Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,111,197	C→G	7.8%	E105Q (GAG→CAG)	PP_5361 ←	protein P47K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,111,197	0	C	G	7.8%	191.1 / 6.9	64	E105Q (GAG→CAG)	PP_5361	protein P47K
Reads supporting (aligned to +/- strand):  ref base C (29/29);  new base G (3/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

CTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCAGCGCGGTTGAGGCT  >  NC_002947/6111052‑6111344                                                                                                                                                    |                                                                                                                                                    cTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCaga                                                                                                                                                  <  7:106601/149‑1 (MQ=255)   ggCCAGGCTGGCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCTGAGCCACTGCCAGTGGCTgaga                                                                                                                                                  <  6:287374/149‑1 (MQ=38)      cAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATAc                                                                                                                                             <  4:153961/149‑1 (MQ=255)      cAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATAc                                                                                                                                             >  4:57431/1‑149 (MQ=255)        ggCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCg                                                                                                                                           <  1:154113/149‑1 (MQ=255)             tCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTg                                                                                                                                        <  2:295683/147‑1 (MQ=255)             tCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGa                                                                                                                                      >  3:179490/1‑149 (MQ=255)                    ccTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGCCCATGGTGTCCAGTCGCGCCATATCGGACAGCCTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑CGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGAt                                                                                                                                >  1:315788/1‑148 (MQ=255)                    ccTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGCGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGa                                                                                                                                 <  6:196401/147‑1 (MQ=255)                     cTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGAt                                                                                                                                >  1:273418/1‑147 (MQ=255)                       gATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCaga                                                                                                                                                  <  1:231464/127‑1 (MQ=255)                       gATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCaga                                                                                                                                                  >  2:231464/1‑127 (MQ=255)                                    gAAGTTCAAGCCATCGCCCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGCCAGCGCCCCTGCTCGTCGCGGACAGTGAAGGTCTC‑‑AGCCCCTGGCAGTGGCTCAGCGAGACCGGTGGCCGCGATCAGCAAAGAATCGaacc                                                                                                               >  2:169709/1‑147 (MQ=255)                                    gAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGCCAGTGGCTGag                                                                                                                                                   <  7:158247/113‑1 (MQ=255)                                    gAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGCCAGTGGCTGag                                                                                                                                                   >  8:158247/1‑113 (MQ=255)                                        ttCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGccc                                                                                                           <  3:57431/149‑1 (MQ=255)                                         tCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGccc                                                                                                           <  8:164240/148‑1 (MQ=255)                                           aaGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGAATGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCctc                                                                                                        >  6:92293/1‑149 (MQ=255)                                             gCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGc                                                                                                             <  4:301616/142‑1 (MQ=255)                                             gCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGc                                                                                                             >  3:301616/1‑142 (MQ=255)                                             gCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCtt                                                                                                      >  2:219113/1‑149 (MQ=255)                                               cATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCg                                                                                                    >  3:3510/1‑149 (MQ=255)                                                aTCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCgg                                                                                                   <  2:273418/149‑1 (MQ=255)                                                 tCGAACACGGTGACCATGGTGTCCAGTCGCGCAATATCGGACAGAATGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGATAAGAGATACCGGTGGACTCGATCAGCAAATAATCg                                                                                                                   >  5:29091/1‑132 (MQ=255)                                                      cACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCTGTGGCTCAGAGATACCGGTTGACTCGATCAGCAAATAATCGAAACGc                                                                                                             <  3:16335/133‑1 (MQ=255)                                                      cACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATAAGCAAATAATCGAAACga                                                                                                             >  4:16335/1‑132 (MQ=255)                                                                   gtgtCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACctcctc                                                                                <  8:120559/149‑1 (MQ=255)                                                                        cAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCa                                                                                                >  1:168159/1‑128 (MQ=255)                                                                        cAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCa                                                                                                <  2:168159/128‑1 (MQ=255)                                                                        cAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAg                                                                             >  4:307983/1‑147 (MQ=255)                                                                        cAGGCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGG‑CAGTCGCGCAACCTCCTCGAGCAg                                                                          <  8:42692/149‑1 (MQ=255)                                                                         aGTCGCGCCATATCGGCCAGACGGCGACCCTGCGCGGCGCGGAAAGTGAAGGGCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAATCGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAg                                                                          <  1:169709/149‑1 (MQ=255)                                                                                ccATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGc                                                                                                                            >  1:7184/1‑92 (MQ=255)                                                                                ccATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGc                                                                                                                            <  2:7184/92‑1 (MQ=255)                                                                                       ggACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAAc                                                             <  8:197079/148‑1 (MQ=255)                                                                                        gACAGACTGCGACCCTGATCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATAAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGAGCAACCTCCACGAGCAGGTCTTCGCGCAAcct                                                           >  2:58376/1‑147 (MQ=255)                                                                                          cAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTgc                                                         <  3:307983/149‑1 (MQ=255)                                                                                          cAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTgc                                                         <  5:92293/149‑1 (MQ=255)                                                                                          cAGAATGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑ATCCACTGGCAGTGGCTCAGAGATACCGTTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTgc                                                         <  1:58376/149‑1 (MQ=255)                                                                                            gACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTgcag                                                       <  4:3510/149‑1 (MQ=255)                                                                                            gACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTgc                                                         <  5:201983/147‑1 (MQ=255)                                                                                            gACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTgc                                                         >  6:201983/1‑147 (MQ=255)                                                                                                 cGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCACCCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAAt                                                  >  6:103310/1‑149 (MQ=255)                                                                                                  gACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATg                                                 <  2:9221/149‑1 (MQ=255)                                                                                                    cccTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATg                                                 >  1:83457/1‑147 (MQ=255)                                                                                                            gtcgCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGc                                       >  8:260419/1‑149 (MQ=255)                                                                                                                       ggtatctctg‑‑AGCCACTGCCAGTGGCTGAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGAc                             >  3:161875/7‑148 (MQ=255)                                                                                                                             tctg‑‑AGCCACTGCCAGTGGCTGGGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTtc                      >  5:115087/1‑149 (MQ=255)                                                                                                                               tc‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGCCCAGTTtctc                    >  6:201298/1‑149 (MQ=255)                                                                                                                               tc‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTtctc                    >  6:79620/1‑149 (MQ=255)                                                                                                                                   aGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTtctc                    <  7:260419/147‑1 (MQ=255)                                                                                                                                     ccACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCa                <  1:219113/149‑1 (MQ=255)                                                                                                                                     ccACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGGCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCa                <  2:315788/149‑1 (MQ=255)                                                                                                                                     ccACTGCCAGTGGCTGGGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCa                <  6:115087/149‑1 (MQ=255)                                                                                                                                       aCTGGCAGTGGCTCAGAGATACCGGTGGACTCGCTCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCAgc              >  1:32166/1‑149 (MQ=255)                                                                                                                                         tGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCAgcgc            >  2:329503/1‑149 (MQ=255)                                                                                                                                           gCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCAGCGCgg          >  2:164956/1‑149 (MQ=255)                                                                                                                                             aGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTgca                                                        >  6:18061/1‑101 (MQ=255)                                                                                                                                             aGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCAGCGCGGtt        >  4:196338/1‑149 (MQ=255)                                                                                                                                                 gCTCAGCGATCCCGTGGGCCTCGATCAGCCAATACACGAAACGCCCCGCTTCGTCCCGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGGGCAGCAAATGCAGCCTTTGCTCATCTCGACCATTTTCTCTTCAGCGCGGTTGAg     <  6:349816/148‑1 (MQ=255)                                                                                                                                                 gCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGttt                       >  7:291014/1‑130 (MQ=255)                                                                                                                                                 gCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGttt                       <  8:291014/130‑1 (MQ=255)                                                                                                                                                   tCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCACCCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCAGCGCGGTTGAGGCt  >  8:92538/1‑149 (MQ=255)                                                                                                                                                   tCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCACCCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCAGCGCGGTTGAGGCt  >  6:329584/1‑149 (MQ=255)                                                                                                                                                   tCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTg                                        <  2:173582/111‑1 (MQ=255)                                                                                                                                                    cAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCAGCGCGGTTGAGGCt  >  1:183019/1‑148 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTC‑‑AGCCACTGGCAGTGGCTCAGAGATACCGGTGGACTCGATCAGCAAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCAGCGCGGTTGAGGCT  >  NC_002947/6111052‑6111344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑