Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,015,362 C→T 8.0% D135N (GAT→AAT)  PP_5266 ← acetyl‑CoA hydrolase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,015,3620CT 8.0% 141.0 / 2.5 50D135N (GAT→AAT) PP_5266acetyl‑CoA hydrolase family protein
Reads supporting (aligned to +/- strand):  ref base C (24/22);  new base T (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-01

CTGCAGCGCGGCGCTTGGCGATCATCGGCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGTGGCAACGTGTCGTTGCGCAGGTCTGCGAGGTAACTGAGC  >  NC_002947/6015224‑6015507
                                                                                                                                           |                                                                                                                                                 
cTGCAGCGCGGCGCTTGGCGATCATCGGCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCa                                                                                                                                                  <  1:345881/140‑1 (MQ=255)
cTGCAGCGCGGCGCTTGGCGATCATCGGCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCa                                                                                                                                                  >  2:345881/1‑140 (MQ=255)
 tGCAGCGCGGCGCTTGGCGATCATCGGCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGt                                                                                                                                        >  5:110909/1‑149 (MQ=255)
   cAGCGCGGCGCTTGGCGATCATCGGCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGt                                                                                                                                        <  6:16316/147‑1 (MQ=255)
   cAGCGCGGCGCTTGGCGATCATCGGCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTgg                                                                                                                                      <  3:28399/149‑1 (MQ=255)
   cAGCGCGGCGCTTGGCGATCATCGGCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTgg                                                                                                                                      <  6:110909/149‑1 (MQ=255)
            gcTTGGCGATCATCGGCAGCAGGTCGAGGGTGATGTGTGGGTTGCATGCCAGGCTCATGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTtg                                                                                                                             <  5:157017/149‑1 (MQ=255)
                  cGATCATCGGCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGctgctg                                                                                                                       <  8:246699/149‑1 (MQ=255)
                     tcatcGGCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTg                                                                                                                                   >  2:245997/1‑134 (MQ=255)
                     tcatcGGCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCAATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTg                                                                                                                                   <  1:245997/134‑1 (MQ=255)
                        tcGGCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAg                                                                                                                 >  7:197221/1‑149 (MQ=255)
                          ggCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGt                                                                                                                <  2:150199/148‑1 (MQ=255)
                          ggCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTc                                                                                                               >  8:202720/1‑149 (MQ=255)
                          ggCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTc                                                                                                               <  3:256714/149‑1 (MQ=255)
                           gcagcaGGTCGAGGGTTATGTCTGGTTTGCAGGCCAGGCTCAGGTTCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTtg                                                                                                                             <  3:208608/134‑1 (MQ=255)
                           gcagcaGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGGGTGGCTGTAGTTGCTGCTGACGTAGTcc                                                                                                              >  3:160038/1‑149 (MQ=255)
                                  gTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTggg                                                                                                       >  6:134802/1‑149 (MQ=255)
                                    cGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGTGGCCA‑CCAACTGGGCTATCAGGTTCAGGCGCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGc                                                                                                      <  3:269083/148‑1 (MQ=255)
                                    cGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGTGGCCA‑CCAACTGGGCTATCAGGTTCAGGCGCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGc                                                                                                      >  4:269083/1‑148 (MQ=255)
                                               tCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCtgtg                                                                                          >  8:276540/1‑149 (MQ=255)
                                                   ggTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGGGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTgcagc                                                                                      <  4:160038/149‑1 (MQ=255)
                                                     ttGCAGGCCAGGCTCAGGTGAACGGGCTTTACGGGGGTGGCGGCCA‑CCAACTGCGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGCCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGAATCGCTGTGcagcag                                                                                    >  3:296924/1‑149 (MQ=255)
                                                        caggccaggcTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCt                                                                                 <  8:197221/149‑1 (MQ=255)
                                                         aggccaggcTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAg                                                                                                                                >  7:67252/1‑101 (MQ=255)
                                                         aggccaggcTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAg                                                                                                                                <  8:67252/101‑1 (MQ=255)
                                                         aggccaggcTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTg                                                                                >  1:342619/1‑149 (MQ=255)
                                                                   cAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCa                                                                      >  3:161564/1‑149 (MQ=255)
                                                                        gCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATg                                                                    >  7:258981/1‑146 (MQ=255)
                                                                         cACGGGCTTTTCGGGGGTGGCGGCCAGCCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATTACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATg                                                                    >  1:3880/1‑146 (MQ=255)
                                                                         cACGGGCTTTTCGGGGGTGGCGGCCAGCCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATTACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATg                                                                    >  1:9422/1‑146 (MQ=255)
                                                                         cACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATgaag                                                                 >  5:313124/1‑148 (MQ=255)
                                                                               cTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGtt                                                          <  4:161564/149‑1 (MQ=255)
                                                                                     ggggtTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGGTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGAccc                                                    <  8:282480/149‑1 (MQ=255)
                                                                                            gcggcCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATAtt                                             >  2:10294/1‑149 (MQ=255)
                                                                                             cggcCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTg                                            <  1:10294/149‑1 (MQ=255)
                                                                                              ggcCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATTACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTgg                                           <  2:9422/149‑1 (MQ=255)
                                                                                              ggcCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATTACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTgg                                           <  2:3880/149‑1 (MQ=255)
                                                                                                cca‑ccaACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGt                                         >  3:113249/1‑149 (MQ=255)
                                                                                                 ca‑ccaACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGTg                                        >  4:40038/1‑149 (MQ=255)
                                                                                                  a‑ccaACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGTgg                                       <  6:313124/149‑1 (MQ=255)
                                                                                                     caACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGTGGCa                                     <  2:342619/149‑1 (MQ=255)
                                                                                                                tCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGTGGCAACGTGTCGTTg                          >  7:126637/1‑149 (MQ=255)
                                                                                                                    gTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGTGGCAACGACACGTTgccac                      <  7:276540/149‑4 (MQ=255)
                                                                                                                       cAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGTGGCAACGTGTCGTTGCGCAGGt                   >  4:240915/1‑149 (MQ=255)
                                                                                                                                      gATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGTGGCAACGTGTCGTTGCGCAGGTCt                 <  3:23047/136‑1 (MQ=255)
                                                                                                                                      gATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGTGGCAACGTGTCGTTGCGCAGGTCt                 >  4:23047/1‑136 (MQ=255)
                                                                                                                                       atatCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGTGGCAACGTGTCGTTGCGCAGGTCTGCGAGGTAACTGAg   >  7:94506/1‑149 (MQ=255)
                                                                                                                                        tatCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGTGGCAACGTGTCGTTGCGCAGGTCTGCGAGGTAACTGAGc  >  5:334696/1‑149 (MQ=255)
                                                                                                                                         atCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGc                                                                       <  6:244609/79‑1 (MQ=255)
                                                                                                                                         atCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGc                                                                       >  5:244609/1‑79 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
CTGCAGCGCGGCGCTTGGCGATCATCGGCAGCAGGTCGAGGGTGATGTCTGGGTTGCAGGCCAGGCTCAGGTGCACGGGCTTTTCGGGGGTGGCGGCCA‑CCAACTGGGCTATCAGGTTCAGGCCCTTGGCGTTGATATCACGTGCGGCGTGGCTGTAGTTGCTGCTGACGTAGTCCTGCTGGGCCGATTCGCTGTGCAGCAGGCTGCCGGGCTGCATGAAGAACTGTTCGACCCGAATATTGGGTGGCAACGTGTCGTTGCGCAGGTCTGCGAGGTAACTGAGC  >  NC_002947/6015224‑6015507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑