Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 487,825 G→C 6.3% R159R (CGC→CGG)  pdxA ← 4‑hydroxythreonine‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947487,8250GC 6.3% 199.8 / 3.8 63R159R (CGC→CGG) pdxA4‑hydroxythreonine‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (29/30);  new base C (2/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

GGCCGCACACGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGC  >  NC_002947/487680‑487969
                                                                                                                                                 |                                                                                                                                                
ggCCGCACACGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCAcgcg                                                                                                                                                >  3:13916/1‑148 (MQ=255)
 gCCGCACACGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCa                                                                                                                                              <  1:61989/149‑1 (MQ=255)
    gCACACGAGGATACTCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGc                                                                                                                                           >  1:310591/1‑149 (MQ=255)
         cGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCgg                                                                                                                                      <  6:160083/149‑1 (MQ=255)
          gAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCggg                                                                                                                                     >  3:198661/1‑149 (MQ=255)
            ggATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTg                                                                                                                                   <  4:145603/149‑1 (MQ=255)
             gATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACCCGCGGCCTGCGCGTgg                                                                                                                                  <  4:309083/149‑1 (MQ=255)
                  gcgGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAg                                                                                                                              >  6:144357/1‑148 (MQ=255)
                  gcgGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACCCGCGGCCTGCGCGTGGc                                                                                                                                 >  6:82856/1‑145 (MQ=255)
                  gcgGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACCCGCGGCCTGCGCGTGGc                                                                                                                                 <  5:82856/145‑1 (MQ=255)
                  gcgGGTTGGCAATGCCAAACTTGGCGCGCATGTCGGCATGCAGGATACGGTTTACCCGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCatg                                                                                                                                            <  7:220881/134‑3 (MQ=255)
                         ggCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCAtt                                                                                                                       <  8:158868/148‑1 (MQ=255)
                         ggCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCAtt                                                                                                                       <  4:279715/148‑1 (MQ=255)
                            aaTGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCa                                                                                                                   <  3:185218/149‑1 (MQ=255)
                            aaTGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCa                                                                                                                   <  6:221685/149‑1 (MQ=255)
                             aTGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCAc                                                                                                                  <  7:301484/149‑1 (MQ=255)
                                ccAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCTGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtg                                                                                                               <  4:13916/149‑1 (MQ=255)
                                 cAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCGGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtgt                                                                                                              >  5:115597/1‑149 (MQ=255)
                                     cTTGTCGCGCATGTCGGCATGCAGGATAGGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTgtgcgg                                                                                                          >  1:196888/1‑149 (MQ=255)
                                           gcgcATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCCTCATTGCCACTTGTGCGGTGCggg                                                                                                    >  4:96976/1‑149 (MQ=255)
                                           gcgcATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGtgcggtgcgg                                                                                                     <  7:314980/148‑1 (MQ=255)
                                           gcgcATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGtgcggtgcgg                                                                                                     <  2:196888/148‑1 (MQ=255)
                                               aTGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtgtg                                                                                                             <  4:68616/136‑1 (MQ=255)
                                               aTGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtgtg                                                                                                             >  3:68616/1‑136 (MQ=255)
                                                   cGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCg                                                                                            >  5:157567/1‑149 (MQ=255)
                                                     gCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCgg                                                                                           >  3:293091/1‑148 (MQ=255)
                                                        tGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAg                                                                                       <  5:144357/149‑1 (MQ=255)
                                                          cAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGa                                                                                     <  4:198661/149‑1 (MQ=255)
                                                            ggATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATAATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAAt                                                                                   >  3:60448/1‑149 (MQ=255)
                                                             gATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACCTCACGCAGTGGCCGGTGCGTGGTCACCAGGGCCCAGCGCCGGCCGGGGGTGGCCAGGCTCATTGGCCCTGGTGGGGTGCGGGGCCGGTCGGCAAGGagt                                                                                   >  6:345289/1‑146 (MQ=255)
                                                                  gggTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTa                                                                             >  7:241377/1‑149 (MQ=255)
                                                                     ttACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGc                                                                          >  5:265571/1‑149 (MQ=255)
                                                                         acGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCgg                                                                      <  2:310591/149‑1 (MQ=255)
                                                                         acGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCg                                                                       >  5:155246/1‑148 (MQ=255)
                                                                                aCGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACGAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGt                                                                              <  5:4742/134‑1 (MQ=255)
                                                                                aCGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCACGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGt                                                                              >  6:4742/1‑134 (MQ=255)
                                                                                  gcgcTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGa                                                             <  6:155246/149‑1 (MQ=255)
                                                                                        tGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGc                                                       >  4:293809/1‑149 (MQ=255)
                                                                                          cAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCCCTTGTGCGGTGCGGGGCAGTTCGGCAAGGAATTCGGGATGCCCGGAAAAGGCGATACAGCtt                                                     >  4:333693/1‑149 (MQ=255)
                                                                                                    gTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATca                                           >  8:208576/1‑149 (MQ=255)
                                                                                                     tCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATcac                                          <  7:208576/149‑1 (MQ=255)
                                                                                                     tCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATca                                           >  6:41305/1‑148 (MQ=255)
                                                                                                      cGGCAACATCACGCAGTGGCAGGTGCGTGGACACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCCGGTCAGTTCGGCAAGGAATTCGGTCTGCCCGGAAAAGGCGATACCGCTTTCGTTGATcaca                                         >  7:205023/1‑149 (MQ=255)
                                                                                                        gCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACAcc                                       <  8:241377/149‑1 (MQ=255)
                                                                                                             aTCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATcaca                                         >  6:317886/1‑142 (MQ=255)
                                                                                                             aTCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATcaca                                         <  5:317886/142‑1 (MQ=255)
                                                                                                             aTCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTtgt                                  <  6:265571/149‑1 (MQ=255)
                                                                                                                  gCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATAc                                                          <  4:333124/120‑1 (MQ=255)
                                                                                                                  gCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATAc                                                          >  3:333124/1‑120 (MQ=255)
                                                                                                                     gTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGAt                                             >  3:50639/1‑130 (MQ=255)
                                                                                                                     gTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGAt                                             <  4:50639/130‑1 (MQ=255)
                                                                                                                        gCAGGTGCGTGGTGTCGAGGGCCACGCGCAGGCCGCGGGTGGCCAGGATGATTACCACTTGTGGGGTGCGGGTCAGTTCGGCAAGGTATTCGGTATGCGCGGAAAAGGCGATACCGGTTTCGTTGATCACACCCTTGTGCACCGGGGc                        <  8:205023/148‑1 (MQ=255)
                                                                                                                        gCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAgg                                                                <  6:210459/108‑1 (MQ=255)
                                                                                                                        gCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAgg                                                                >  5:210459/1‑108 (MQ=255)
                                                                                                                         cAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCg                      <  4:60448/149‑1 (MQ=255)
                                                                                                                             tGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGa                   <  5:225022/148‑1 (MQ=255)
                                                                                                                                   gTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTACGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATccc            >  1:95556/1‑149 (MQ=255)
                                                                                                                                       ccAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCAATTGTGCGGTGCGGGTCAGTTCGTCAAGGAATTCGGTATGGCCGGAAAAGGCGATACAGATTTCGTTGATCACCCCCTTGTGCACCGGGGCCGTGATCATCCCAGc         >  2:217211/1‑148 (MQ=255)
                                                                                                                                        cAGGGCCACGCGCAGGCCGCGGGTGGCCGGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCg        >  3:3515/1‑148 (MQ=255)
                                                                                                                                        cAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCg        >  5:172969/1‑148 (MQ=255)
                                                                                                                                         aGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGtt                                                >  3:331755/1‑107 (MQ=255)
                                                                                                                                         aGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGtt                                                <  4:331755/107‑1 (MQ=255)
                                                                                                                                            gCCACCCGCGGCCTGCGCGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTg   >  2:128925/1‑149 (MQ=255)
                                                                                                                                             ccACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGc  <  4:293091/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGCCGCACACGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGC  >  NC_002947/487680‑487969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑