Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,740,474 C→A 26.7% intergenic (+39/+32) hutF → / ← PP_5037 formiminoglutamate deiminase/lipocalin family lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,740,4740CA26.7% 93.8 / 35.3 56intergenic (+39/+32)hutF/PP_5037formiminoglutamate deiminase/lipocalin family lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base A (6/9);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

ATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAG  >  NC_002947/5740332‑5740616
                                                                                                                                               |                                                                                                                                              
aTGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGaa                                                                                                                                          <  2:318465/149‑1 (MQ=255)
 tgtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAg                                                                                                                                         <  3:134779/149‑1 (MQ=255)
 tgtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAACCCTTCGCGGGCTTGCATGCGAAg                                                                                                                                         <  2:276062/149‑1 (MQ=39)
  gtgATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGTGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAACCCTTCGCGGGCTTGCACGCGAAg                                                                                                                                         <  2:6388/148‑1 (MQ=39)
     aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGtt                                                                                                                                     <  3:254551/149‑1 (MQ=255)
     aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGt                                                                                                                                      <  6:123274/148‑1 (MQ=255)
     aTGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAACCCTTCGCGGGCTTGCACGCGAAgggc                                                                                                                                      <  2:98698/148‑2 (MQ=38)
      tGGTGAATGGGCAATGGGGGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAACCCTTCGCGGGCTTGCACGCGAAGGGCTg                                                                                                                                    <  6:290079/149‑1 (MQ=38)
              gggCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGGGCTTGCACGCGAAg                                                                                                                                         <  6:66378/136‑1 (MQ=255)
              gggCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGGGCTTGCACGCGAAg                                                                                                                                         >  5:66378/1‑136 (MQ=255)
                         ggtgCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTTGGCGTTGGGGCCCTAGGGGGGGACGCCCCGCACGGGTTGGACCGCCACCCCcaaaa                                                                                                                   >  1:125982/1‑148 (MQ=255)
                            gcgcCTGGGGGGGCTTGCCAAGTATTATTATCGGGGGCGGGCTTTTCCAATGGTGTTGGGCAAGCTTTTGGTCTGTGTTTAGGGGGTTT‑GGGTTCCAGCCCTTGGCGTGCAAGCCGGCTATGGGTTCAAACGCAACCCCAAAAAACTCa                                                                                                              <  4:139346/149‑1 (MQ=255)
                                cAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTg                                                                                                                                    >  7:12027/1‑123 (MQ=255)
                                cAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTg                                                                                                                                    <  8:12027/123‑1 (MQ=255)
                                           tGCCCAGGAATATGACAGCGGGCGGGCGTTTGCAAAGGTGTTGCGCCAGCTTTTGGGCTGCGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCTAAGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTAt                                                                                               <  5:294683/149‑1 (MQ=255)
                                                aGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTAGCGTGGAAGCCCGCGAAGGGTTTGAACGCAACCCCAAAAAAATCAGTGCATTTTTACTAttttgt                                                                                          >  5:61143/1‑146 (MQ=255)
                                                       tGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGATTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCATCCCTTCGCTTGAAAGCCCGCTAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGa                                                                                   <  5:181877/149‑1 (MQ=255)
                                                        gAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAg                                                                                                                                         <  5:210747/94‑1 (MQ=255)
                                                        gAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAg                                                                                                                                         >  6:210747/1‑94 (MQ=255)
                                                              gggcgggcGTTTGCCTAGGGGTTGCTCCTGCTTTTGGGCTGAGCTTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCACGCCGGCGATGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGAcgcccgc                                                                            <  6:126466/149‑1 (MQ=255)
                                                                    gcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAACCCTTCGCGGGCTTGCACGCGAAggg                                                                                                                                       <  1:127531/84‑1 (MQ=21)
                                                                    gcgTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAACCCTTCGCGGGCTTGCACGCGAAggg                                                                                                                                       >  2:127531/1‑84 (MQ=21)
                                                                      gTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTTCAGCCCTTAGCGTGCAAGCCCCCGAAGGGTTGGAACGCAACCCCAAAAAACAGAGGGGATTTTTAATATTTGCTGAAAGGACGCCCCCCCGATCaa                                                                    >  6:158771/1‑149 (MQ=255)
                                                                            cAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGGTTCAGCCCATAGCGGGGAAGCCCGCGAACGGTTTGAACCCAAACCCCAAAAAAAAACGGCATTTTTTATATTTTGTGAAAGGCCCCCCGCCACAGAAAGCGGcg                                                              >  2:208472/1‑148 (MQ=255)
                                                                             aaGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTg                                                             <  1:208472/149‑1 (MQ=255)
                                                                                  gtTGCGCCAGCTTTTGGGCTGAGCGTACCGNGGTT‑GCGTTGCAGCCCTTCGCGTGGAAGCCCGCGAAGGGTTGGGACGCAACCCCAAAAAACTCAGTGCATTTTTAGTATTTGGTGATGGGGCGCCCGCCCGATGAAGCGGgtggtgg                                                         >  1:27501/1‑147 (MQ=255)
                                                                                      cgcCTGCTTTTGGGGTCCGCGACCCGGGGTG‑GCGTGGCAGCCTTCCGCGTCCACGCCCGATAAGGGTTCAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAc                                                    <  2:325968/149‑1 (MQ=255)
                                                                                      cgcCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAc                                                    <  2:100901/149‑1 (MQ=255)
                                                                                        ccAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATAccc                                                  <  5:158771/149‑1 (MQ=255)
                                                                                                          gTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGa                                <  8:208215/149‑1 (MQ=255)
                                                                                                                   tt‑gcgttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGtt                       >  5:78056/1‑149 (MQ=255)
                                                                                                                        gttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                   <  6:78056/149‑1 (MQ=255)
                                                                                                                        gttgcAACCCTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                   >  7:137137/1‑149 (MQ=38)
                                                                                                                        gttgcAACCCTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCa                   >  6:187726/1‑149 (MQ=38)
                                                                                                                         ttgcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                  >  4:174085/1‑149 (MQ=255)
                                                                                                                           gcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcag                             >  3:312109/1‑136 (MQ=255)
                                                                                                                           gcAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGAcag                             <  4:312109/136‑1 (MQ=255)
                                                                                                                            caacccTTCGCGGGCTTGCACGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                  <  2:214526/143‑1 (MQ=39)
                                                                                                                            caacccTTCGCGGGCTTGCACGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCAc                  >  1:214526/4‑146 (MQ=39)
                                                                                                                             aacccTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGa                                                                        >  5:165934/3‑91 (MQ=21)
                                                                                                                             aacccTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGa                                                                        <  6:165934/89‑1 (MQ=21)
                                                                                                                             aGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAgg                         <  5:206733/138‑1 (MQ=255)
                                                                                                                             aGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAgg                         >  6:206733/1‑138 (MQ=255)
                                                                                                                             aGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTc              >  6:13583/1‑149 (MQ=255)
                                                                                                                              gCCCTTCGCGTGCAAGCCCGCTATGGGTTGAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTcc             <  1:324200/149‑1 (MQ=255)
                                                                                                                               cccTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCCACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCTGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCGcc             >  6:39569/1‑148 (MQ=255)
                                                                                                                               cccTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                                  >  4:296459/1‑127 (MQ=255)
                                                                                                                               cccTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTg                                  <  3:296459/127‑1 (MQ=255)
                                                                                                                                 cTTCGCGGGCTTGCACGCGAAGGGCTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAg          <  8:137137/149‑1 (MQ=39)
                                                                                                                                      cgTGCATCCCCGCTATGGGTTCAAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCgg     <  4:175931/149‑1 (MQ=255)
                                                                                                                                         gCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAg  >  6:74912/1‑149 (MQ=255)
                                                                                                                                         gCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAg  >  5:123401/1‑149 (MQ=255)
                                                                                                                                           aaGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgctg                                            >  7:302256/1‑105 (MQ=255)
                                                                                                                                           aaGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCctgctg                                            <  8:302256/105‑1 (MQ=255)
                                                                                                                                           aaGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCa   >  5:71257/1‑146 (MQ=255)
                                                                                                                                           aaGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCa   <  6:71257/146‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
ATGTGATGGTGAATGGGCAATGGGTGGTGCGCCAGGGGCGGCATGCCCAGGAAGATGAAAGCGGGCGGGCGTTTGCCAAGGTGTTGCGCCAGCTTTTGGGCTGAGCGTACCGGGGTT‑GCGTTGCAGCCCTTCGCGTGCAAGCCCGCGAAGGGTTGCAACGCAACCCCAAAAAACTCAGTGCATTTTTACTATTTGCTGATCGGACGCCCGCCAGATCAAGCGGCTGGTGTCATACCCCTGCTGCCGCGCCCTGGACAGCAGGTTCTCACGCTCCCAGGCCGGCAG  >  NC_002947/5740332‑5740616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑