Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 487,829 A→G 6.5% L158P (CTG→CCG)  pdxA ← 4‑hydroxythreonine‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947487,8290AG 6.5% 177.7 / 2.6 62L158P (CTG→CCG) pdxA4‑hydroxythreonine‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (29/28);  new base G (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

GCCGCACACGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTC  >  NC_002947/487681‑487974
                                                                                                                                                    |                                                                                                                                                 
gCCGCACACGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCa                                                                                                                                                   <  1:61989/149‑1 (MQ=255)
   gCACACGAGGATACTCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGc                                                                                                                                                >  1:310591/1‑149 (MQ=255)
        cGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCgg                                                                                                                                           <  6:160083/149‑1 (MQ=255)
         gAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCggg                                                                                                                                          >  3:198661/1‑149 (MQ=255)
           ggATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTg                                                                                                                                        <  4:145603/149‑1 (MQ=255)
            gATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACCCGCGGCCTGCGCGTgg                                                                                                                                       <  4:309083/149‑1 (MQ=255)
                 gcgGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAg                                                                                                                                   >  6:144357/1‑148 (MQ=255)
                 gcgGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACCCGCGGCCTGCGCGTGGc                                                                                                                                      >  6:82856/1‑145 (MQ=255)
                 gcgGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACCCGCGGCCTGCGCGTGGc                                                                                                                                      <  5:82856/145‑1 (MQ=255)
                 gcgGGTTGGCAATGCCAAACTTGGCGCGCATGTCGGCATGCAGGATACGGTTTACCCGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCatg                                                                                                                                                 <  7:220881/134‑3 (MQ=255)
                        ggCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCAtt                                                                                                                            <  8:158868/148‑1 (MQ=255)
                        ggCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCAtt                                                                                                                            <  4:279715/148‑1 (MQ=255)
                           aaTGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCa                                                                                                                        <  3:185218/149‑1 (MQ=255)
                           aaTGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCa                                                                                                                        <  6:221685/149‑1 (MQ=255)
                            aTGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCAc                                                                                                                       <  7:301484/149‑1 (MQ=255)
                               ccAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCTGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtg                                                                                                                    <  4:13916/149‑1 (MQ=255)
                                cAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCGGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtgt                                                                                                                   >  5:115597/1‑149 (MQ=255)
                                    cTTGTCGCGCATGTCGGCATGCAGGATAGGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTgtgcgg                                                                                                               >  1:196888/1‑149 (MQ=255)
                                          gcgcATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCCTCATTGCCACTTGTGCGGTGCggg                                                                                                         >  4:96976/1‑149 (MQ=255)
                                          gcgcATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGtgcggtgcgg                                                                                                          <  7:314980/148‑1 (MQ=255)
                                          gcgcATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGtgcggtgcgg                                                                                                          <  2:196888/148‑1 (MQ=255)
                                              aTGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtgtg                                                                                                                  >  3:68616/1‑136 (MQ=255)
                                              aTGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtgtg                                                                                                                  <  4:68616/136‑1 (MQ=255)
                                                  cGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCg                                                                                                 >  5:157567/1‑149 (MQ=255)
                                                    gCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCgg                                                                                                >  3:293091/1‑148 (MQ=255)
                                                       tGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAg                                                                                            <  5:144357/149‑1 (MQ=255)
                                                         cAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGa                                                                                          <  4:198661/149‑1 (MQ=255)
                                                           ggATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATAATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAAt                                                                                        >  3:60448/1‑149 (MQ=255)
                                                            gATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACCTCACGCAGTGGCCGGTGCGTGGTCACCAGGGCCCAGCGCCGGCCGGGGGTGGCCAGGCTCATTGGCCCTGGTGGGGTGCGGGGCCGGTCGGCAAGGagt                                                                                        >  6:345289/1‑146 (MQ=255)
                                                                 gggTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTa                                                                                  >  7:241377/1‑149 (MQ=255)
                                                                    ttACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGc                                                                               >  5:265571/1‑149 (MQ=255)
                                                                        acGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCgg                                                                           <  2:310591/149‑1 (MQ=255)
                                                                        acGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCg                                                                            >  5:155246/1‑148 (MQ=255)
                                                                               aCGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACGAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGt                                                                                   <  5:4742/134‑1 (MQ=255)
                                                                               aCGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCACGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGt                                                                                   >  6:4742/1‑134 (MQ=255)
                                                                                 gcgcTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGa                                                                  <  6:155246/149‑1 (MQ=255)
                                                                                       tGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGc                                                            >  4:293809/1‑149 (MQ=255)
                                                                                         cAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCCCTTGTGCGGTGCGGGGCAGTTCGGCAAGGAATTCGGGATGCCCGGAAAAGGCGATACAGCtt                                                          >  4:333693/1‑149 (MQ=255)
                                                                                                   gTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATca                                                >  8:208576/1‑149 (MQ=255)
                                                                                                    tCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATcac                                               <  7:208576/149‑1 (MQ=255)
                                                                                                    tCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATca                                                >  6:41305/1‑148 (MQ=255)
                                                                                                     cGGCAACATCACGCAGTGGCAGGTGCGTGGACACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCCGGTCAGTTCGGCAAGGAATTCGGTCTGCCCGGAAAAGGCGATACCGCTTTCGTTGATcaca                                              >  7:205023/1‑149 (MQ=255)
                                                                                                       gCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACAcc                                            <  8:241377/149‑1 (MQ=255)
                                                                                                            aTCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATcaca                                              <  5:317886/142‑1 (MQ=255)
                                                                                                            aTCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATcaca                                              >  6:317886/1‑142 (MQ=255)
                                                                                                            aTCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTtgt                                       <  6:265571/149‑1 (MQ=255)
                                                                                                                 gCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATAc                                                               >  3:333124/1‑120 (MQ=255)
                                                                                                                 gCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATAc                                                               <  4:333124/120‑1 (MQ=255)
                                                                                                                    gTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGAt                                                  >  3:50639/1‑130 (MQ=255)
                                                                                                                    gTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGAt                                                  <  4:50639/130‑1 (MQ=255)
                                                                                                                       gCAGGTGCGTGGTGTCGAGGGCCACGCGCAGGCCGCGGGTGGCCAGGATGATTACCACTTGTGGGGTGCGGGTCAGTTCGGCAAGGTATTCGGTATGCGCGGAAAAGGCGATACCGGTTTCGTTGATCACACCCTTGTGCACCGGGGc                             <  8:205023/148‑1 (MQ=255)
                                                                                                                       gCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAgg                                                                     >  5:210459/1‑108 (MQ=255)
                                                                                                                       gCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAgg                                                                     <  6:210459/108‑1 (MQ=255)
                                                                                                                        cAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCg                           <  4:60448/149‑1 (MQ=255)
                                                                                                                            tGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGa                        <  5:225022/148‑1 (MQ=255)
                                                                                                                                  gTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTACGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATccc                 >  1:95556/1‑149 (MQ=255)
                                                                                                                                      ccAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCAATTGTGCGGTGCGGGTCAGTTCGTCAAGGAATTCGGTATGGCCGGAAAAGGCGATACAGATTTCGTTGATCACCCCCTTGTGCACCGGGGCCGTGATCATCCCAGc              >  2:217211/1‑148 (MQ=255)
                                                                                                                                       cAGGGCCACGCGCAGGCCGCGGGTGGCCGGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCg             >  3:3515/1‑148 (MQ=255)
                                                                                                                                       cAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCg             >  5:172969/1‑148 (MQ=255)
                                                                                                                                        aGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGtt                                                     <  4:331755/107‑1 (MQ=255)
                                                                                                                                        aGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGtt                                                     >  3:331755/1‑107 (MQ=255)
                                                                                                                                           gCCACCCGCGGCCTGCGCGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTg        >  2:128925/1‑149 (MQ=255)
                                                                                                                                            ccACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGc       <  4:293091/149‑1 (MQ=255)
                                                                                                                                                 cgcAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTc  >  4:77183/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCCGCACACGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTC  >  NC_002947/487681‑487974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑