Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,169,790 T→A 5.7% S169C (AGC→TGC)  PP_5408 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,169,7900TA 5.7% 208.6 / 2.5 70S169C (AGC→TGC) PP_5408hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (37/28);  new base A (2/2);  total (39/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

TCTATGTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAATAAGA  >  NC_002947/6169645‑6169934
                                                                                                                                                 |                                                                                                                                                
tCTATGTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTgc                                                                                                                                                <  2:322637/148‑1 (MQ=255)
    tGTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGccc                                                                                                                                            <  2:283497/148‑1 (MQ=255)
     gTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCg                                                                                                                                           >  7:271657/1‑148 (MQ=255)
         tttAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTg                                                                                                                                                 >  2:54145/1‑138 (MQ=255)
         tttAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTg                                                                                                                                                 <  1:54145/138‑1 (MQ=255)
         tttAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCAGCCTAGGCAgcgcaagg                                                                                                                                          <  8:193491/145‑5 (MQ=255)
         tttAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCAGCCTAGGCAgcgcaagg                                                                                                                                          >  7:193491/1‑141 (MQ=255)
          ttAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCAGCCTAGGCAgcgcaa                                                                                                                                            >  6:138578/1‑140 (MQ=255)
          ttAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCAGCCTAGGCAgcgcaa                                                                                                                                            <  5:138578/142‑3 (MQ=255)
                tGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCt                                                                                                                                <  4:231257/148‑1 (MQ=255)
                 gCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTc                                                                                                                               <  2:236080/148‑1 (MQ=255)
                 gCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTc                                                                                                                               >  4:125708/1‑148 (MQ=255)
                  cTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCca                                                                                                                             <  4:283551/149‑1 (MQ=255)
                          cACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCt                                                                                                                     <  6:235784/149‑1 (MQ=255)
                               gTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGATCCACATTACCTGGa                                                                                                                  >  5:94320/1‑147 (MQ=255)
                                  gAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGccag                                                                                                                                           <  7:91398/119‑3 (MQ=255)
                                  gAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGccag                                                                                                                                           >  8:91398/1‑117 (MQ=255)
                                   aaCCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGt                                                                                                            >  3:19544/1‑149 (MQ=255)
                                       gTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGc                                                                                                         >  4:21701/1‑148 (MQ=255)
                                        tttCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCa                                                                                                       >  3:97116/1‑149 (MQ=255)
                                        tttCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATACTTCCGTATAATTGTGCCAGCCGCAATGCCAATGCGTCCCTGTTGACCTTATGCCTTCAGATGACTAGGCTTCGCCCGCAACATACGCTCCACATTACCTGTACAGAGTGGca                                                                                                        >  3:211949/1‑147 (MQ=255)
                                          tCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATc                                                                                                     >  8:46577/1‑149 (MQ=255)
                                              ttGCAAGGTGGTTCTAAAGGAGGCAGATCCTTCCTTAGAATTGTGCCAGCCGAAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCga                                                                                                 <  8:51067/148‑1 (MQ=255)
                                              gTGCATGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCga                                                                                                 <  8:271657/149‑1 (MQ=255)
                                                   aGGTGGTGATCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCAAGGCTGCGCCCGCAAAATCCGCTCCAAATTACCTTGACAGAGTGGCCATCGCGAGaaaa                                                                                            <  7:46577/149‑1 (MQ=255)
                                                        gtgCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGgct                                                                                       >  3:173346/1‑149 (MQ=255)
                                                          gCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGgctgc                                                                                     >  2:192492/1‑149 (MQ=255)
                                                           cTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCt                                                                                                                     <  1:6422/116‑1 (MQ=255)
                                                           cTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCt                                                                                                                     >  2:6422/1‑116 (MQ=255)
                                                              aaGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTca                                                                                  <  3:21701/148‑1 (MQ=255)
                                                              aaGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTca                                                                                  <  4:19544/148‑1 (MQ=255)
                                                                  aggCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGaa                                                                             <  1:192492/149‑1 (MQ=255)
                                                                      aGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTgg                                                                         <  4:97116/149‑1 (MQ=255)
                                                                      aGATCCTTCCGTAGAATTGTGCCAGCCGCAAGGACACTGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACTTCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTgg                                                                         <  7:299369/149‑1 (MQ=255)
                                                                      aGATCATTCCGTAGAATTGTGGCATCCGCAATGCCACTGGGTCGCTTGTTACCGTAGTCCTTGCGCTGCCTAGGCGGCGCCCGGAACATCCGCTCCTCATTACCTGTACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAgcaattg                                                                         <  4:211949/149‑7 (MQ=255)
                                                                           cTTCCGTAGAATTGTGCCAGCCGCAGTGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTa                                                                    >  1:218289/1‑149 (MQ=255)
                                                                            ttCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTAc                                                                   >  3:100417/1‑149 (MQ=255)
                                                                            ttCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTAc                                                                   >  3:76292/1‑149 (MQ=255)
                                                                               cGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTAc                                                                   >  7:54365/1‑146 (MQ=255)
                                                                                     aTTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCaa                                                           <  4:76292/148‑1 (MQ=255)
                                                                                            cAGCCGCAATGCCAATGGGTCCCTGTCGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGt                                                   >  2:1929/1‑149 (MQ=255)
                                                                                                  cAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGgctg                                                                                      >  8:102096/1‑108 (MQ=255)
                                                                                                  cAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGgctg                                                                                      <  7:102096/108‑1 (MQ=255)
                                                                                                    aTGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTc                                           <  8:54365/149‑1 (MQ=255)
                                                                                                         aaTGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACATGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTAAGAGAGCAAATCGACGTTGTGCTTCGCGca                                      >  3:194703/1‑149 (MQ=255)
                                                                                                              gTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAg                                 >  4:3371/1‑149 (MQ=255)
                                                                                                               tCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGt                                >  1:178690/1‑149 (MQ=255)
                                                                                                               tCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTCGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGt                                >  8:189800/1‑149 (MQ=255)
                                                                                                                 ccTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGcacaca                                  <  6:88920/145‑1 (MQ=255)
                                                                                                                 ccTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGcacaca                                  >  5:88920/1‑145 (MQ=255)
                                                                                                                  cTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTa                              >  2:104743/1‑148 (MQ=255)
                                                                                                                     ttGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACAtg                          >  5:220199/1‑149 (MQ=255)
                                                                                                                     ttGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACAt                           <  2:218289/148‑1 (MQ=255)
                                                                                                                        gCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTgg                       >  3:291034/1‑149 (MQ=255)
                                                                                                                        gCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTgg                       <  4:100417/149‑1 (MQ=255)
                                                                                                                             aGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTg                    >  5:26283/1‑147 (MQ=255)
                                                                                                                               gCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGa                >  1:187378/1‑149 (MQ=255)
                                                                                                                                 cTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACg              >  2:188104/1‑149 (MQ=255)
                                                                                                                                   tGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTc            >  2:244185/1‑149 (MQ=255)
                                                                                                                                      gcTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGcc         <  4:173346/149‑1 (MQ=255)
                                                                                                                                      gcTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGc          >  5:260583/1‑148 (MQ=255)
                                                                                                                                      gcTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGc          >  6:41511/1‑148 (MQ=255)
                                                                                                                                          ccTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATTGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAAt      >  8:306565/1‑148 (MQ=255)
                                                                                                                                          ccTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAAt      >  7:111675/1‑148 (MQ=255)
                                                                                                                                           cTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGt                                >  7:223066/1‑121 (MQ=255)
                                                                                                                                           cTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGt                                <  8:223066/121‑1 (MQ=255)
                                                                                                                                           cTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAAt      >  7:26014/1‑147 (MQ=255)
                                                                                                                                           cTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAATaa    <  2:187378/149‑1 (MQ=255)
                                                                                                                                           cTAGGCTGCGCCCGCAAAATCCTCTCCACATTACCTGGACCGAGTTGCCATCGCGAGAAAAGGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAATaa    <  4:194703/149‑1 (MQ=255)
                                                                                                                                              ggCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAATAaga  <  7:189800/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCTATGTTTTTTAGTTTGCTTTCGAGCACCCGTAGAACCGTTTCAAGTGCAAGGTGGTGCTCAAGGAGGCAGATCCTTCCGTAGAATTGTGCCAGCCGCAATGCCAATGGGTCCCTGTTGGCCGTAGGCCTTGCGCTGCCTAGGCTGCGCCCGCAACATCCGCTCCACATTACCTGGACAGAGTGGCCATCGCGAGAAAATGGCTGCTCACAGAACTGGCAGTACGAGAGCAAATCGACGTTGTGCTTCGCGCACACAGTTACATGTGGGTGAAGACGTCGCCAATAAGA  >  NC_002947/6169645‑6169934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑