Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,776,703 C→T 6.9% G340G (GGC→GGT)  betA‑II → choline dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,776,7030CT 6.9% 214.1 / 3.9 72G340G (GGC→GGT) betA‑IIcholine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (30/37);  new base T (3/2);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.36e-01

CGGGCGTTGGTGAAAACCTTCAGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGAAGGAACATGGCTTCCAGGCGCACATGGG  >  NC_002947/5776558‑5776849
                                                                                                                                                 |                                                                                                                                                  
cGGGCGTTGGTGAAAACCTTCAGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGgcgc                                                                                                                                                  <  2:299846/148‑1 (MQ=255)
 gggCGTTGGTGAAAACCTTCAGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCa                                                                                                                                                <  2:148300/149‑1 (MQ=255)
      ttGGTGAAAACCTTCAGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAAc                                                                                                                                           <  1:5766/149‑1 (MQ=255)
        ggTGAAAACCTTCAGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAAc                                                                                                                                           >  4:160199/1‑147 (MQ=255)
            aaaaCCTTCAGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTc                                                                                                                                     >  2:230696/1‑149 (MQ=255)
            aaaaCCTTCAGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGAGGATGTTCAATGGCACCGGTATCGGTGCCAGCAACCAGTTc                                                                                                                                     >  1:288167/1‑149 (MQ=255)
               aCCTTCAGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAg                                                                                                                                  >  8:131572/1‑149 (MQ=255)
               aCCTTCAGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAg                                                                                                                                  <  5:43014/149‑1 (MQ=255)
                 cTTCAGGACCACCTGTAACTGTACCTTCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCTATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCAAGGc                                                                                                                                <  8:171199/149‑1 (MQ=255)
                 cTTCAGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGc                                                                                                                                >  1:228307/1‑149 (MQ=255)
                  ttCAGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGcc                                                                                                                               >  5:283901/1‑149 (MQ=255)
                     aGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCggg                                                                                                                            <  4:337287/149‑1 (MQ=255)
                       gACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCGCCGATACCGGTGCCa                                                                                                                                                >  2:56664/1‑127 (MQ=255)
                       gACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCGCCGATACCGGTGCCa                                                                                                                                                <  1:56664/127‑1 (MQ=255)
                       gACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCggggg                                                                                                                          >  5:135291/1‑149 (MQ=255)
                        accaccTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCgggggg                                                                                                                         <  6:283901/149‑1 (MQ=255)
                         ccaccTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGt                                                                                                                        <  7:254768/149‑1 (MQ=255)
                          caccTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGtt                                                                                                                       <  2:227131/149‑1 (MQ=255)
                            ccTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCa                                                                                                                     >  8:26999/1‑149 (MQ=255)
                              tGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATc                                                                                                                   >  1:181246/1‑149 (MQ=255)
                                   cTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGa                                                                                                                                   <  8:153042/128‑1 (MQ=255)
                                   cTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGa                                                                                                                                   >  7:153042/1‑128 (MQ=255)
                                      tACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTc                                                                                                            <  6:122382/148‑1 (MQ=255)
                                            cAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGa                                                                                                     <  7:253195/149‑1 (MQ=255)
                                                  gCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCaa                                                                                               <  2:267104/149‑1 (MQ=255)
                                                  gCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCaa                                                                                               <  3:160199/149‑1 (MQ=255)
                                                    cTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGgc                                                                                                                                                    <  5:151325/94‑1 (MQ=255)
                                                    cTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGgc                                                                                                                                                    >  6:151325/1‑94 (MQ=255)
                                                    cTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGt                                                                                             >  7:95008/1‑149 (MQ=255)
                                                    cTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGt                                                                                             <  6:292615/149‑1 (MQ=255)
                                                    cTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGt                                                                                             >  1:303731/1‑149 (MQ=255)
                                                         cccAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGcc                                                                                         >  2:219370/1‑148 (MQ=255)
                                                          ccAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCg                                                                                        >  4:74395/1‑148 (MQ=255)
                                                          ccAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCg                                                                                        >  5:22738/1‑148 (MQ=255)
                                                            aGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGa                                                                                                               <  2:329674/123‑1 (MQ=255)
                                                            aGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGa                                                                                                               >  1:329674/1‑123 (MQ=255)
                                                              ccGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACAt                                                                                   <  2:228307/149‑1 (MQ=255)
                                                               cGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGt                                                                                             >  8:87778/1‑138 (MQ=255)
                                                               cGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGt                                                                                             <  7:87778/138‑1 (MQ=255)
                                                               cGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATc                                                                                  <  6:135291/149‑1 (MQ=255)
                                                               cGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATc                                                                                  <  2:142925/149‑1 (MQ=255)
                                                                ggTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATcc                                                                                 <  2:303731/149‑1 (MQ=255)
                                                                     cGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATc                                                                                                                   >  8:242490/1‑110 (MQ=255)
                                                                     cGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATc                                                                                                                   <  7:242490/110‑1 (MQ=255)
                                                                             ccGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCt                                                                    >  3:76368/1‑149 (MQ=255)
                                                                             ccGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCt                                                                    <  7:26999/149‑1 (MQ=255)
                                                                                   ctgctgTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATc                                                                                  >  4:173445/1‑129 (MQ=255)
                                                                                   ctgctgTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATc                                                                                  <  3:173445/129‑1 (MQ=255)
                                                                                      ctgTGTTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACGAGTTCGAGGCCGGGGGGTTCATCCGGTCTCGTCCGGAGTTCAAGTGGCCGAACATGCAGTACCACTTCCTGCCGGTTGc                                                           <  2:291643/149‑1 (MQ=255)
                                                                                       tgtgGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGc                                                           >  8:219232/1‑148 (MQ=255)
                                                                                         tggtggAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGTGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGa                                                         <  2:288167/148‑1 (MQ=255)
                                                                                         tggtggAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGAt                                                        <  2:181246/149‑1 (MQ=255)
                                                                                           gtggAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGAtt                                                       >  3:312960/1‑148 (MQ=255)
                                                                                              gAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCg                                                                <  1:164003/136‑1 (MQ=255)
                                                                                              gAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCg                                                                >  2:164003/1‑136 (MQ=255)
                                                                                                   aGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAAc                                              >  8:85571/1‑149 (MQ=255)
                                                                                                          gATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGTGATTAACTACAACGGCTcc                                        >  8:288495/1‑148 (MQ=255)
                                                                                                          gATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGAt                                                        >  3:209150/1‑132 (MQ=255)
                                                                                                          gATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGAt                                                        <  4:209150/132‑1 (MQ=255)
                                                                                                               gTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGc                                  <  4:312960/149‑1 (MQ=255)
                                                                                                                tGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCg                                 >  1:138996/1‑149 (MQ=255)
                                                                                                                  ccGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGCTTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGtt                                                             <  1:258690/119‑1 (MQ=255)
                                                                                                                  ccGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGtt                                                             >  2:258690/1‑119 (MQ=255)
                                                                                                                    gAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGAt                                                        >  5:262933/1‑122 (MQ=255)
                                                                                                                    gAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGAt                                                        <  6:262933/122‑1 (MQ=255)
                                                                                                                    gAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGaa                             <  4:76368/149‑1 (MQ=255)
                                                                                                                    gAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGaa                             <  7:131572/149‑1 (MQ=255)
                                                                                                                         gATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGAAGGAAc                        <  8:95008/149‑1 (MQ=255)
                                                                                                                            gTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGTAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGAAGGAACAt                      <  7:219232/148‑1 (MQ=255)
                                                                                                                              ttgctGGCGCCGATACCGGTGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGAAGGAACATGGc                   >  4:123447/5‑149 (MQ=255)
                                                                                                                               cAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGAAGGAACATGGCt                  >  8:165103/1‑149 (MQ=255)
                                                                                                                                aaTGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGAAGGAACATGGCtt                 >  3:9550/1‑149 (MQ=255)
                                                                                                                                      aCCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGAAGGAACATGGCTTCCAGgc           <  6:22738/149‑1 (MQ=255)
                                                                                                                                             tCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGAAGGAACATGGCTTCCAGGCGCACAt     <  1:219370/148‑1 (MQ=255)
                                                                                                                                               ggCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGAAGGAACATGGCTTCCAGGCGCACATggg  <  7:165103/149‑1 (MQ=255)
                                                                                                                                                gcgcCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGAAgg                           >  8:146659/1‑123 (MQ=255)
                                                                                                                                                gcgcCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGAAgg                           <  7:146659/123‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGGGCGTTGGTGAAAACCTTCAGGACCACCTGGAACTGTACCTGCAGTACGCCTGCACCCAGCCGGTTTCGCTATACCCGTCGCTGCTGTGGTGGAACCAGCCGGCGATCGGTGCCGAGTGGATGTTCAATGGCACCGGTATCGGCGCCAGCAACCAGTTCGAGGCCGGGGGGTTCATCCGGACTCGTCCGGAGTTCAAGTGGCCGAACATCCAGTACCACTTCCTGCCGGTTGCGATTAACTACAACGGCTCCAATGGCGTGAAGGAACATGGCTTCCAGGCGCACATGGG  >  NC_002947/5776558‑5776849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑