Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,786,991	T→G	5.4%	L135R (CTG→CGG)	trxC →	thioredoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,786,991	0	T	G	5.4%	236.8 / 3.0	74	L135R (CTG→CGG)	trxC	thioredoxin
Reads supporting (aligned to +/- strand):  ref base T (29/41);  new base G (2/2);  total (31/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

CTGCCGCCTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCATTTTCACCGAG  >  NC_002947/5786851‑5787136                                                                                                                                             |                                                                                                                                                  cTGCCGCCTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGt                                                                                                                                           <  1:139287/149‑1 (MQ=255)   gccgccTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTgg                                                                                                                                         <  3:83437/149‑1 (MQ=255)    ccgccTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAg                                                                                                                                            <  3:83007/145‑1 (MQ=255)    ccgccTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAg                                                                                                                                            >  4:83007/1‑145 (MQ=255)    ccgccTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGCAGCGACTGGAGTgg                                                                                                                                         <  8:115924/148‑1 (MQ=255)     cgccTGGCCAAGCTCGACAGCGAAGCCAACCGTAACATCGCAGGGCAAATGGTCATACCCTCGATCACCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCCCCAGGCCGGCCCGTTTCCACTCCAGTCGCTGCTGGAGTGGCt                                                                                                                                       >  8:317070/1‑149 (MQ=255)       ccTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGTGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGc                                                                                                                                     <  2:275419/149‑1 (MQ=255)       ccTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGTCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGc                                                                                                                                     <  1:215347/149‑1 (MQ=255)             cAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGCAGCGACTGGAGTggaa                                                                                                                                       <  6:255891/141‑3 (MQ=255)             cAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGCAGCGACTGGAGTggaa                                                                                                                                       >  5:255891/1‑139 (MQ=255)                    gACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTg                                                                                                                                          >  6:339122/1‑131 (MQ=255)                    gACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTg                                                                                                                                          <  5:339122/131‑1 (MQ=255)                      cAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGttt                                                                                                                      <  3:131856/149‑1 (MQ=255)                       aGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTa                                                                                                                     <  4:38572/149‑1 (MQ=255)                        gCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTaa                                                                                                                    <  1:237072/149‑1 (MQ=255)                        gCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTaa                                                                                                                    >  3:52940/1‑149 (MQ=255)                          gAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGc                                                                                                                  >  1:266035/1‑149 (MQ=255)                          gAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGc                                                                                                                  >  5:250501/1‑149 (MQ=255)                           aaGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGttt                                                                                                                      <  6:129934/144‑1 (MQ=255)                           aaGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGttt                                                                                                                      >  5:129934/1‑144 (MQ=255)                             gCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGtt                                                                                                               <  4:52940/149‑1 (MQ=255)                               cAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGtttt                                                                                                             <  4:244732/149‑1 (MQ=255)                               cAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGttt                                                                                                              >  3:244732/1‑148 (MQ=255)                                    gTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCa                                                                                                        <  5:186204/149‑1 (MQ=255)                                         cTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAagcag                                                                                                   <  1:334959/149‑1 (MQ=255)                                             cAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGaa                                                                                               <  2:266035/149‑1 (MQ=255)                                              aGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAg                                                                                                                            <  5:268878/119‑1 (MQ=255)                                              aGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAg                                                                                                                            >  6:268878/1‑119 (MQ=255)                                               gggCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATg                                                                                             <  6:250501/149‑1 (MQ=255)                                                ggCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGc                                                                                            <  7:317070/149‑1 (MQ=255)                                                 gCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCa                                                                                           <  1:27154/149‑1 (MQ=255)                                                                   gATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGa                                                                          <  6:77374/148‑1 (MQ=255)                                                                      ccccggcctggcggctgatttcgcggccgttcttgaacagcagcaggctggggCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGTTGAGt                                                                        >  5:283265‑M2/54‑147 (MQ=255)                                                                       CCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAg                                                                     <  7:162920‑M1/149‑1 (MQ=255)                                                                       CCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAg                                                                     <  3:262117‑M1/149‑1 (MQ=255)                                                                        CCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGc                                                                    <  4:199907‑M1/149‑1 (MQ=255)                                                                        CCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGc                                                                    <  8:203116‑M1/149‑1 (MQ=255)                                                                         cggcctggcggctgatttcgcggccgttcttgaacagcagcaggctggggCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGa                                                                          >  3:88428‑M2/51‑142 (MQ=255)                                                                         cggcctggcggctgatttcgcggccgttcttgaacagcagcaggctggggCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGa                                                                          <  4:88428‑M2/92‑1 (MQ=255)                                                                            ccTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTtgt                                                                <  8:162920/149‑1 (MQ=255)                                                                              tgctgctgTTCAAGAACGGCCGCTAAATCAGCGGCCAGTCGGGGGCTTTTCCTCTCCAGTCGCTGCTTGAGTGGCTGCGTAGCCAAGGGGTTTATGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGc                                                              <  2:79997/149‑1 (MQ=255)                                                                              tgctgctgTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGc                                                                    >  8:33486/1‑143 (MQ=255)                                                                              tgctgctgTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGc                                                                    <  7:33486/143‑1 (MQ=255)                                                                                   ctgTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCgg                                                         >  2:125305/1‑149 (MQ=255)                                                                                      ttCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTc                                                                       >  8:212330/1‑132 (MQ=255)                                                                                      ttCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTc                                                                       <  7:212330/132‑1 (MQ=255)                                                                                      ttCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGc                                                        <  4:262117/147‑1 (MQ=255)                                                                                      ttCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGc                                                        <  5:282420/147‑1 (MQ=255)                                                                                        cAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCa                                                    >  4:4392/1‑149 (MQ=255)                                                                                          aGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAgg                                                  <  3:4392/149‑1 (MQ=255)                                                                                           gAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGt                                                 <  1:197962/149‑1 (MQ=255)                                                                                                  cgcgAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGt                                                 <  8:211518/142‑1 (MQ=255)                                                                                                  cgcgAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGt                                                 >  7:211518/1‑142 (MQ=255)                                                                                                         tCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAgg                                   >  7:244581/1‑149 (MQ=255)                                                                                                           aGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTcc                                                     <  7:175592/129‑1 (MQ=255)                                                                                                           aGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTcc                                                     >  8:175592/1‑129 (MQ=255)                                                                                                            gccgccAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTg                                >  6:59534/1‑149 (MQ=255)                                                                                                               gccAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGa                                                                          <  4:153680/104‑1 (MQ=255)                                                                                                               gccAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGa                                                                          >  3:153680/1‑104 (MQ=255)                                                                                                               gccAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCg                             >  4:262389/1‑149 (MQ=255)                                                                                                                 caggctggggCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGTTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCgtg                           <  6:283265‑M2/139‑1 (MQ=255)                                                                                                                   ggCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCgtggt                         >  8:256224/1‑149 (MQ=255)                                                                                                                    gCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCgtggtg                        >  8:191922/1‑149 (MQ=255)                                                                                                                          gCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGa                                               >  8:122655‑M2/2‑120 (MQ=255)                                                                                                                          gCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGa                                               <  7:122655‑M2/119‑1 (MQ=255)                                                                                                                          gCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTc                  <  3:199907‑M2/148‑1 (MQ=255)                                                                                                                          gCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTc                  <  8:244581‑M2/148‑1 (MQ=255)                                                                                                                            gTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTcgc                >  7:297631/1‑149 (MQ=255)                                                                                                                              agccACTCCAGCAGCGACTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTcgcg               >  4:8124/3‑148 (MQ=255)                                                                                                                               tCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCa             >  7:19432/1‑149 (MQ=255)                                                                                                                                ccACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCg                      <  2:326181/139‑1 (MQ=255)                                                                                                                                ccACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCg                      >  1:326181/1‑139 (MQ=255)                                                                                                                                 cACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCAtt           >  3:288261/1‑149 (MQ=255)                                                                                                                                  aCTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCATGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCAttt          >  4:96006/1‑149 (MQ=255)                                                                                                                                           gctgctGGAGTGGCTGCGTAGCCATGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCATTTTCACCGAg  <  3:96006/148‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                  CTGCCGCCTGGCCAAGCTCGACAGCGAAGCCAACCGTAACCTCGCAGGGCAACTGGGCATACGCTCGATCCCCAGCCTGCTGCTGTTCAAGAACGGCCGCGAAATCAGCCGCCAGGCCGGGGCGTTTCCACTCCAGTCGCTGCTGGAGTGGCTGCGTAGCCAAGGGGTTTAAGCGTTTTCTTCAAGCAGCGAATGCAGCTCGACAAACTGCTGAGTCAGCTTGTGCCGCGGCTCCAGGTGAATCAGCGGCAGGCTGGCGTGGTGCGATTCGCGCATTTTCACCGAG  >  NC_002947/5786851‑5787136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑