Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,938,664 T→G 11.5% S409A (TCG→GCG)  rbbA → ribosome‑associated ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,938,6640TG11.5% 137.2 / 10.0 52S409A (TCG→GCG) rbbAribosome‑associated ATPase
Reads supporting (aligned to +/- strand):  ref base T (22/24);  new base G (2/4);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

AACCTGGCCCTGCATGCCCGCCTGTTCGACTTGCCCAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCCGCGAGCAGGGCGTGACCATTTTCCTCTCCACC  >  NC_002947/5938520‑5938810
                                                                                                                                                 |                                                                                                                                                  
aaCCTGGCCCTGCATGCCCGCCTGTTCGACTTGCCCAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCt                                                                                                                                                >  8:10090/1‑149 (MQ=255)
  ccTGGCCCTGCATGCCCGCCTGTTCGACTTGCCCAAAGCCGAGAGCGCCCAGCGGCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTg                                                                                                                                               >  1:262982/1‑149 (MQ=255)
   cTGGCCCTGCATGCCCGCCTGTTCGACTTGCCCAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGACAGGCGCTGGc                                                                                                                                             <  3:135981/149‑1 (MQ=255)
      gCCCTGCATGCCCGCCTGTTCGACTTGCCCAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTCTCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGACAGGCGCTGGcg                                                                                                                                            <  5:246304/147‑2 (MQ=255)
        ccTGCATGCCCGCCTGTTCGACTTGCCCAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTgg                                                                                                                                        <  7:63740/149‑1 (MQ=255)
               gCCCGCCTGTTCGACTTGCCCAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCt                                                                                                                                 >  5:338299/1‑149 (MQ=255)
                           gACTTGCCCAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGTAGTGGCGGTTCt                                                                                                                                 >  6:181307/1‑137 (MQ=255)
                           gACTTGCCCAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGTAGTGGCGGTTCt                                                                                                                                 <  5:181307/137‑1 (MQ=255)
                            aCTTGCCCAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGaa                                                                                                                    <  2:156025/149‑1 (MQ=255)
                            aCTTGCCCAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGaa                                                                                                                    <  7:52694/149‑1 (MQ=255)
                                 cccAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCtt                                                                                                               >  1:97708/1‑149 (MQ=255)
                                 cccAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCt                                                                                                                <  5:122618/148‑1 (MQ=255)
                                   cAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGa                                                                                                             <  3:69639/149‑1 (MQ=255)
                                          gagaGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTcga                                                                                                      <  7:10090/149‑1 (MQ=255)
                                          gagaGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTcga                                                                                                      >  3:64558/1‑149 (MQ=255)
                                                 cccAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTgg                                                                                                                                              <  2:945/102‑1 (MQ=255)
                                                          aTCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTc                                                                                                        <  3:193781/132‑1 (MQ=255)
                                                          aTCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTc                                                                                                        >  4:193781/1‑132 (MQ=255)
                                                          aTCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACAAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCgg                                                                                       >  8:244258/1‑149 (MQ=255)
                                                           tCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGACGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGc                                                                                      <  8:318011/149‑1 (MQ=255)
                                                              aTGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTc                                                                                   >  5:100793/1‑149 (MQ=255)
                                                                gAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCgg                                                                                                                      <  8:267933/112‑1 (MQ=255)
                                                                gAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCgg                                                                                                                      >  7:267933/1‑112 (MQ=255)
                                                                 aGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGAc                                                                                <  2:262982/149‑1 (MQ=255)
                                                                      aTCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCccgc                                                                           >  3:166674/1‑149 (MQ=255)
                                                                             gcTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGccgcccgc                                                                    <  2:280346/149‑1 (MQ=255)
                                                                              cTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTcc                                                                                         <  3:266913/127‑1 (MQ=255)
                                                                              cTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTCCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTcc                                                                                         >  4:266913/1‑127 (MQ=255)
                                                                               ttCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGAAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGcga                                                                  >  4:285909/1‑149 (MQ=255)
                                                                                 cGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGcga                                                                  <  7:244258/147‑1 (MQ=255)
                                                                                   aCCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCgacgac                                                              >  6:210309/1‑149 (MQ=255)
                                                                                                  ccGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCctgctg                                               >  5:28709/1‑149 (MQ=255)
                                                                                                  ccGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCctgctg                                               >  7:280425/1‑149 (MQ=255)
                                                                                                  ccGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCctgctg                                               <  5:210309/149‑1 (MQ=255)
                                                                                                   cGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTgg                                              <  4:64558/149‑1 (MQ=255)
                                                                                                    gACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTgg                                              >  1:233491/1‑148 (MQ=255)
                                                                                                     aCCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTgg                                              >  3:311540/1‑147 (MQ=255)
                                                                                                         aCCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGaa                                                                                                  >  6:295720/1‑91 (MQ=255)
                                                                                                         aCCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGaa                                                                                                  <  5:295720/91‑1 (MQ=255)
                                                                                                         aCCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAAc                                        <  6:100793/149‑1 (MQ=255)
                                                                                                         aCCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAAc                                        >  5:97490/1‑149 (MQ=255)
                                                                                                         aCCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAAc                                        <  3:215908/149‑1 (MQ=255)
                                                                                                                     ccTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCCGCGAGc                            >  2:342931/1‑149 (MQ=255)
                                                                                                                           ccTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCCGCGAGCAGGGCg                      <  2:233491/149‑1 (MQ=255)
                                                                                                                           ccTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCCGCGAGCAGGGCg                      <  8:280425/149‑1 (MQ=255)
                                                                                                                            cTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCCGCGAGCAGGGCGt                     >  1:14466/1‑149 (MQ=255)
                                                                                                                                       ccAGCGACAGGCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCcgc                                <  3:117102/127‑1 (MQ=255)
                                                                                                                                       ccAGCGACAGGCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCcgc                                >  4:117102/1‑127 (MQ=255)
                                                                                                                                        cAGCGACAGGCGCTGGCGGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCCGCGAGCAGGGCGTGa                   >  7:135983/1‑139 (MQ=255)
                                                                                                                                        cAGCGACAGGCGCTGGCGGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCCGCGAGCAGGGCGTGa                   <  8:135983/139‑1 (MQ=255)
                                                                                                                                           cgcCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCCGCGAGCAGGGCGTGACCATTTTCCtctc      <  6:28709/149‑1 (MQ=255)
                                                                                                                                             ccTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCCGCGAGCAGGGCGTGACCATTTTCCTCTcca    <  2:14466/149‑1 (MQ=255)
                                                                                                                                                 tCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCCGCGAGCAGGGCGTGACCATTTTCCTCTccacc  <  6:97490/147‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AACCTGGCCCTGCATGCCCGCCTGTTCGACTTGCCCAAAGCCGAGAGCGCCCAGC‑GCATCGATGAGCTGATCGAGCGCTTCGACCTCGCCGCTATCGCCGACCAACCGTCTGGCGCCCTGCCCCTTGGTTTGCGCCAGCGCCTGTCGCTGGCAGTGGCGGTTCTGCATCGCCCGGAAGTCTTGATCCTCGACGAACCGACCTCCGGCGTCGACCCCGCCGCCCGCGACGACTTCTGGCGCCTGCTGGTAGAACTGTCCCGCGAGCAGGGCGTGACCATTTTCCTCTCCACC  >  NC_002947/5938520‑5938810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑