Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 487,831 G→C 6.4% G157G (GGC→GGG)  pdxA ← 4‑hydroxythreonine‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947487,8310GC 6.4% 199.0 / 2.4 62G157G (GGC→GGG) pdxA4‑hydroxythreonine‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (30/28);  new base C (2/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GCACACGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTCCAGGC  >  NC_002947/487684‑487979
                                                                                                                                                   |                                                                                                                                                    
gCACACGAGGATACTCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGc                                                                                                                                                     >  1:310591/1‑149 (MQ=255)
     cGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCgg                                                                                                                                                <  6:160083/149‑1 (MQ=255)
      gAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCggg                                                                                                                                               >  3:198661/1‑149 (MQ=255)
        ggATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTg                                                                                                                                             <  4:145603/149‑1 (MQ=255)
         gATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACCCGCGGCCTGCGCGTgg                                                                                                                                            <  4:309083/149‑1 (MQ=255)
              gcgGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAg                                                                                                                                        >  6:144357/1‑148 (MQ=255)
              gcgGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACCCGCGGCCTGCGCGTGGc                                                                                                                                           >  6:82856/1‑145 (MQ=255)
              gcgGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACCCGCGGCCTGCGCGTGGc                                                                                                                                           <  5:82856/145‑1 (MQ=255)
                     ggCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCAtt                                                                                                                                 <  8:158868/148‑1 (MQ=255)
                     ggCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCAtt                                                                                                                                 <  4:279715/148‑1 (MQ=255)
                        aaTGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCa                                                                                                                             <  3:185218/149‑1 (MQ=255)
                        aaTGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCa                                                                                                                             <  6:221685/149‑1 (MQ=255)
                         aTGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCAc                                                                                                                            <  7:301484/149‑1 (MQ=255)
                            ccAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCTGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtg                                                                                                                         <  4:13916/149‑1 (MQ=255)
                             cAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCGGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtgt                                                                                                                        >  5:115597/1‑149 (MQ=255)
                                 cTTGTCGCGCATGTCGGCATGCAGGATAGGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTgtgcgg                                                                                                                    >  1:196888/1‑149 (MQ=255)
                                       gcgcATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCCTCATTGCCACTTGTGCGGTGCggg                                                                                                              >  4:96976/1‑149 (MQ=255)
                                       gcgcATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGtgcggtgcgg                                                                                                               <  7:314980/148‑1 (MQ=255)
                                       gcgcATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGtgcggtgcgg                                                                                                               <  2:196888/148‑1 (MQ=255)
                                           aTGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtgtg                                                                                                                       >  3:68616/1‑136 (MQ=255)
                                           aTGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtgtg                                                                                                                       <  4:68616/136‑1 (MQ=255)
                                               cGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCg                                                                                                      >  5:157567/1‑149 (MQ=255)
                                                 gCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCgg                                                                                                     >  3:293091/1‑148 (MQ=255)
                                                    tGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAg                                                                                                 <  5:144357/149‑1 (MQ=255)
                                                      cAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGa                                                                                               <  4:198661/149‑1 (MQ=255)
                                                        ggATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATAATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAAt                                                                                             >  3:60448/1‑149 (MQ=255)
                                                         gATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACCTCACGCAGTGGCCGGTGCGTGGTCACCAGGGCCCAGCGCCGGCCGGGGGTGGCCAGGCTCATTGGCCCTGGTGGGGTGCGGGGCCGGTCGGCAAGGagt                                                                                             >  6:345289/1‑146 (MQ=255)
                                                              gggTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTa                                                                                       >  7:241377/1‑149 (MQ=255)
                                                                 ttACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGc                                                                                    >  5:265571/1‑149 (MQ=255)
                                                                     acGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCgg                                                                                <  2:310591/149‑1 (MQ=255)
                                                                     acGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCg                                                                                 >  5:155246/1‑148 (MQ=255)
                                                                            aCGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACGAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGt                                                                                        <  5:4742/134‑1 (MQ=255)
                                                                            aCGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCACGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGt                                                                                        >  6:4742/1‑134 (MQ=255)
                                                                              gcgcTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGa                                                                       <  6:155246/149‑1 (MQ=255)
                                                                                    tGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGc                                                                 >  4:293809/1‑149 (MQ=255)
                                                                                      cAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCCCTTGTGCGGTGCGGGGCAGTTCGGCAAGGAATTCGGGATGCCCGGAAAAGGCGATACAGCtt                                                               >  4:333693/1‑149 (MQ=255)
                                                                                                gTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATca                                                     >  8:208576/1‑149 (MQ=255)
                                                                                                 tCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATcac                                                    <  7:208576/149‑1 (MQ=255)
                                                                                                 tCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATca                                                     >  6:41305/1‑148 (MQ=255)
                                                                                                  cGGCAACATCACGCAGTGGCAGGTGCGTGGACACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCCGGTCAGTTCGGCAAGGAATTCGGTCTGCCCGGAAAAGGCGATACCGCTTTCGTTGATcaca                                                   >  7:205023/1‑149 (MQ=255)
                                                                                                    gCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACAcc                                                 <  8:241377/149‑1 (MQ=255)
                                                                                                         aTCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATcaca                                                   >  6:317886/1‑142 (MQ=255)
                                                                                                         aTCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATcaca                                                   <  5:317886/142‑1 (MQ=255)
                                                                                                         aTCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTtgt                                            <  6:265571/149‑1 (MQ=255)
                                                                                                              gCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATAc                                                                    <  4:333124/120‑1 (MQ=255)
                                                                                                              gCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATAc                                                                    >  3:333124/1‑120 (MQ=255)
                                                                                                                 gTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGAt                                                       >  3:50639/1‑130 (MQ=255)
                                                                                                                 gTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGAt                                                       <  4:50639/130‑1 (MQ=255)
                                                                                                                    gCAGGTGCGTGGTGTCGAGGGCCACGCGCAGGCCGCGGGTGGCCAGGATGATTACCACTTGTGGGGTGCGGGTCAGTTCGGCAAGGTATTCGGTATGCGCGGAAAAGGCGATACCGGTTTCGTTGATCACACCCTTGTGCACCGGGGc                                  <  8:205023/148‑1 (MQ=255)
                                                                                                                    gCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAgg                                                                          >  5:210459/1‑108 (MQ=255)
                                                                                                                    gCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAgg                                                                          <  6:210459/108‑1 (MQ=255)
                                                                                                                     cAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCg                                <  4:60448/149‑1 (MQ=255)
                                                                                                                         tGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGa                             <  5:225022/148‑1 (MQ=255)
                                                                                                                               gTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTACGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATccc                      >  1:95556/1‑149 (MQ=255)
                                                                                                                                   ccAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCAATTGTGCGGTGCGGGTCAGTTCGTCAAGGAATTCGGTATGGCCGGAAAAGGCGATACAGATTTCGTTGATCACCCCCTTGTGCACCGGGGCCGTGATCATCCCAGc                   >  2:217211/1‑148 (MQ=255)
                                                                                                                                    cAGGGCCACGCGCAGGCCGCGGGTGGCCGGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCg                  >  3:3515/1‑148 (MQ=255)
                                                                                                                                    cAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCg                  >  5:172969/1‑148 (MQ=255)
                                                                                                                                     aGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGtt                                                          >  3:331755/1‑107 (MQ=255)
                                                                                                                                     aGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGtt                                                          <  4:331755/107‑1 (MQ=255)
                                                                                                                                        gCCACCCGCGGCCTGCGCGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTg             >  2:128925/1‑149 (MQ=255)
                                                                                                                                         ccACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGc            <  4:293091/149‑1 (MQ=255)
                                                                                                                                              cgcAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTc       >  4:77183/1‑149 (MQ=255)
                                                                                                                                                   gCCGCGGGTGGCCAGCATCATTACCTCTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTCCAGGc  <  2:17304/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCACACGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTCCAGGC  >  NC_002947/487684‑487979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑