Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,176,100 G→A 10.6% A166V (GCC→GTC)  atpD ← ATP synthase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,176,1000GA10.6% 178.7 / 9.3 66A166V (GCC→GTC) atpDATP synthase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (30/29);  new base A (3/4);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.14e-01

ACGGTTTCCTGGTGGCTCGTTCATCTGACCGTAGACCAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCTTGATGCCGGTTTCCAGCAGGTCGTTGCCGCCTGCCTG  >  NC_002947/6175952‑6176245
                                                                                                                                                    |                                                                                                                                                 
aCGGTTTCCTGGTGGCTCGTTCATCTGACCGTAGACCAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCATACCAGCGAACACGGAGTAACCGCTGTGTTCCATgg                                                                                                                                                   <  4:20306/149‑1 (MQ=255)
  ggTTTCCTGGTGGCTCGTTCATCTGACCGTAGACCAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGc                                                                                                                                                  >  6:11436/1‑148 (MQ=255)
    tttCCTGGTGGCTCGTTCATCTGACCGTAGACCAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGAt                                                                                                                                               <  6:353938/149‑1 (MQ=255)
        cTGGTGGCTCGTTCATCTGACCGTAGACCAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTa                                                                                                                                           <  3:159981/149‑1 (MQ=255)
        cTGGTGGCTCGTTCATCTGACCGTAGACCAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTa                                                                                                                                           <  6:118785/149‑1 (MQ=255)
           gtggCTCGTTCATCTGACCGTAGACCAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACg                                                                                                                                         >  7:35329/1‑148 (MQ=255)
              gCTCGTTCATCTGACCGTAGACCAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGAt                                                                                                                                      <  7:137717/148‑1 (MQ=255)
                tCGTTCATCTGACCGTAGACCAGCGCTACATTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTAGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAAACGCTGTGTTCCATGGCGATGTTACGGATCAg                                                                                                                                   >  1:201653/1‑149 (MQ=255)
                           aCCGTAGACCAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGt                                                                                                                        <  5:276370/149‑1 (MQ=255)
                              gTAGACCAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTa                                                                                                                     <  8:15557/149‑1 (MQ=255)
                                 gACCAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCATCACGAGTACGCTCACCCACACCAGCGAAAACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACgg                                                                                                                  >  7:67815/1‑149 (MQ=255)
                                    cAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACAAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCt                                                                                                               >  5:321714/1‑149 (MQ=255)
                                    cAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCAACCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCAGCATGTTTACTGtat                                                                                                               >  8:313155/1‑147 (MQ=255)
                                     aGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCtt                                                                                                              <  1:232963/149‑1 (MQ=255)
                                       cgcTACTTTGTCGAGAACGTTGGAGTCCTTCATATCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGc                                                                                                            <  1:238525/149‑1 (MQ=255)
                                         cTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGcc                                                                                                           >  1:156050/1‑148 (MQ=255)
                                          tACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGa                                                                                                         >  4:65451/1‑149 (MQ=255)
                                                gTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCg                                                                                                    <  6:321714/148‑1 (MQ=255)
                                                 tCGAGAACGTTGGAGTCCTTCATCTCGTGTTATAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCTAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGc                                                                                                  <  5:146962/149‑1 (MQ=255)
                                                 tCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTACCCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCgg                                                                                                   >  8:171590/1‑148 (MQ=255)
                                                 tCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTACCCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGc                                                                                                  <  7:171590/149‑1 (MQ=255)
                                                 tCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCgg                                                                                                   <  8:35329/148‑1 (MQ=255)
                                                 tCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCgg                                                                                                   <  7:234678/148‑1 (MQ=255)
                                                     gaACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGcacca                                                                                              <  3:65451/149‑1 (MQ=255)
                                                      aaCGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGcaccac                                                                                             >  5:304878/1‑149 (MQ=255)
                                                        cGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGcc                                                                                                           <  6:303614/133‑1 (MQ=255)
                                                        cGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGcc                                                                                                           >  5:303614/1‑133 (MQ=255)
                                                         gTTTGAGTCCTTCATGTCGTTGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCgg                                                                                                   <  8:30118/140‑1 (MQ=255)
                                                         gTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGTTCTTGCCGACACCgg                                                                                                   >  7:30118/1‑140 (MQ=255)
                                                           tGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGcacca                                                                                              <  7:135156/143‑1 (MQ=255)
                                                           tGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGcacca                                                                                              >  8:135156/1‑143 (MQ=255)
                                                            ggAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAAc                                                                                        <  8:67815/148‑1 (MQ=255)
                                                               gTCCTTCATCTCGTTGTAGAAGTCGTTACCCTCACTAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGACGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGa                                                                                     <  7:47144/148‑1 (MQ=255)
                                                                   ttCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGCCACCGCCACCCCCGAACAGACCAAc                                                                                >  5:331196/1‑149 (MQ=255)
                                                                         tcGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGa                                                                                          <  4:337774/133‑1 (MQ=255)
                                                                         tcGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGa                                                                                          >  3:337774/1‑133 (MQ=255)
                                                                         tcGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGACGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTac                                                                           >  1:205072/1‑148 (MQ=255)
                                                                           gTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGACGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTaccac                                                                        >  7:60634/1‑149 (MQ=255)
                                                                              gTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGAc                                                                                    <  5:83961/134‑1 (MQ=255)
                                                                              gTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGAc                                                                                    >  6:83961/1‑134 (MQ=255)
                                                                              gTAGAAGGCTGTACCCTAACGAGTACGCTGACCCACACCAGCGAACACGGAGTAACGGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTAGCACCCt                                                                     <  6:331196/149‑1 (MQ=255)
                                                                               tAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCt                                                                     <  5:11436/148‑1 (MQ=255)
                                                                                        gtACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACgg                                                           <  6:219225/148‑1 (MQ=255)
                                                                                             cTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCaaa                                                      >  6:210817/1‑149 (MQ=255)
                                                                                              tCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACgg                                                           <  6:188978/143‑1 (MQ=255)
                                                                                              tCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACgg                                                           >  5:188978/1‑143 (MQ=255)
                                                                                              tCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAAc                                                     >  2:130908/1‑149 (MQ=255)
                                                                                                aCGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAAc                                                     >  1:242982/1‑147 (MQ=255)
                                                                                                 cGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAg                                                  <  1:130908/149‑1 (MQ=255)
                                                                                                  gAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTgg                                                                  >  3:72909/1‑132 (MQ=255)
                                                                                                  gAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTgg                                                                  <  4:72909/132‑1 (MQ=255)
                                                                                                   aGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGt                                                <  6:304878/149‑1 (MQ=255)
                                                                                                          tCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAAc                                         >  2:176848/1‑149 (MQ=255)
                                                                                                             cccACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCtt                                      <  2:156050/149‑1 (MQ=255)
                                                                                                             cccACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGACGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCtt                                      <  8:60634/149‑1 (MQ=255)
                                                                                                                  acCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCTTGATGc                                 >  1:214778/1‑149 (MQ=255)
                                                                                                                  acCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCTTGATGc                                 >  3:55986/1‑149 (MQ=255)
                                                                                                                          acacGGAGTAACCGCTGTGTTCCATGACGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATaa                                          <  7:281870/132‑1 (MQ=255)
                                                                                                                          acacGGAGTAACCGCTGTGTTCCATGACGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGAACAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATaa                                          >  8:281870/1‑132 (MQ=255)
                                                                                                                            acGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCTTGATGCCGGTTTCcag                       >  1:344802/1‑149 (MQ=255)
                                                                                                                            acGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCTTGATGCCGGTTTCcag                       >  8:13905/1‑149 (MQ=255)
                                                                                                                                        cTGTGTTCCATGACGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCTTGATGCCGGTTTCCAGCAGGTCGTTgcc           <  2:205072/149‑1 (MQ=255)
                                                                                                                                              tCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCTTGATGCCGGTTTCCAGCAGGTCGTTGCCgcctgc     >  1:217547/1‑149 (MQ=255)
                                                                                                                                               ccATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCTTGATGCCGGTTTCCAGCAGGTCGTTGCCgcctgcc    >  1:6328/1‑149 (MQ=255)
                                                                                                                                               ccATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCTTGATGCCGGTTTCCAGCAGGTCGTTGCCgcctgcc    >  1:40353/1‑149 (MQ=255)
                                                                                                                                               ccATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCTTGATGCCGGTTTCCAGCAGGTCGTTGCCgcctgcc    >  1:11868/1‑149 (MQ=255)
                                                                                                                                                 aTGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCTTGATGCCGGTTTCCAGCAGGTCGTTGCCGcctgcctg  <  2:201653/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
ACGGTTTCCTGGTGGCTCGTTCATCTGACCGTAGACCAGCGCTACTTTGTCGAGAACGTTGGAGTCCTTCATCTCGTGGTAGAAGTCGTTACCCTCACGAGTACGCTCACCCACACCAGCGAACACGGAGTAACCGCTGTGTTCCATGGCGATGTTACGGATCAGTTCCATCATGTTTACGGTCTTGCCGACACCGGCACCACCGAACAGACCAACCTTACCACCCTTGGCGAACGGGCAAACCAGGTCGATAACCTTGATGCCGGTTTCCAGCAGGTCGTTGCCGCCTGCCTG  >  NC_002947/6175952‑6176245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑