Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 503,813 A→G 9.4% intergenic (+16/‑135) PP_0414 → / → rpe polyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947503,8130AG 9.4% 263.6 / 14.4 96intergenic (+16/‑135)PP_0414/rpepolyamine ABC transporter permease/ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (43/44);  new base G (3/6);  total (46/50)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TATTCGCGAGAACCTGAGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACG  >  NC_002947/503665‑503960
                                                                                                                                                     |                                                                                                                                                     
tATTCGCGAGAACCTGAGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTa                                                                                                                                                       <  6:328388/149‑1 (MQ=255)
     gcgAGAACCTGAGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGc                                                                                                                                                  >  8:287646/1‑149 (MQ=255)
       gagaACCTGAGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTGCCGGcc                                                                                                                                                 >  8:54827/1‑148 (MQ=255)
          aaCCTGAGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCtt                                                                                                                                             <  1:257852/149‑1 (MQ=255)
            ccTGAGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTCGGGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTc                                                                                                                                            >  1:85916/1‑149 (MQ=255)
               gAGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑gg                                                                                                                                       >  8:199750/1‑149 (MQ=255)
                aGCCCGACGTTTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGGGTTGTGGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGc                                                                                                                                      <  5:76988/149‑1 (MQ=255)
                aGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGc                                                                                                                                      >  4:98683/1‑149 (MQ=255)
                   ccGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTa                                                                                                                                    >  7:8383/1‑148 (MQ=255)
                     gACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAg                                                                                                                                 >  7:238087/1‑149 (MQ=255)
                         aTTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCg                                                                                                                              >  1:189276/1‑148 (MQ=255)
                            gCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑Gaa                                                                                                                          <  1:12455/148‑1 (MQ=255)
                            gCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑Gaa                                                                                                                          <  1:335861/148‑1 (MQ=255)
                             ccGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTGCCGGCCTCTTC‑C‑GGCTTTAGCCGCGAaga                                                                                                                        <  7:285886/149‑1 (MQ=39)
                              cgcgGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCGGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTGCCGGCCTCTTC‑C‑GGCTTTAGCCGCGAagag                                                                                                                       <  5:242482/149‑1 (MQ=38)
                                cggcggcgACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTTGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTGCCGGCCTCTTC‑C‑GGCTTTAGCCGCGAagag                                                                                                                       <  5:139090/147‑1 (MQ=38)
                                cggcggcgACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTGCCGGCCTCTTC‑C‑GGCTTTAGCCGCGAagag                                                                                                                       >  6:139090/1‑147 (MQ=39)
                                cggcggcgACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTGCCGGCCTCTTC‑C‑GGCTTTAGCCGCGAAGAGGc                                                                                                                     <  7:54827/149‑1 (MQ=38)
                                cggcggcgACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGc                                                                                                                     <  8:238087/149‑1 (MQ=255)
                                 ggcggcgACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTGCCGGCCTCTTC‑C‑GGCTTTAGCCGCGAAGAGGcc                                                                                                                    <  4:223794/149‑1 (MQ=38)
                                  gcggcgACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCg                                                                                                                   <  7:287646/149‑1 (MQ=255)
                                       gACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGcaca                                                                                                              <  7:199750/149‑1 (MQ=255)
                                          cTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGt                                                                                                           >  5:129630/1‑149 (MQ=255)
                                           ttGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGtt                                                                                                          >  3:247203/1‑149 (MQ=255)
                                               tGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGt                                                                                                      >  1:242508/1‑149 (MQ=255)
                                                gATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGtt                                                                                                     >  6:264100/1‑149 (MQ=255)
                                                gATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGT‑GGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTg                                                                                                    <  5:64487/149‑1 (MQ=255)
                                                  ttGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTgg                                                                                                   >  7:185280/1‑149 (MQ=255)
                                                     cGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc                                                                                                 <  2:189276/148‑1 (MQ=255)
                                                     cGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc                                                                                                 <  8:8383/148‑1 (MQ=255)
                                                     cGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTc                                                                                                 <  2:74971/148‑1 (MQ=255)
                                                      gTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGc                                                                                                                                                  <  7:159576/100‑1 (MQ=255)
                                                      gTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGc                                                                                                                                                  >  8:159576/1‑100 (MQ=255)
                                                      gTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGt                                                                                                      <  3:85307/142‑1 (MQ=255)
                                                      gTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGt                                                                                                      >  4:85307/1‑142 (MQ=255)
                                                        tctc‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACCGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGaca                                                                                             >  6:81452/1‑149 (MQ=255)
                                                         ctc‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGaca                                                                                             <  3:131605/148‑1 (MQ=255)
                                                          tc‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGACAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGacac                                                                                            >  6:1196/1‑148 (MQ=255)
                                                              gtgtTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑Gaa                                                                                                                          >  3:264178/1‑115 (MQ=255)
                                                              gtgtTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑Gaa                                                                                                                          <  4:264178/115‑1 (MQ=255)
                                                              gtgtTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAAt                                                                                        >  6:10961/1‑149 (MQ=255)
                                                              gtgtTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAAt                                                                                        >  3:83541/1‑149 (MQ=255)
                                                              gtgtTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAAt                                                                                        >  5:47993/1‑149 (MQ=255)
                                                                gttgttGCTTCTGACCGTGGAGTGGTTGGGTGGGCGTAGCGATAAGCTGAGGACTCAACAGCCGGCTTGATGTTGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑TAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAAtt                                                                                       <  2:242508/148‑1 (MQ=255)
                                                                 ttgttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGa                                                                                     <  4:223088/149‑1 (MQ=255)
                                                                   gttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGAt                                                                                    <  4:83541/148‑1 (MQ=255)
                                                                    ttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATa                                                                                   <  7:48544/148‑1 (MQ=255)
                                                                    ttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAc                                                                                  <  3:25648/149‑1 (MQ=255)
                                                                    ttgCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAc                                                                                  <  5:10961/149‑1 (MQ=255)
                                                                     tgctgcTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAc                                                                                  <  2:85916/148‑1 (MQ=255)
                                                                      gctgctGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATAcc                                                                                 >  5:163396/1‑148 (MQ=255)
                                                                         gctgACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGa                                                                                     <  5:90665/141‑1 (MQ=255)
                                                                         gctgACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGa                                                                                     >  6:90665/1‑141 (MQ=255)
                                                                                  ggAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGAt                                                                                    >  2:229977/1‑133 (MQ=255)
                                                                                  ggAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGAt                                                                                    <  1:229977/133‑1 (MQ=255)
                                                                                   gAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATc                                                                   <  6:47993/149‑1 (MQ=255)
                                                                                        gTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCtt                                                               <  1:304560/148‑1 (MQ=255)
                                                                                            cGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTccc                                                           <  6:163396/148‑1 (MQ=255)
                                                                                             gTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGAt                                                                                    >  5:188887/1‑122 (MQ=255)
                                                                                             gTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGAt                                                                                    <  6:188887/122‑1 (MQ=255)
                                                                                             gTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCt                                                          >  6:87032/1‑148 (MQ=255)
                                                                                              tGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGa                                                        <  4:247203/149‑1 (MQ=255)
                                                                                                    tAGCGAGAAGCTGAGGACTCAACAGCCTGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACa                                                  <  8:272257/149‑1 (MQ=255)
                                                                                                      gCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAAt                                                <  6:129630/149‑1 (MQ=255)
                                                                                                               tGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCAc                                       >  1:112188/1‑149 (MQ=255)
                                                                                                                gAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCAcc                                      >  3:223863/1‑149 (MQ=255)
                                                                                                                 aGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCAc                                       <  5:264100/147‑1 (MQ=255)
                                                                                                                      tCAACAGCCGGTTTGATGATGGCTTGCCTGTACCGGCCTCTTCGCGGGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCCACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGaa                                 >  1:250052/1‑149 (MQ=255)
                                                                                                                      tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCg                                                                                                       <  3:115628/78‑1 (MQ=255)
                                                                                                                      tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCg                                                                                                       >  4:115628/1‑78 (MQ=255)
                                                                                                                      tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGGGTTACAATgc                                              >  4:228099/1‑135 (MQ=255)
                                                                                                                      tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGGGTTACAATgc                                              <  3:228099/135‑1 (MQ=255)
                                                                                                                      tCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAt                                >  3:255874/1‑149 (MQ=255)
                                                                                                                        aaCAGCGGGCTTGATGATGGATTGCCGGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGATGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt                              <  4:255874/149‑1 (MQ=255)
                                                                                                                        aaCAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAttt                              <  5:1196/149‑1 (MQ=255)
                                                                                                                        aaCAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAAt                                <  3:98683/147‑1 (MQ=255)
                                                                                                                        aaCAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCAGAGTTACAATtcgc                                            >  4:338670/1‑135 (MQ=255)
                                                                                                                          cAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGc                            <  2:112188/149‑1 (MQ=255)
                                                                                                                             ccGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCaa                         >  7:182620/1‑149 (MQ=255)
                                                                                                                               ggCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTcc                                                            >  4:168215/1‑112 (MQ=255)
                                                                                                                               ggCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTcc                                                            <  3:168215/112‑1 (MQ=255)
                                                                                                                                gtttGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAg                      <  2:250052/147‑1 (MQ=255)
                                                                                                                                      tgatgGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTgcgc                >  7:239131/1‑149 (MQ=255)
                                                                                                                                      tgatgGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGCGTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTgcgc                >  4:100143/1‑149 (MQ=255)
                                                                                                                                      tgatgGCTTGCCTGTACCGGCCCCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTgcgc                >  8:113550/1‑149 (MQ=255)
                                                                                                                                        atgGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCa              >  7:234384/1‑149 (MQ=255)
                                                                                                                                           gCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGc           <  8:182620/149‑1 (MQ=255)
                                                                                                                                            cTTGCCTGTACCGGCCTCTTCGC‑GCCTTAAGCCG‑GAAGAGGCCGGCCCAGGTTTGGTTGGTGGACACCAATTGATACGAATCAGCGATCATCGCATTCCCTGAGTTAAAAAGGCTGGCACCGTGAATTTGCAAACAGGTGCGCCAtt            >  8:209596/1‑146 (MQ=255)
                                                                                                                                             ttGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTgcg                 >  4:302843/1‑141 (MQ=255)
                                                                                                                                             ttGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTgcg                 <  3:302843/141‑1 (MQ=255)
                                                                                                                                             ttGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAg         >  8:8348/1‑149 (MQ=255)
                                                                                                                                                ccTGTACCGGCCTCTTCGC‑GGCTAAAGCAG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGccc      >  1:19871/1‑149 (MQ=255)
                                                                                                                                                 cTGTGCCGGCCTCTTC‑C‑GGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAg                      <  3:132768/132‑1 (MQ=37)
                                                                                                                                                 cTGTGCCGGCCTCTTC‑C‑GGCTTTAGCCGCGAAGAGGCCGGTACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAg                      >  4:132768/1‑132 (MQ=37)
                                                                                                                                                 cTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCAcc                                      >  3:121356/1‑116 (MQ=255)
                                                                                                                                                 cTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCAcc                                      <  4:121356/116‑1 (MQ=255)
                                                                                                                                                    tACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACg  <  8:239131/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TATTCGCGAGAACCTGAGCCCGACCATTGCCGCGGCGGCGACCTTGCTGATTGCGTTCTC‑GGTGTTGTTGCTGCTGACCCTGGAGTGGTTGCGTGGGCGTAGCGAGAAGCTGAGGACTCAACAGCCGGCTTGATGATGGCTTGCCTGTACCGGCCTCTTCGC‑GGCTAAAGCCG‑GAAGAGGCCGGCACAGGTTGCGTTGGTCGACACCAATTGATACCAATCAGCCTTCATCGCTTTCCCTGAGTTACAATGCGCGCCACCGTGAATTTGCCAACAGGTGCGCCATGCAGCCCTACG  >  NC_002947/503665‑503960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑