Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 487,837 G→C 6.5% T155T (ACC→ACG)  pdxA ← 4‑hydroxythreonine‑4‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947487,8370GC 6.5% 195.6 / 4.0 62T155T (ACC→ACG) pdxA4‑hydroxythreonine‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (28/30);  new base C (2/2);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-01

CGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTCCAGGCAGC  >  NC_002947/487689‑487982
                                                                                                                                                    |                                                                                                                                                 
cGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCgg                                                                                                                                                   <  6:160083/149‑1 (MQ=255)
 gAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCggg                                                                                                                                                  >  3:198661/1‑149 (MQ=255)
   ggATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTg                                                                                                                                                <  4:145603/149‑1 (MQ=255)
    gATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACCCGCGGCCTGCGCGTgg                                                                                                                                               <  4:309083/149‑1 (MQ=255)
         gcgGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAg                                                                                                                                           >  6:144357/1‑148 (MQ=255)
         gcgGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACCCGCGGCCTGCGCGTGGc                                                                                                                                              >  6:82856/1‑145 (MQ=255)
         gcgGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACCCGCGGCCTGCGCGTGGc                                                                                                                                              <  5:82856/145‑1 (MQ=255)
                ggCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCAtt                                                                                                                                    <  8:158868/148‑1 (MQ=255)
                ggCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCAtt                                                                                                                                    <  4:279715/148‑1 (MQ=255)
                   aaTGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCa                                                                                                                                <  6:221685/149‑1 (MQ=255)
                   aaTGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCa                                                                                                                                <  3:185218/149‑1 (MQ=255)
                    aTGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCAc                                                                                                                               <  7:301484/149‑1 (MQ=255)
                       ccAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCTGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtg                                                                                                                            <  4:13916/149‑1 (MQ=255)
                        cAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCGGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtgt                                                                                                                           >  5:115597/1‑149 (MQ=255)
                            cTTGTCGCGCATGTCGGCATGCAGGATAGGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTgtgcgg                                                                                                                       >  1:196888/1‑149 (MQ=255)
                                  gcgcATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCCTCATTGCCACTTGTGCGGTGCggg                                                                                                                 >  4:96976/1‑149 (MQ=255)
                                  gcgcATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGtgcggtgcgg                                                                                                                  <  7:314980/148‑1 (MQ=255)
                                  gcgcATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGtgcggtgcgg                                                                                                                  <  2:196888/148‑1 (MQ=255)
                                      aTGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtgtg                                                                                                                          >  3:68616/1‑136 (MQ=255)
                                      aTGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTtgtg                                                                                                                          <  4:68616/136‑1 (MQ=255)
                                          cGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCg                                                                                                         >  5:157567/1‑149 (MQ=255)
                                            gCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCgg                                                                                                        >  3:293091/1‑148 (MQ=255)
                                               tGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAg                                                                                                    <  5:144357/149‑1 (MQ=255)
                                                 cAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGa                                                                                                  <  4:198661/149‑1 (MQ=255)
                                                   ggATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATAATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAAt                                                                                                >  3:60448/1‑149 (MQ=255)
                                                    gATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACCTCACGCAGTGGCCGGTGCGTGGTCACCAGGGCCCAGCGCCGGCCGGGGGTGGCCAGGCTCATTGGCCCTGGTGGGGTGCGGGGCCGGTCGGCAAGGagt                                                                                                >  6:345289/1‑146 (MQ=255)
                                                         gggTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTa                                                                                          >  7:241377/1‑149 (MQ=255)
                                                            ttACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGc                                                                                       >  5:265571/1‑149 (MQ=255)
                                                                acGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCgg                                                                                   <  2:310591/149‑1 (MQ=255)
                                                                acGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCg                                                                                    >  5:155246/1‑148 (MQ=255)
                                                                       aCGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACGAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGt                                                                                           <  5:4742/134‑1 (MQ=255)
                                                                       aCGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCACGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGt                                                                                           >  6:4742/1‑134 (MQ=255)
                                                                         gcgcTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGa                                                                          <  6:155246/149‑1 (MQ=255)
                                                                               tGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGc                                                                    >  4:293809/1‑149 (MQ=255)
                                                                                 cAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCCCTTGTGCGGTGCGGGGCAGTTCGGCAAGGAATTCGGGATGCCCGGAAAAGGCGATACAGCtt                                                                  >  4:333693/1‑149 (MQ=255)
                                                                                           gTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATca                                                        >  8:208576/1‑149 (MQ=255)
                                                                                            tCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATcac                                                       <  7:208576/149‑1 (MQ=255)
                                                                                            tCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATca                                                        >  6:41305/1‑148 (MQ=255)
                                                                                             cGGCAACATCACGCAGTGGCAGGTGCGTGGACACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCCGGTCAGTTCGGCAAGGAATTCGGTCTGCCCGGAAAAGGCGATACCGCTTTCGTTGATcaca                                                      >  7:205023/1‑149 (MQ=255)
                                                                                               gCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACAcc                                                    <  8:241377/149‑1 (MQ=255)
                                                                                                    aTCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATcaca                                                      <  5:317886/142‑1 (MQ=255)
                                                                                                    aTCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATcaca                                                      >  6:317886/1‑142 (MQ=255)
                                                                                                    aTCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTtgt                                               <  6:265571/149‑1 (MQ=255)
                                                                                                         gCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATAc                                                                       <  4:333124/120‑1 (MQ=255)
                                                                                                         gCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATAc                                                                       >  3:333124/1‑120 (MQ=255)
                                                                                                            gTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGAt                                                          >  3:50639/1‑130 (MQ=255)
                                                                                                            gTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGAt                                                          <  4:50639/130‑1 (MQ=255)
                                                                                                               gCAGGTGCGTGGTGTCGAGGGCCACGCGCAGGCCGCGGGTGGCCAGGATGATTACCACTTGTGGGGTGCGGGTCAGTTCGGCAAGGTATTCGGTATGCGCGGAAAAGGCGATACCGGTTTCGTTGATCACACCCTTGTGCACCGGGGc                                     <  8:205023/148‑1 (MQ=255)
                                                                                                               gCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAgg                                                                             >  5:210459/1‑108 (MQ=255)
                                                                                                               gCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAgg                                                                             <  6:210459/108‑1 (MQ=255)
                                                                                                                cAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCg                                   <  4:60448/149‑1 (MQ=255)
                                                                                                                    tGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGa                                <  5:225022/148‑1 (MQ=255)
                                                                                                                          gTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTACGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATccc                         >  1:95556/1‑149 (MQ=255)
                                                                                                                              ccAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCAATTGTGCGGTGCGGGTCAGTTCGTCAAGGAATTCGGTATGGCCGGAAAAGGCGATACAGATTTCGTTGATCACCCCCTTGTGCACCGGGGCCGTGATCATCCCAGc                      >  2:217211/1‑148 (MQ=255)
                                                                                                                               cAGGGCCACGCGCAGGCCGCGGGTGGCCGGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCg                     >  3:3515/1‑148 (MQ=255)
                                                                                                                               cAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCg                     >  5:172969/1‑148 (MQ=255)
                                                                                                                                aGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGtt                                                             >  3:331755/1‑107 (MQ=255)
                                                                                                                                aGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGtt                                                             <  4:331755/107‑1 (MQ=255)
                                                                                                                                   gCCACCCGCGGCCTGCGCGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTg                >  2:128925/1‑149 (MQ=255)
                                                                                                                                    ccACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGc               <  4:293091/149‑1 (MQ=255)
                                                                                                                                         cgcAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTc          >  4:77183/1‑149 (MQ=255)
                                                                                                                                              gCCGCGGGTGGCCAGCATCATTACCTCTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTCCAGGc     <  2:17304/149‑1 (MQ=255)
                                                                                                                                                 gcgGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTCCAGGCAGc  <  6:157567/149‑1 (MQ=255)
                                                                                                                                                 gcgGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTCCAGGCAGc  <  1:104783/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CGAGGATACGCGGGTTGGCAATGCCAAACTTGTCGCGCATGTCGGCATGCAGGATACGGGTTACACGTTCGACGCGCTCTGCAGTGATAGCGTCGGCAACATCACGCAGTGGCAGGTGCGTGGTCACCAGGGCCACGCGCAGGCCGCGGGTGGCCAGCATCATTACCACTTGTGCGGTGCGGGTCAGTTCGGCAAGGAATTCGGTATGCCCGGAAAAGGCGATACCGCTTTCGTTGATCACACCCTTGTGCACCGGGGCCGTGATCATCCCGGCGAAGTGCCCGTCCAGGCAGC  >  NC_002947/487689‑487982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑