Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,980,182 G→A 5.6% R276Q (CGG→CAG)  PP_5242 → GAF domain‑containing sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,980,1820GA 5.6% 216.6 / 2.0 71R276Q (CGG→CAG) PP_5242GAF domain‑containing sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (32/35);  new base A (2/2);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

TGGTGTGCAGCCGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAAC  >  NC_002947/5980036‑5980328
                                                                                                                                                  |                                                                                                                                                  
tGGTGTGCAGCCGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGCCTTGGCTCACTGCCAGCGGc                                                                                                                                                  >  4:6193/1‑149 (MQ=255)
  gtgtGCAGCCGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAg                                                                                                                                                >  2:8376/1‑149 (MQ=255)
     tgCAGCCGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTgg                                                                                                                                             <  6:73174/149‑1 (MQ=255)
     tgCAGCCGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTgg                                                                                                                                             <  4:150768/149‑1 (MQ=255)
       cAGCCGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTggg                                                                                                                                            <  1:8376/148‑1 (MQ=255)
          ccGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGgccgc                                                                                                                                        >  4:120491/1‑149 (MQ=255)
              gggcggCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCcac                                                                                                                                    <  6:137446/149‑1 (MQ=255)
                    ccAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGgccgcc                                                                                                                                       <  8:184802/140‑1 (MQ=255)
                    ccAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCGTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGgccgcc                                                                                                                                       >  7:184802/1‑140 (MQ=255)
                      aGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCAt                                                                                                                            >  6:290191/1‑149 (MQ=255)
                       gCGAAGCGGACAGCAAGGTTCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCTCAAATCCATg                                                                                                                           <  6:97028/149‑1 (MQ=255)
                       gCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATg                                                                                                                           <  2:215902/149‑1 (MQ=255)
                            gCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCAcc                                                                                                                      <  8:50194/149‑1 (MQ=255)
                            gCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGACCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCAcc                                                                                                                      >  7:221840/1‑149 (MQ=255)
                             cGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCAccc                                                                                                                     <  3:120491/149‑1 (MQ=255)
                               gACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCtt                                                                                                                   >  8:72125/1‑149 (MQ=255)
                                    cAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCAccc                                                                                                              >  1:113634/1‑149 (MQ=255)
                                     aaGGTGCCGCACTCCCTCAGCCGGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGAGTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCAcccc                                                                                                             <  6:323956/149‑1 (MQ=255)
                                           ccGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTg                                                                                                       >  5:95752/1‑149 (MQ=255)
                                            cGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTgc                                                                                                      <  4:275017/149‑1 (MQ=255)
                                              cACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTgcgc                                                                                                    <  5:286305/149‑1 (MQ=255)
                                               aCTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGTGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGcc                                                                                                   >  4:74382/1‑149 (MQ=255)
                                                   ccTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCGCTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCAc                                                                                                                       >  2:147539/1‑125 (MQ=255)
                                                   ccTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCGCTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCAc                                                                                                                       <  1:147539/125‑1 (MQ=255)
                                                   ccTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGAcc                                                                                               <  2:86124/149‑1 (MQ=255)
                                                         ccTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAg                                                                                         >  6:159043/1‑149 (MQ=255)
                                                             gTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAgcg                                                                                       <  6:95752/147‑1 (MQ=255)
                                                              tCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGccc                                                                                    >  4:68943/1‑149 (MQ=255)
                                                              accTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGccc                                                                                    <  8:221840/148‑1 (MQ=255)
                                                                   gCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCAGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCa                                                                               >  8:20370/1‑149 (MQ=255)
                                                                   gCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCAGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCa                                                                               >  8:25392/1‑149 (MQ=255)
                                                                        ggCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCAcgcgc                                                                          >  2:283583/1‑149 (MQ=255)
                                                                            ttGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACaa                                                                                                                                  >  6:76795/1‑89 (MQ=255)
                                                                            ttGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACaa                                                                                                                                  <  5:76795/89‑1 (MQ=255)
                                                                               gCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCAccc                                                                                                                     <  1:201906/99‑1 (MQ=255)
                                                                               gCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCAccc                                                                                                                     >  2:201906/1‑99 (MQ=255)
                                                                               gCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGtt                                                                   <  3:68943/149‑1 (MQ=255)
                                                                                ccAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGttt                                                                  <  2:119225/149‑1 (MQ=255)
                                                                                 cAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTg                                                                 <  2:113634/149‑1 (MQ=255)
                                                                                 cAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTg                                                                 >  5:207698/1‑149 (MQ=255)
                                                                                 cAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTg                                                                 >  7:44886/1‑149 (MQ=255)
                                                                                     gagCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCcg                                                             <  5:159043/149‑1 (MQ=255)
                                                                                         cGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCgcggc                                                         <  3:212278/149‑1 (MQ=255)
                                                                                                      ccAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGcc                                                                                     <  1:163071/108‑1 (MQ=255)
                                                                                                      ccAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGcc                                                                                     >  2:163071/1‑108 (MQ=255)
                                                                                                       cAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGc                                           >  1:340620/1‑149 (MQ=255)
                                                                                                       cAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGACGCGGCGCCGAGCAAAAGGc                                           >  8:222815/1‑149 (MQ=255)
                                                                                                         gagCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTa                                         <  7:72125/149‑1 (MQ=255)
                                                                                                         gagCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTa                                         <  8:44886/149‑1 (MQ=255)
                                                                                                          agCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGCCCCGGTAGCGCCCGGCCACGCGCGCCTGGTTGGCCGCGGCGCCGCGCCAAAGGCTag                                        >  3:27876/1‑148 (MQ=255)
                                                                                                           gcgcgcgcgcCTTTGCAGTGGACTTGGCTCACTGCCAGCAGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATc                                       <  7:25392/149‑1 (MQ=255)
                                                                                                           gcgcgcgcgcCTTTGCAGTGGACTTGGCTCACTGCCAGCAGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATc                                       <  7:20370/149‑1 (MQ=255)
                                                                                                                cgcgcCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCct                                  <  3:6193/149‑1 (MQ=255)
                                                                                                                 gcgcCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCctg                                 >  8:129482/1‑149 (MQ=255)
                                                                                                                 gcgcCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCctg                                 <  3:147045/149‑1 (MQ=255)
                                                                                                                         gCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGa                                                    >  2:113837/1‑122 (MQ=255)
                                                                                                                         gCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGa                                                    <  1:113837/122‑1 (MQ=255)
                                                                                                                          cAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAg                        <  5:290191/149‑1 (MQ=255)
                                                                                                                           aGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAgg                       <  7:129482/149‑1 (MQ=255)
                                                                                                                                aCTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAACCCATGGCACCCTTCACCCCAGGCTGCGCCGCCCCGGTAGCGCCCGGCCCCGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCtt                   >  5:113943/1‑148 (MQ=255)
                                                                                                                                 cTTGGCTCACTGCCAGTGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGa                 <  3:74382/149‑1 (MQ=255)
                                                                                                                                      cTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCCGGCTGCGCCGACCCGGTAGCGCCCGGCCCCGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATcgc            >  8:90663/1‑149 (MQ=255)
                                                                                                                                      cTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACGCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATcgc            <  7:222815/149‑1 (MQ=255)
                                                                                                                                       tCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATcgc            >  5:109139/1‑148 (MQ=255)
                                                                                                                                       tCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATcgc            >  3:36426/1‑148 (MQ=255)
                                                                                                                                        cACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGa          >  8:67509/1‑149 (MQ=255)
                                                                                                                                         aCTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGa          <  6:109139/148‑1 (MQ=255)
                                                                                                                                          cTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGa          >  4:251248/1‑147 (MQ=255)
                                                                                                                                            gCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAg       >  3:287166/1‑148 (MQ=255)
                                                                                                                                              cAGCGGCAGTGGGCCGCCCACAAAACCATGGCCCCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTGGAATCGCGATAGCaa    >  8:313660/1‑149 (MQ=255)
                                                                                                                                                gCGGCAGTGGGCCGCGCACAAAACCATGGCAACCTTCACCCCTGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAATGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGGAAAc  <  4:27876/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGGTGTGCAGCCGCGGGCGGCCAGCGAAGCGGACAGCAAGGTGCCGCACTCCCTCAGCCTGGTCCTGGCAAAGGCCTTGGCCAAAGAGCCGGACGCCCGCTACCAGAGCGCGCGCGCCTTTGCAGTGGACTTGGCTCACTGCCAGCGGCAGTGGGCCGCCCACAAAACCATGGCACCCTTCACCCCAGGCTGCGCCGACCCGGTAGCGCCCGGCCACGCGCGCCTGTTTGGCCGCGGCGCCGAGCAAAAGGCTATCACCCTGCTGCTCAAGGCCTTGAATCGCGATAGCAAAC  >  NC_002947/5980036‑5980328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑