Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,857,965 C→G 5.7% R172G (CGG→GGG)  PP_5134 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,857,9650CG 5.7% 225.0 / 3.8 70R172G (CGG→GGG) PP_5134hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/37);  new base G (2/2);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.12e-01

TGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAGCG  >  NC_002947/5857820‑5858110
                                                                                                                                                 |                                                                                                                                                 
tgtgATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCgg                                                                                                                                                 <  5:313403/148‑1 (MQ=255)
tgtgATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCgg                                                                                                                                                 <  2:86964/148‑1 (MQ=255)
  tgATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCtt                                                                                                                                              >  8:172236/1‑149 (MQ=255)
    aTCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTgg                                                                                                                                            <  1:246677/149‑1 (MQ=255)
    aTCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTg                                                                                                                                             <  7:100443/148‑1 (MQ=255)
    aTCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTg                                                                                                                                             <  1:64027/148‑1 (MQ=255)
     tCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTggg                                                                                                                                           >  6:172058/1‑149 (MQ=255)
       gATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGc                                                                                                                                          <  8:119812/148‑1 (MQ=255)
           cgcgcgAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCAAGCCGGGCAAGGGCTTCAc                                                                                                                                     <  4:279161/149‑1 (MQ=255)
                gAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCAtt                                                                                                                                >  5:194945/1‑149 (MQ=255)
                 aGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTg                                                                                                                               <  5:277296/149‑1 (MQ=255)
                    cGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTa                                                                                                                            <  1:112404/149‑1 (MQ=255)
                    cGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTa                                                                                                                            <  8:248094/149‑1 (MQ=255)
                     ggTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTAc                                                                                                                           <  5:126175/149‑1 (MQ=255)
                                    ccGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCAc                                                                                                                                     >  5:283487/1‑124 (MQ=255)
                                    ccGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCAc                                                                                                                                     <  6:283487/124‑1 (MQ=255)
                                    ccGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTgg                                                                                                            >  4:11028/1‑149 (MQ=255)
                                     cgcgACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGc                                                                                                           >  1:58650/1‑149 (MQ=255)
                                        gacgaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGcccc                                                                                                        >  6:10153/1‑149 (MQ=255)
                                        gacgaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGcccc                                                                                                        <  6:194945/149‑1 (MQ=255)
                                          cgaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTTGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGc                                                                                                      <  7:172236/149‑1 (MQ=255)
                                          cgaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGc                                                                                                      <  5:172058/149‑1 (MQ=255)
                                          cgaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGc                                                                                                      <  6:36837/149‑1 (MQ=255)
                                           gaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGa                                                                                                                    <  5:215938/134‑1 (MQ=255)
                                           gaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGa                                                                                                                    >  6:215938/1‑134 (MQ=255)
                                           gaACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGTCAGCGTGGCCCCGcc                                                                                                     <  8:89049/149‑1 (MQ=255)
                                                 gAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCagag                                                                                                 <  3:249099/147‑1 (MQ=255)
                                                 gAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGa                                                                                               >  1:84424/1‑149 (MQ=255)
                                                     tGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGt                                                                                           <  2:84424/149‑1 (MQ=255)
                                                       cTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCtt                                                                                                                                              <  2:104986/96‑1 (MQ=255)
                                                       cTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGa                                                                                         <  7:19187/149‑1 (MQ=255)
                                                          gCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAg                                                                                        <  2:58650/147‑1 (MQ=255)
                                                               cTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGtt                                                                                 >  1:256726/1‑149 (MQ=255)
                                                                    gCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTAc                                                                            <  2:256726/149‑1 (MQ=255)
                                                                        gCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGaaa                                                                        <  5:10153/149‑1 (MQ=255)
                                                                        gCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGaaa                                                                        <  6:316644/149‑1 (MQ=255)
                                                                          cGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGc                                                                      >  6:279426/1‑149 (MQ=255)
                                                                                 gcCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGgcgc                                                               >  1:191398/1‑149 (MQ=255)
                                                                                   cGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCgctg                                                             <  3:11028/149‑1 (MQ=255)
                                                                                         tCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGTCAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGctgctgctg                                                       >  7:205195/1‑149 (MQ=255)
                                                                                          cAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGgc                                                                 <  8:96659/138‑1 (MQ=255)
                                                                                          cAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGgc                                                                 >  7:96659/1‑138 (MQ=255)
                                                                                           aaCTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTa                                                                             >  5:275274/1‑125 (MQ=255)
                                                                                           aaCTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTa                                                                             <  6:275274/125‑1 (MQ=255)
                                                                                           aaCTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGctgctgctg                                                       <  8:270866/147‑1 (MQ=255)
                                                                                           aaCTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTgg                                                      <  7:19655/148‑1 (MQ=255)
                                                                                               tcgtcgCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCgctg                                                             >  1:153950/1‑137 (MQ=255)
                                                                                               tcgtcgCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCgctg                                                             <  2:153950/137‑1 (MQ=255)
                                                                                                  tcgCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCAGGCGCTGCTGCTGGAAAAcgcc                                              >  1:278652/1‑149 (MQ=255)
                                                                                                     ccagcagccagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAcgcccgc                                           >  7:195710/1‑149 (MQ=255)
                                                                                                             cagcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCt                                   >  3:24704/1‑149 (MQ=255)
                                                                                                               gcaCCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTg                                  >  8:44951/1‑148 (MQ=255)
                                                                                                               gcaCCGCGAGCCTGAATGGCGTGAAACCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAcgc                                               <  4:229000/135‑1 (MQ=255)
                                                                                                               gcaCCGCGAGCCTGAATGGCGTGAAACCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAcgc                                               >  3:229000/1‑135 (MQ=255)
                                                                                                                   cgcgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACg                             >  1:69308/1‑149 (MQ=255)
                                                                                                                     cgAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGc                           <  2:191398/149‑1 (MQ=255)
                                                                                                                         ccTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAACGCCTGGCGCTGCTGCTGGAAACCGCCCGCCCGGCGCTGCAACGGCTGag                       >  8:233593/1‑148 (MQ=255)
                                                                                                                          cTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATc                      <  1:223847/149‑1 (MQ=255)
                                                                                                                              aatgCGTGAAGCCCAAGCCGGGCAAGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGttt                                                                                >  7:93391/4‑87 (MQ=25)
                                                                                                                              aatgCGTGAAGCCCAAGCCGGGCAAGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGttt                                                                                <  8:93391/84‑1 (MQ=25)
                                                                                                                                tgCGTGAAGCCCAAGCCGGGCAAGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCgacgac                >  2:21716/2‑149 (MQ=255)
                                                                                                                                      aaGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACcagcag          >  5:121598/1‑149 (MQ=255)
                                                                                                                                       aGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAcgccc                                             >  7:28429/1‑113 (MQ=255)
                                                                                                                                       aGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAAcgccc                                             <  8:28429/113‑1 (MQ=255)
                                                                                                                                         cccTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATc                      <  3:257441/134‑1 (MQ=255)
                                                                                                                                         cccTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATc                      >  4:257441/1‑134 (MQ=255)
                                                                                                                                         cccTTGACCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGAGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTCCAACGGCTGATCGACGACCAGCAGGca       >  7:170711/1‑149 (MQ=255)
                                                                                                                                            ttGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAg    >  4:121703/1‑149 (MQ=255)
                                                                                                                                              gCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAg    <  7:44951/147‑1 (MQ=255)
                                                                                                                                              gCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAGCg  >  2:161431/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TGTGATCGATGCGCGCGAGCCGGTGCTGGCCAAGTACCGCGACGAACTGGAAGTGCTGGCCAACTGCGGCAAGCCGTTGCTGCCGGTGCTCAACTTCGTCGCCAGCAGCCAGCACCGCGAGCCTGAATGGCGTGAAGCCCTTGCCCGGCTTGGGCTTCACGCATTGGTACGGTTCGACAGCGTGGCCCCGCCAGAGGATGGTGAGCGCCGTTTGTACGAAAGCCTGGCGCTGCTGCTGGAAAACGCCCGCCCGGCGCTGCAACGGCTGATCGACGACCAGCAGGCACAGCG  >  NC_002947/5857820‑5858110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑