Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,127,163 A→T 8.7% V241E (GTG→GAG) ‡ PP_5375 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,127,1630AT 8.7% 200.0 / 8.2 77V241E (GTG→GAG) ‡PP_5375LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (37/33);  new base T (2/5);  total (39/38)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01

AACAGCCGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGC  >  NC_002947/6127016‑6127309
                                                                                                                                                   |                                                                                                                                                  
aaCAGCCGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCAc                                                                                                                                                   >  7:259326/1‑149 (MQ=255)
     ccGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGGCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCt                                                                                                                                              <  7:258837/149‑1 (MQ=255)
       gCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTgg                                                                                                                                            >  2:185550/1‑149 (MQ=255)
           gatttCACGATGACCAAGCTCTGTGGAAGACTCCAGCAGGCAGAACTTGTCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGcctg                                                                                                                                        <  6:239133/147‑3 (MQ=255)
           gCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGcctg                                                                                                                                        <  5:108256/149‑3 (MQ=255)
            cTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGcctgg                                                                                                                                       <  3:285616/149‑4 (MQ=255)
             tttCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACt                                                                                                                                       >  4:149319/1‑148 (MQ=255)
               tCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTg                                                                                                                                      <  6:162038/147‑1 (MQ=255)
               tCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGa                                                                                                                                     <  7:73097/148‑1 (MQ=255)
                         cACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCTCCGACTg                                                                                                                           >  6:300844/1‑148 (MQ=255)
                         cACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTg                                                                                                                           >  1:150151/1‑148 (MQ=255)
                                      cgacTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCGACCAGGTTGCCCCAGGCCAGGGCCACGCCATGGCCGCTGATCGCCGACTGGATCAGCCTACCg                                                                                                              >  8:154540/1‑148 (MQ=255)
                                      cgacTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGAt                                                                                                                                    <  1:141379/126‑1 (MQ=255)
                                      cgacTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGAt                                                                                                                                    >  2:141379/1‑126 (MQ=255)
                                           ccAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTg                                                                                                        >  3:17118/1‑149 (MQ=255)
                                            cagcagGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGt                                                                                                       <  8:259326/149‑1 (MQ=255)
                                             agcagGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGtt                                                                                                      <  7:91476/149‑1 (MQ=255)
                                             agcagGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATAACGTAGCTGtt                                                                                                      >  3:46927/1‑149 (MQ=255)
                                                  gcagAACTTGGCGTCGGTCTGGAGTACAACATCGTTGGGCTTGACCACCGCACCGGTGATCAACGCATCGTCCACCAGGTAGCCCCAGGCCAGGGCCACGCCCAGGCCACTCATCGCCGACTGGATCAGACTACAGTAGCTGTTGatat                                                                                                 >  8:181304/1‑149 (MQ=255)
                                                    agAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATAtt                                                                                                >  1:2848/1‑148 (MQ=255)
                                                    agAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATAACGAAGATGTTGATAtt                                                                                                >  5:70629/1‑148 (MQ=255)
                                                      aaCTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGAc                                                                                             <  2:20833/149‑1 (MQ=255)
                                                       aCTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGAcg                                                                                            >  2:336790/1‑149 (MQ=255)
                                                           ggCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGt                                                                                                             >  5:299222/1‑128 (MQ=255)
                                                           ggCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGt                                                                                                             <  6:299222/128‑1 (MQ=255)
                                                              gTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCt                                                                                     >  1:182769/1‑149 (MQ=255)
                                                                  gTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGGTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTggg                                                                                 <  5:328802/149‑1 (MQ=255)
                                                                   tCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgggg                                                                                >  4:201146/1‑149 (MQ=255)
                                                                   tCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgggg                                                                                <  2:150151/149‑1 (MQ=255)
                                                                   tCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgggg                                                                                <  3:149319/149‑1 (MQ=255)
                                                                       gAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACCCATCGTCCACCAGGTTGCCCCAGGCCAGGCCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCCAAGCAGTTGATATTGACGCGAAGCTTGGGGGTCa                                                                            >  5:29317/1‑149 (MQ=255)
                                                                         gTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCTATGCTTGGGGGTCATg                                                                          <  1:185550/149‑1 (MQ=255)
                                                                         gTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATg                                                                          <  6:278755/149‑1 (MQ=255)
                                                                               caTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATc                                                                                                                                   >  3:322062/1‑86 (MQ=255)
                                                                               caTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATc                                                                                                                                   <  4:322062/86‑1 (MQ=255)
                                                                               caTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCg                                                                    >  2:274164/1‑149 (MQ=255)
                                                                                 tCGTTGGGCTTGACCAGGGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGTTGGCCGACTGGATCAGGATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTa                                                                  <  7:181304/149‑1 (MQ=255)
                                                                                        gCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAgg                                                           <  5:300844/149‑1 (MQ=255)
                                                                                            gACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGgcgc                                                        <  1:336790/148‑1 (MQ=255)
                                                                                             aCCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTAt                                                                 >  6:16769/1‑138 (MQ=255)
                                                                                             aCCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTAt                                                                 <  5:16769/138‑1 (MQ=255)
                                                                                             aCCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCtt                                                      >  5:87371/1‑149 (MQ=255)
                                                                                               cAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgggg                                                                                <  6:288678/121‑1 (MQ=255)
                                                                                               cAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgggg                                                                                >  5:288678/1‑121 (MQ=255)
                                                                                                  cgcACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgg                                                                                  >  8:30464/1‑116 (MQ=255)
                                                                                                  cgcACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgg                                                                                  <  7:30464/116‑1 (MQ=255)
                                                                                                     aCCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCAtt                                              >  1:187238/1‑149 (MQ=255)
                                                                                                       cGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCg                                            >  4:25885/1‑149 (MQ=255)
                                                                                                        ggTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCgg                                           <  6:307885/149‑1 (MQ=255)
                                                                                                         gTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCgg                                           >  1:227824/1‑148 (MQ=255)
                                                                                                         gTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCgg                                           <  2:227824/148‑1 (MQ=255)
                                                                                                             tCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCaa                                      >  1:244435/1‑149 (MQ=255)
                                                                                                               aaCGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCaa                                      <  6:70629/147‑1 (MQ=255)
                                                                                                               aaCGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAAcc                                    <  2:2848/149‑1 (MQ=255)
                                                                                                               aaCGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAAc                                     <  4:17118/148‑1 (MQ=255)
                                                                                                               aaCGCATCCTCCACCAGGTCGCCCCAGGCAAGGGCCACGCCCTGGCCACTGCTCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGTGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGTCCAAc                                     <  6:23840/148‑1 (MQ=255)
                                                                                                                    aTCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACAGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTAcc                                >  5:82691/1‑148 (MQ=255)
                                                                                                                     tCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCa                              <  2:244435/149‑1 (MQ=255)
                                                                                                                           accaGGTTGACCCAGGCCAGGGCCACGCCCTGGCCAATGATCGCCGACTGGATAAGCATACAGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGAGCTTGAACCATTCTGGCCAAACTAAACAGtagag                        >  7:213769/1‑145 (MQ=255)
                                                                                                                              aGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGGTGATATTGACGCGATGCGTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCCCTg                     >  7:268333/1‑149 (MQ=255)
                                                                                                                                 ttGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGa                  <  2:239050/149‑1 (MQ=255)
                                                                                                                                 ttGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGa                  <  2:187238/149‑1 (MQ=255)
                                                                                                                                   gCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGt                >  1:177358/1‑149 (MQ=255)
                                                                                                                                    ccccAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACGGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTc               <  6:82691/149‑1 (MQ=255)
                                                                                                                                     cccAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCgg                                           >  8:290695/1‑120 (MQ=255)
                                                                                                                                     cccAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCgg                                           <  7:290695/120‑1 (MQ=255)
                                                                                                                                     cccAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCt              >  2:124865/1‑149 (MQ=255)
                                                                                                                                      ccaggccaggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCtt             >  3:244385/1‑149 (MQ=255)
                                                                                                                                        aggccaggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCa           <  1:124865/149‑1 (MQ=255)
                                                                                                                                         ggccaggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCaa          <  2:60709/149‑1 (MQ=255)
                                                                                                                                          gccaggGCGTGGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGa                  <  8:198966/140‑1 (MQ=255)
                                                                                                                                          gccaggGCGTGGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGa                  >  7:198966/1‑140 (MQ=255)
                                                                                                                                           ccaggGCGTGGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTg                                                     <  6:15479/104‑1 (MQ=255)
                                                                                                                                           ccaggGCGTGGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTg                                                     >  5:15479/1‑104 (MQ=255)
                                                                                                                                            caggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCACCCTACCCAGTCGCGCTGGGAGTCTTCAagc        >  7:95305/1‑148 (MQ=255)
                                                                                                                                                gCCACGCCCTGGCCACTGATCGCCGACTGCATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAagccagag   >  4:166897/1‑149 (MQ=255)
                                                                                                                                                 ccACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAagccagagc  >  7:175647/1‑149 (MQ=255)
                                                                                                                                                   aCGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAagccagagc  >  6:279054/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AACAGCCGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGC  >  NC_002947/6127016‑6127309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑