Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,905,377 C→G 9.0% A95A (GCG→GCC)  spuG ← spermidine/putrescine ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,905,3770CG 9.0% 161.6 / 5.6 55A95A (GCG→GCC) spuGspermidine/putrescine ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (22/28);  new base G (2/3);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATAGACGCGGATCAGGATCGCGGTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCTTGTCTTCGACGTAGGTGTAGATCTCGGTATACGGCGGAATTGCCACGTCAGCTTC  >  NC_002947/5905237‑5905523
                                                                                                                                            |                                                                                                                                                  
gCATAGACGCGGATCAGGATCGCGGTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCtt                                                                                                                                            >  5:116688/1‑149 (MQ=255)
  aTAGACGCGGATCAGGATCGCGGTCCAGGGTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCtt                                                                                                                                            >  2:170518/1‑147 (MQ=255)
     gACGCGGATCAGGATCGCGGTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGACACAGCAGGGTGCTGAAGATGGCGTTCTTCAGca                                                                                                                                       <  6:23184/149‑2 (MQ=255)
        gcgGATCAGGATCGCGGTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAAc                                                                                                                                    >  3:228777/1‑149 (MQ=255)
                  aTCGCGGTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGATGGCGTTCTTCAGca                                                                                                                                       <  8:117089/136‑2 (MQ=255)
                  aTCGCGGTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGATGGCGTTCTTCAGca                                                                                                                                       >  7:117089/1‑135 (MQ=255)
                       ggTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCgaga                                                                                                                     <  3:201398/149‑1 (MQ=255)
                       ggTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCgaga                                                                                                                     <  3:124068/149‑1 (MQ=255)
                        gTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCgag                                                                                                                      >  1:29322/1‑147 (MQ=255)
                        gTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCgag                                                                                                                      <  2:29322/147‑1 (MQ=255)
                        gTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGagag                                                                                                                    <  7:39117/149‑1 (MQ=255)
                        gTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGagag                                                                                                                    <  5:289526/149‑1 (MQ=255)
                         tCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGATGGCGTTCTTCAGca                                                                                                                                       <  4:338684/129‑2 (MQ=255)
                         tCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGATGGCGTTCTTCAGca                                                                                                                                       >  3:338684/1‑128 (MQ=255)
                           cAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGt                                                                                                                  <  6:116688/148‑1 (MQ=255)
                             ggTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTa                                                                                                                 >  5:28595/1‑147 (MQ=255)
                                 ggCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCt                                                                                                            >  6:325669/1‑148 (MQ=255)
                                  gCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTc                                                                                                           >  7:31824/1‑148 (MQ=255)
                                  gCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTc                                                                                                           >  6:332176/1‑148 (MQ=255)
                                   catcatGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCAt                                                                                                         >  6:160446/1‑149 (MQ=255)
                                        tGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCg                                                                                                    <  1:170518/149‑1 (MQ=255)
                                              gCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCaa                                                                                               >  2:149237/1‑148 (MQ=255)
                                                aaCAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGa                                                                                                                               <  7:196346/114‑1 (MQ=255)
                                                aaCAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGa                                                                                                                               >  8:196346/1‑114 (MQ=255)
                                                     cagGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGt                                                                                       <  6:28595/149‑1 (MQ=255)
                                                         actgTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTc                                                                                                 >  3:124010/4‑135 (MQ=255)
                                                         actgTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTc                                                                                                 <  4:124010/132‑1 (MQ=255)
                                                         aCCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGtt                                                                                   <  3:114597/149‑1 (MQ=255)
                                                               tGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAg                                                                             <  7:226556/149‑1 (MQ=255)
                                                                 ggTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGt                                                                           <  5:325669/149‑1 (MQ=255)
                                                                    ctcCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTgg                                                                                 >  3:119191/1‑140 (MQ=255)
                                                                    ctcCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTgg                                                                                 <  4:119191/140‑1 (MQ=255)
                                                                      ccTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGcc                                                                                                                                                 >  7:258477/1‑74 (MQ=255)
                                                                      ccTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGcc                                                                                                                                                 <  8:258477/74‑1 (MQ=255)
                                                                       cttcttGGCGTTGGCAATGTCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAg                                                                       <  4:228777/147‑1 (MQ=255)
                                                                       cttcttGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGa                                                                        <  8:31824/146‑1 (MQ=255)
                                                                       cttcttGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAg                                                                       <  1:149237/147‑1 (MQ=255)
                                                                          cttGGCGATGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCa                                                                              >  4:88121/1‑137 (MQ=255)
                                                                          cttGGCGATGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCa                                                                              <  3:88121/137‑1 (MQ=255)
                                                                                 ttGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGAt                                                           <  5:160446/149‑1 (MQ=255)
                                                                                      aaTGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAg                                                                 >  5:61953/1‑138 (MQ=255)
                                                                                      aaTGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAg                                                                 <  6:61953/138‑1 (MQ=255)
                                                                                           cGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCTTGTCTTc                                                  >  3:134192/1‑148 (MQ=255)
                                                                                             tAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCTTGCCTTCGAc                                               >  5:232658/1‑149 (MQ=255)
                                                                                                 ccATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCTTGTCTTCGACGTAg                                           <  4:134192/149‑1 (MQ=255)
                                                                                                 ccATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCTTGTCTTCGACGTAg                                           <  5:332176/149‑1 (MQ=255)
                                                                                                          aGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCTTGTCTTCGACGTAGGTGTAGAtc                                  >  1:49913/1‑149 (MQ=255)
                                                                                                            ccGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGTATCTTGCCTTCGACGTAGGTGTAGAtctc                                <  6:232658/149‑1 (MQ=255)
                                                                                                                    cagGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCTTGTCTTCGACGTAGGTGTAGATCTCGGTATAcg                        >  3:248856/1‑149 (MQ=255)
                                                                                                                       gCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCTTGt                                                      >  8:83868/1‑116 (MQ=255)
                                                                                                                       gCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCTTGt                                                      <  7:83868/116‑1 (MQ=255)
                                                                                                                                    cTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCTTGTCTTCGACGTAGGTGTAGATCTCGGTATACGGCGGAATTGCCACGTc        <  4:248856/149‑1 (MQ=255)
                                                                                                                                     tGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCtt                                                        <  2:164005/100‑1 (MQ=255)
                                                                                                                                     tGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCtt                                                        >  1:164005/1‑100 (MQ=255)
                                                                                                                                           aCGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGTATCTTGTCTTCGACGTAGGTGTAGATCTCGGTATACGGCGGAATTGCCACGTCAGCTTc  <  2:49913/148‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                  
GCATAGACGCGGATCAGGATCGCGGTCCAGGTTGGCATCATGATCAGCAACAGCAGGACCGTCTGGGTCTCCTTCTTGGCGTTGGCAATGGCGTAGGCCATCGGGAAGCCGATCAGCAGGCACAGCAGGGTGCTGAAGAACGCCATCTTCAGCGAACCCAGATAGGCCGAGAGGTACAGCTCATCCTCGGTCAACAGCCCGTAGTTGGCCAGGTTGAGGACCAGCTGGATCTTGTCTTCGACGTAGGTGTAGATCTCGGTATACGGCGGAATTGCCACGTCAGCTTC  >  NC_002947/5905237‑5905523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑