Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,948,140 C→G 100% A112P (GCC→CCC)  rho ← transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,948,1400CG100.0% 178.0 / NA 52A112P (GCC→CCC) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (26/26);  total (26/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

CCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCA  >  NC_002947/5948008‑5948280
                                                                                                                                    |                                                                                                                                            
ccTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCt                                                                                                                                   >  7:309324/1‑144 (MQ=255)
ccTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCt                                                                                                                                   <  8:309324/144‑1 (MQ=255)
    ggTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCccttcct                                                                                                                          >  7:258266/1‑149 (MQ=255)
        gAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCgg                                                                                                                      >  2:39533/1‑149 (MQ=255)
          gCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTg                                                                                                                    <  6:287817/149‑1 (MQ=255)
            cGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCccttcct                                                                                                                          >  1:159956/1‑141 (MQ=255)
            cGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCccttcct                                                                                                                          <  2:159956/141‑1 (MQ=255)
                     ccTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTg                                                                                                         <  7:19456/149‑1 (MQ=255)
                          aTCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCcgac                                                                                                    >  3:279683/1‑149 (MQ=255)
                             ttCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGAt                                                                                                 >  1:157972/1‑149 (MQ=255)
                               cAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATGTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTAGCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATg                                                                                                <  8:258266/148‑1 (MQ=255)
                                  gcgcTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCGCCCCGTCCTTCGGTGGGCGGATCTTGCCGACGAGGgtgg                                                                                            >  8:109805/1‑148 (MQ=255)
                                   cgcTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTc                                                                                           <  1:39533/149‑1 (MQ=255)
                                                      gcgTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGaaa                                                                        <  7:179625/149‑1 (MQ=255)
                                                         tCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCtt                                                                                                          <  4:222936/112‑1 (MQ=255)
                                                         tCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCtt                                                                                                          >  3:222936/1‑112 (MQ=255)
                                                           aGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAAcggc                                                                    <  5:143099/148‑1 (MQ=255)
                                                            ggTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGATGTAACGATCCCATTCCTTCGGTGGGCGGATCTTGCCGCCGAGGGTGTCGCCGTTACGCAGGTTGAACcggcg                                                                   >  6:17987/1‑148 (MQ=255)
                                                                   gAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATctggc                                                           >  4:288612/1‑149 (MQ=255)
                                                                      cAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATctggctgg                                                        >  7:52917/1‑149 (MQ=255)
                                                                      cAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATctggctgg                                                        <  3:232386/149‑1 (MQ=255)
                                                                       aGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTggg                                                       <  8:52917/149‑1 (MQ=255)
                                                                         gATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGc                                                      >  8:88934/1‑148 (MQ=255)
                                                                         gATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGc                                                      >  7:55608/1‑148 (MQ=255)
                                                                            cTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGACGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATg                                                                                                <  5:193278/103‑1 (MQ=255)
                                                                            cTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATg                                                                                                >  6:193278/1‑103 (MQ=255)
                                                                               gTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCgaga                                                  <  2:157972/146‑1 (MQ=255)
                                                                               gTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCga                                                    <  8:102138/144‑1 (MQ=255)
                                                                                   ttcGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGa                                            >  4:85486/1‑148 (MQ=255)
                                                                                     cgcgTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGa                                                                 >  3:204013/1‑125 (MQ=255)
                                                                                     cgcgTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGa                                                                 <  4:204013/125‑1 (MQ=255)
                                                                                     cgcgTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGata                                          <  3:288612/148‑1 (MQ=255)
                                                                                     cgcgTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGat                                           <  6:157076/147‑1 (MQ=255)
                                                                                      gcgTTTTACGGGCGGGCGAACTTGATGGTATCGTCCGTCACCATGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATc                                        <  5:17987/149‑1 (MQ=255)
                                                                                            tCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACCATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTg                                   <  4:189336/148‑1 (MQ=255)
                                                                                                     tCGAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAg                         <  7:88934/149‑1 (MQ=255)
                                                                                                       gAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACAt                                               <  3:274009/125‑1 (MQ=255)
                                                                                                       gAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACAt                                               >  4:274009/1‑125 (MQ=255)
                                                                                                       gAAGTTGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAgg                        >  7:212742/1‑148 (MQ=255)
                                                                                                        aaGTTGATGGTAGCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTa                      <  8:55608/149‑1 (MQ=255)
                                                                                                           ttGATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGa                   <  4:279683/149‑1 (MQ=255)
                                                                                                             gATGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACg                 >  5:25526/1‑149 (MQ=255)
                                                                                                              aTGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACg                 >  7:219798/1‑148 (MQ=255)
                                                                                                              aTGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACg                 >  5:188771/1‑148 (MQ=255)
                                                                                                               tGGTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCa               <  7:109805/149‑1 (MQ=255)
                                                                                                                ggTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCAt              >  7:202462/1‑149 (MQ=255)
                                                                                                                ggTATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGAGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCAt              >  5:183833/1‑149 (MQ=255)
                                                                                                                  tATCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTACGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTCGCTGGGCGAGACATAGATATCGACTGGGCCTGCCAGGTAGGACGCAt              >  5:265622/1‑147 (MQ=255)
                                                                                                                   aTCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGt                                                                                     <  8:141239/75‑1 (MQ=255)
                                                                                                                   aTCGACCTTCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGt                                                                                     >  7:141239/1‑75 (MQ=255)
                                                                                                                          ttCAGCAGGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACg    >  6:42569/1‑149 (MQ=255)
                                                                                                                             agcagGGGGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCa  <  8:212742/148‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                            
CCTCGGTGGAGCCGTTACCGGCCTCCATCTTCAGGCGCTTGTTCGGGAACAGCGGCGTCAGGTTTTCGAACAGGATCTTGTTCTTCGCGTTTTCCGGGCGGTCGAAGTTGATGGTATCGACCTTCAGCAGGGCGAAGTAACGCTCCCCTTCCTTCGGTGGGCGGATCTTGCCGACGATGGTGTCGCCGGTACGCAGGTTGAAACGGCGGATCTGGCTGGGCGAGACATAGATATCGTCTGGGCCGGCCAGGTAGGACGCATCAGCCGAACGCA  >  NC_002947/5948008‑5948280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑