Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,970,527 C→T 5.9% R256H (CGC→CAC)  PP_5236 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,970,5270CT 5.9% 255.8 / 3.9 85R256H (CGC→CAC) PP_5236hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (41/39);  new base T (3/2);  total (44/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01

GGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTGGC  >  NC_002947/5970385‑5970670
                                                                                                                                               |                                                                                                                                                
ggCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCgggg                                                                                                                                              >  6:46762/1‑147 (MQ=255)
 gCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGgcag                                                                                                                                           <  6:112211/149‑1 (MQ=255)
  ccTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGgcagc                                                                                                                                          <  6:274496/149‑1 (MQ=255)
   cTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGAGAGCCATCCAGCGCAGC‑GAATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGgcag                                                                                                                                           >  6:149305/1‑147 (MQ=255)
      cACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCTGCCCCGCTGGGGcggcag                                                                                                                                        <  3:72070/147‑1 (MQ=255)
        ccTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATGCAGCGCAGC‑GCATTTCGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTac                                                                                                                                    <  2:240606/149‑1 (MQ=255)
            gTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTacac                                                                                                                                  <  5:46762/147‑1 (MQ=255)
             tCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAg                                                                                                                                >  5:105385/1‑148 (MQ=255)
                gCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCgg                                                                                                                                                <  5:351579/129‑1 (MQ=255)
                gCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCgg                                                                                                                                                >  6:351579/1‑129 (MQ=255)
                 ccTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAgg                                                                                                                           >  5:184800/1‑149 (MQ=255)
                 ccTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATCGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCg                                                                                                                                                 >  8:158457/1‑127 (MQ=255)
                 ccTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATCGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCg                                                                                                                                                 <  7:158457/127‑1 (MQ=255)
                       gCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCt                                                                                                                       <  8:40070/147‑1 (MQ=255)
                       gCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCt                                                                                                                       >  7:40070/1‑147 (MQ=255)
                       gCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTg                                                                                                                      >  8:119599/1‑148 (MQ=255)
                         aGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGAt                                                                                                                   >  6:3089/1‑149 (MQ=255)
                              cAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCtt                                                                                                              >  5:208127/1‑149 (MQ=255)
                                gCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTc                                                                                                             >  1:193620/1‑148 (MQ=255)
                                gCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTc                                                                                                             >  3:15082/1‑148 (MQ=255)
                                gCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTc                                                                                                             >  3:322204/1‑148 (MQ=255)
                                 ccGGCCTTCGACAGCCATCCAGCGCAGCGGCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTc                                                                                                             >  1:195098/1‑148 (MQ=255)
                                 ccGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCg                                                                                                            >  7:101547/1‑148 (MQ=255)
                                  cGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGaa                                                                                                          <  2:103486/149‑1 (MQ=255)
                                    gCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAagc                                                                                                        <  6:324628/149‑1 (MQ=255)
                                       ttCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCt                                                                                                                       >  7:15030/1‑131 (MQ=255)
                                       ttCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCt                                                                                                                       <  8:15030/131‑1 (MQ=255)
                                       ttCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAagc‑agc                                                                                                    >  4:60739/1‑149 (MQ=255)
                                          gACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCgg                                                                                                 >  3:187798/1‑149 (MQ=255)
                                             aGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGgggc                                                                                              >  8:314055/1‑149 (MQ=255)
                                             aGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGgggc                                                                                              >  1:296246/1‑149 (MQ=255)
                                                cATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGTGGGGCAGCAGGTACACAGCAAGGGCCTGGAt                                                                                                                   >  3:272645/1‑126 (MQ=255)
                                                cATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGTGGGGCAGCAGGTACACAGCAAGGGCCTGGAt                                                                                                                   <  4:272645/126‑1 (MQ=255)
                                                 aTCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGgggcgggc                                                                                          <  7:22257/149‑1 (MQ=255)
                                                    cagcgcagc‑gCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGa                                                                                        <  4:15082/148‑1 (MQ=255)
                                                     agcgcagc‑gcATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATc                                                                                      <  8:101547/149‑1 (MQ=255)
                                                         cagc‑gcaTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAgcg                                                                                  <  2:296246/149‑1 (MQ=255)
                                                           gc‑gcaTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCa                                                                                <  4:322204/149‑1 (MQ=255)
                                                              gcaTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATg                                                                              <  7:226536/149‑1 (MQ=255)
                                                               caTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATgg                                                                             <  5:149854/149‑1 (MQ=255)
                                                                aTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGAt                                                                                       >  8:151195/1‑138 (MQ=255)
                                                                aTTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGAt                                                                                       <  7:151195/138‑1 (MQ=255)
                                                                     gCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAg                                                                                                                            >  4:313969/1‑97 (MQ=255)
                                                                     gCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAg                                                                                                                            <  3:313969/97‑1 (MQ=255)
                                                                     gCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGc                                                                       >  8:281731/1‑149 (MQ=255)
                                                                       aaaCGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGgcg                                                                           >  5:331954/1‑143 (MQ=255)
                                                                       aaaCGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGgcg                                                                           <  6:331954/143‑1 (MQ=255)
                                                                        aaCGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAg                                                                    <  6:105385/149‑1 (MQ=255)
                                                                        aaCGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAg                                                                    <  6:184800/149‑1 (MQ=255)
                                                                                tCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGc                                                             >  2:280371/1‑148 (MQ=255)
                                                                                  ccAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGa                                                           <  8:71576/148‑1 (MQ=255)
                                                                                  ccAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGa                                                           <  2:193620/148‑1 (MQ=255)
                                                                                  ccAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAg                                                          >  6:74365/1‑149 (MQ=255)
                                                                                    aGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGgggcggg                                                                                           <  4:46015/114‑1 (MQ=255)
                                                                                    aGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGgggcggg                                                                                           >  3:46015/1‑114 (MQ=255)
                                                                                    aGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAggg                                                        <  5:74365/149‑1 (MQ=255)
                                                                                          cgcACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTc                                                   <  2:195098/148‑1 (MQ=255)
                                                                                           gcACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGt                                                 >  4:157389/1‑149 (MQ=255)
                                                                                              caGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGtttt                                              <  7:314055/149‑1 (MQ=255)
                                                                                                   ggCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATgg                                         <  7:119599/149‑1 (MQ=255)
                                                                                                     ccATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTc                                       <  7:281731/149‑1 (MQ=255)
                                                                                                       aTTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGa                                     >  1:313811/1‑149 (MQ=255)
                                                                                                             cAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCt                               >  6:332108/1‑149 (MQ=255)
                                                                                                               aCAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTg                             >  4:117675/1‑149 (MQ=255)
                                                                                                                   cTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCa                         <  2:313811/149‑1 (MQ=255)
                                                                                                                    tCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGcc                           >  3:3775/1‑146 (MQ=255)
                                                                                                                      gAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGa                       <  3:60739/148‑1 (MQ=255)
                                                                                                                      gAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCCCATGccacac                       >  2:161956/1‑144 (MQ=255)
                                                                                                                        aCGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAg                    <  1:136116/149‑1 (MQ=255)
                                                                                                                           cATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAgcgc                 <  4:3775/149‑1 (MQ=255)
                                                                                                                            aTCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGCGACCCGGGGCGGGCGATCAGCGCATGGCGCGGCCAGTAATAGCGCGGGGCGGCGTTTTCATGGTCGCCCCGCTTGCCCAGATAgcgc                 >  1:103931/1‑149 (MQ=255)
                                                                                                                            aTCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGcgcg                >  2:38516/1‑149 (MQ=255)
                                                                                                                              cAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCgg               >  3:158984/1‑148 (MQ=255)
                                                                                                                               aaGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCgg               >  1:309759/1‑147 (MQ=255)
                                                                                                                                aGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAg            >  2:192017/1‑149 (MQ=255)
                                                                                                                                   ctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGa         >  3:213938/1‑149 (MQ=255)
                                                                                                                                   ctgctgCCCCGCTGGGGCGGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGa         >  3:73731/1‑149 (MQ=255)
                                                                                                                                   cTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGa         <  6:208127/149‑1 (MQ=255)
                                                                                                                                     gccgccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACt       >  5:184341/1‑149 (MQ=255)
                                                                                                                                      ccgccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGAt                      <  2:3759/133‑1 (MQ=255)
                                                                                                                                      ccgccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGAt                      >  1:3759/1‑133 (MQ=255)
                                                                                                                                        gccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTc                                       >  2:319442/1‑114 (MQ=255)
                                                                                                                                        gccCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTc                                       <  1:319442/114‑1 (MQ=255)
                                                                                                                                         ccccAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTgg   <  5:149305/149‑1 (MQ=255)
                                                                                                                                          cccAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTGGc  <  3:117675/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
GGCCTGCACCTTGTCAGCCTGTGGCAGCTTCAGCCGGCCTTCGACAGCCATCCAGCGCAGC‑GCATTACGCAAACGCTCGTCCCAGGTTGCGCACAGCGGGCCATTGACCAACAGCTCGAACGCATCAAGGCTGCCGCCCCAGCGGGGCAGCAGGTACACAGCAAGGGCCTGGATCGCTTCGAAGC‑AGCCGGGGCGGGCGATCAGCGCATGGCGCAGCCAGTAATAGCGAGGGTCCTCGTTTTCATGGTCGACCCGCTTGCCCAGATAGCGCGGCAGCGACTGTGGC  >  NC_002947/5970385‑5970670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑