                                                                                                                                     >  7:254302/1‑149 (MQ=255)                  cGGGCTGTTGCTGCCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTcc                                                                                                                                    <  2:108989/149‑1 (MQ=255)                  cGGGCTGTTGCTGCCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGCTcc                                                                                                                                    <  3:200482/149‑1 (MQ=255)                     gCTGTTGCTGCCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACg                                                                                                                                 >  3:215457/1‑149 (MQ=255)                      cTGTTGCTGCCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACgg                                                                                                                                <  4:335568/149‑1 (MQ=255)                       tGTTGCTGCCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGc                                                                                                                               <  4:7916/149‑1 (MQ=255)                          tgctgcCAGCGCGACAACAACGTCTCTGGCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGATGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGAAGGCt                                                                                                                                                    >  1:196035/1‑126 (MQ=255)                          tgctgcCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCt                                                                                                                                                    <  2:196035/125‑1 (MQ=255)                            ctgcCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGc                                                                                                                          >  6:126917/1‑149 (MQ=255)                             tgcCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGTTGGCCAGGCCGTGGACCGCCGCCTgg                                                                                                                           <  7:244608/147‑1 (MQ=255)                             tgcCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGTTGGCCAGGCCGTGGACCGCCGCCTgg                                                                                                                           >  8:244608/1‑147 (MQ=255)                              gcCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTgg                                                                                                                           <  3:242364/146‑1 (MQ=255)                              gcCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTgg                                                                                                                           >  4:242364/1‑146 (MQ=255)                                cAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAAc                                                                                                                      <  5:126917/149‑1 (MQ=255)                                 aGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAAcc                                                                                                                     >  2:192977/1‑149 (MQ=255)                                  gcgcGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCt                                                                                                                    <  8:254302/149‑1 (MQ=255)                                         aacaacGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGAt                                                                                                             <  4:93695/149‑1 (MQ=255)                                            aacGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGTTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAg                                                                                                          >  6:296342/1‑149 (MQ=255)                                               gTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGAt                                                                                                             <  2:181382/143‑1 (MQ=255)                                               gTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGAt                                                                                                             >  1:181382/1‑143 (MQ=255)                                                tctcT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTcc                                                                                                      >  4:279079/1‑149 (MQ=255)                                                       cATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCa                                                                                                >  4:203932/1‑149 (MQ=255)                                                        aTCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAAcc                                                                                                                     >  3:151486/1‑127 (MQ=255)                                                        aTCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAAcc                                                                                                                     <  4:151486/127‑1 (MQ=255)                                                        aTCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCAc                                                                                               >  3:236431/1‑149 (MQ=255)                                                         tCGAGCAGGATGTCCATGGCATCGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTAGCTGCGGCCCGCATGGTGCCGCTCTTCATGCAGCCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTACCGCGGCAcc                                                                                              >  4:239260/1‑149 (MQ=255)                                                           gAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCg                                                                                             <  4:236431/148‑1 (MQ=255)                                                                      ccATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATggcggtg                                                                                 >  1:294590/1‑147 (MQ=255)                                                                       catggcatggGCCAGGAAGCTGGCGACCCACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCaca                                                                                                                      >  6:292045/1‑109 (MQ=255)                                                                       catggcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCa                                                                                <  8:68673/149‑1 (MQ=255)                                                                           gcatggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAggg                                                                             <  6:26472/148‑1 (MQ=255)                                                                             atggGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCg                                                                          >  2:124282/1‑149 (MQ=255)                                                                                    aGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTCGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATc                                                                   >  8:103414/1‑149 (MQ=255)                                                                                      gAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGa                                                                 <  8:111577/149‑1 (MQ=255)                                                                                              cGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTgcgc                                                          >  1:56216/1‑148 (MQ=255)                                                                                               gACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGt                                                               <  7:131071/142‑1 (MQ=255)                                                                                               gACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGt                                                               >  8:131071/1‑142 (MQ=255)                                                                                               gACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGcc                                                         >  8:224972/1‑148 (MQ=255)                                                                                                aCCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCaa                                                       <  1:124282/149‑1 (MQ=255)                                                                                                  cAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGc                                                     <  2:294590/149‑1 (MQ=255)                                                                                                     cTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCTACCTGCTCGATCAGTTCCGCGGCACCg                                                                                             <  1:185638/106‑1 (MQ=255)                                                                                                     cTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCg                                                                                             >  2:185638/1‑106 (MQ=255)                                                                                                        cGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGa                                                <  3:203932/148‑1 (MQ=255)                                                                                                        cGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGAt                                               <  1:192977/149‑1 (MQ=255)                                                                                                         ggATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATg                                              <  2:490/149‑1 (MQ=255)                                                                                                          gATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGcc                                                    <  6:206581/142‑1 (MQ=255)                                                                                                          gATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGcc                                                    >  5:206581/1‑142 (MQ=255)                                                                                                          gATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATgg                                             <  7:103414/149‑1 (MQ=255)                                                                                                          gATCACGCTAGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATg                                              >  4:167121/1‑148 (MQ=255)                                                                                                                 cTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGaa                                           <  7:180110/144‑1 (MQ=255)                                                                                                                 cTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGaa                                           >  8:180110/1‑144 (MQ=255)                                                                                                                         ggccggcATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCa                              <  7:224972/149‑1 (MQ=255)                                                                                                                         ggccggcATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCa                              <  2:56216/149‑1 (MQ=255)                                                                                                                                  ggTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGc                     >  1:260492/1‑149 (MQ=255)                                                                                                                                        ggTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGccattc                >  2:95101/1‑148 (MQ=255)                                                                                                                                              cATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCAttt           <  2:260492/147‑1 (MQ=255)                                                                                                                                              cATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTc          <  3:167121/148‑1 (MQ=255)                                                                                                                                               aTGCAGGTTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCg         <  5:296342/148‑1 (MQ=255)                                                                                                                                                   aGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCAt    >  8:266724/1‑149 (MQ=255)                                                                                                                                                     gCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCAttt  >  5:52455/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CAGAGCGTCACGCTGGCCGGGCTGTTGCTGCCAGCGCGACAACAACGTCTCT‑GCATCGAGCAGGATGTCCATGGCATGGGCCAGGAAGCTGGCGACCAACTGCGGATCACGCTTGCTGCGGCCGGCATGGTGCGGGTCTTCATGCAGGCTGGCCAGGCGGCGGTCCACGGCCTGGCCAACCTGCTCGATCAGTTCCGCGGCACCGGGGATGGCGGCCAGGGGCGTGCTATCGAGTTGCGCCAAGCCCAGATGGAACAACGACCGCGCAGCCTCCAGCCATTCCATTTCGGCCATTT  >  NC_002947/5684646‑5684941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑