Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	499,203	A→G	100%	intergenic (+47/‑249)	uhpA → / → PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	499,203	0	A	G	100.0%	130.7 / NA	39	intergenic (+47/‑249)	uhpA/PP_0411	two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/22);  total (17/22)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGG‑CATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAAC  >  NC_002947/499057‑499344                                                                                                                                                    |                                                                                                                                               cAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCggg                                                                                                                                              <  6:128542/149‑1 (MQ=255)  aaGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGc                                                                                                                                             <  2:50429/149‑1 (MQ=255)            gCACCCTCAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgcg                                                                                                                                    >  4:205644/1‑148 (MQ=255)                ccACAAGGCACGCCTGATGCAGAAGTTGAACGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACgg                                                                                                                               <  2:279515/149‑1 (MQ=255)                ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACgg                                                                                                                               >  4:105474/1‑149 (MQ=255)                ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACgg                                                                                                                               >  6:54758/1‑149 (MQ=255)                ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑ACCGAAACTTGCCCAAGACTATGTGGGAGCGGGCATTCCCGCCAACgg                                                                                                                               >  8:304108/1‑149 (MQ=255)                   caAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcc                                                                                                                            >  7:178238/1‑149 (MQ=255)                    aaGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcct                                                                                                                           <  6:273016/149‑1 (MQ=255)                    aaGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcct                                                                                                                           <  4:233114/149‑1 (MQ=255)                    aaGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcct                                                                                                                           >  5:55299/1‑149 (MQ=255)                         aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGggcctggcct                                                                                                                      >  2:323509/1‑149 (MQ=255)                          cGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAGAggccggctcag                                                                                                                     <  3:73249/149‑8 (MQ=39)                            ccTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCctgct                                                                                                                   <  5:54758/149‑1 (MQ=255)                                aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGAtc                                                                                                               <  7:307494/149‑1 (MQ=255)                                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTTATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGAtctc                                                                                                             <  8:178238/148‑1 (MQ=255)                                       aGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATgg                                                                                                        <  3:11156/149‑1 (MQ=255)                                           gAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGCTCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTg                                                                                                     <  8:297519/148‑1 (MQ=255)                                            aaaGTTAACTCTCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGa                                                                                                    <  7:304108/148‑1 (MQ=255)                                                    cTCGCTGGCGGAGCTGGTGAAGTACGCCATGGACCACAAGCTGATCTGATTGCGACATACGGGTGTCCCGAAACTTGCCGAAGCCTATGTGGCAGCGGGCATGCCCGCGAAAGGGCCTGGCCAGCTGAACACTATGGCTGAGCCGGcc                                                                                             >  1:275623/1‑148 (MQ=255)                                                           gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                    <  3:115147/149‑1 (MQ=255)                                                           gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                    <  3:105474/149‑1 (MQ=255)                                                           gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                    <  6:55299/149‑1 (MQ=255)                                                           gCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                    <  1:323509/149‑1 (MQ=255)                                                                 cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGCGCCGGCCTCTTCGCGGGCATGc                                                                              >  2:236086/1‑149 (MQ=255)                                                                               cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAAGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCGCTAACCGGGCATGCCCGCTGCcacacgg                                                                >  5:268959/1‑146 (MQ=255)                                                                                aTGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                    <  8:324973/128‑1 (MQ=255)                                                                                aTGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg                                                                                    >  7:324973/1‑128 (MQ=255)                                                                                aTGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGc                                                                              <  1:299102/134‑1 (MQ=255)                                                                                aTGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGc                                                                              >  2:299102/1‑134 (MQ=255)                                                                                             gcagttcatTGCCACCTCCGGGGG‑CCCGAAAGTGGCCCACGCCTATGGGGGCGCGGGCATGCCCGCGAAGGGGCCTGGCCTGCTGATCTCTATGGCTGCGCCGGCCTATTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTc                                                  <  6:268959/142‑1 (MQ=255)                                                                                               atctgatTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAAGCCCGCGAACGGGCCGGGCCGGCTGTTATCTATGGGTGAGCCGGCCTCTTAGCGGGCATGCCCGCGCCCACAGGTTTAGTTGTGGGCTCg                                                 >  8:60976/1‑148 (MQ=255)                                                                                                    atTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAgg                                            <  1:236086/148‑1 (MQ=255)                                                                                                                gggTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTg                                                          >  5:60527/1‑122 (MQ=255)                                                                                                                gggTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTg                                                          <  6:60527/122‑1 (MQ=255)                                                                                                                      cccGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAAGCCCCCCCAACGGCCATGCCAGCGGAACTATAATGCTGAGCCCGCCACGTAGCCGGCAATCCCCCACCCCCAAGTTTAGTGGTTTGCGAGCCAGGGCCATACCAA‑CCCGCCag                         >  2:11082/1‑148 (MQ=255)                                                                                                                        cGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAATCCCGCGGACGGGCCTGGGCGGCTGATCTCGATGGCTGGGCCGGCCGCATAGCGGGCATGCCCGCGCCCCCACGTTTGGGGGTTGGCGCGCAAGGG‑GATTGCAAAACCGccctac                       >  6:307709/1‑145 (MQ=255)                                                                                                                                   gAAGCCTATGTGGGAGCGGGCATGCCCGCGCACGGGGCTGGCCTGCTGATCTGTATGGCTGAGCCGGCCTCTTAGCGGGCATGCCCGCTCCCACAGGTTTAGTGGTGGGCTGGCAAGGG‑GATTGCAATACCCCCCTCCATGTAGGGCgc            >  6:321562/1‑148 (MQ=37)                                                                                                                                             tgGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGG‑CATTCCAATCCCGCCATCCTTGTAGGGATCGCCCTacaa   <  5:162745/148‑1 (MQ=39)                                                                                                                                             tgGGAGCGGGAAGGCCCGCGAAGGGGCCTGGCCTGGTGATCTCTATGGCGGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGG‑CATTCCAATCCCGCCATCCTTGTAGGGATCGCCCTacaag  <  7:100095/149‑2 (MQ=38)                                                                                                                                                    |                                                                                                                                               CAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTG‑CCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGG‑CATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAAC  >  NC_002947/499057‑499344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑