Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,177,515 C→T 6.6% intergenic (‑31/+18) atpG ← / ← atpA ATP synthase subunit gamma/ATP synthase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,177,5150CT 6.6% 222.0 / 3.7 75intergenic (‑31/+18)atpG/atpAATP synthase subunit gamma/ATP synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (37/33);  new base T (3/2);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

GCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCGTGATCACGGTTGAAGAAGGC  >  NC_002947/6177371‑6177658
                                                                                                                                                |                                                                                                                                               
gcgcATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCGATCAGGTTAGCAAGCGGGGGCCTGAg                                                                                                                                             >  3:51444/1‑149 (MQ=255)
           ccTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCTCAGGCCCCCGCttg                                                                                                                                   <  6:182612/148‑3 (MQ=255)
           ccTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGc                                                                                                                                   <  5:75821/148‑1 (MQ=255)
           ccTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCg                                                                                                                                  >  3:60720/1‑149 (MQ=255)
            cTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCgg                                                                                                                                 <  3:132404/149‑1 (MQ=255)
            cTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGACATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCgg                                                                                                                                 >  4:105603/1‑149 (MQ=255)
                cgcATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTa                                                                                                                             <  3:184136/149‑1 (MQ=255)
                    tCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAg                                                                                                                         >  2:116528/1‑149 (MQ=255)
                      ttGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGt                                                                                                                       >  6:158213/1‑149 (MQ=255)
                        gCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCt                                                                                                                     >  5:10787/1‑149 (MQ=255)
                           gACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTggg                                                                                                                  >  4:17031/1‑149 (MQ=255)
                             ccGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTg                                                                                                                <  6:10787/149‑1 (MQ=255)
                              cGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCTCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCAATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTgg                                                                                                               <  4:51444/149‑1 (MQ=255)
                              cGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCTCAGGCCCCCGCtt                                                                                                                                    <  7:305704/128‑2 (MQ=255)
                              cGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCTCAGGCCCCCGCtt                                                                                                                                    >  8:305704/1‑127 (MQ=255)
                              cGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTgg                                                                                                               >  5:24676/1‑149 (MQ=255)
                              cGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTgg                                                                                                               >  8:280334/1‑149 (MQ=255)
                                ccACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTAcc                                                                                                                           >  5:146230/1‑135 (MQ=255)
                                ccACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTAcc                                                                                                                           <  6:146230/135‑1 (MQ=255)
                                       atgcATGGCTCTGGTATTTTTTTGCGTTCTTTTGAGTCGCGCAATATTACTGCTAATCTCTTTTGCGCCTGCCATGTATCACCTATCTGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACt                                                                                                      <  6:24676/146‑1 (MQ=255)
                                           aTGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTtctc                                                                                                  <  3:42506/149‑1 (MQ=255)
                                                 caggTTATTTTTTGCGTGCGTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGAt                                                                                               <  6:242406/144‑1 (MQ=255)
                                                 cTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCTGGGGCCTGAGACCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGAt                                                                                               >  5:242406/1‑146 (MQ=255)
                                                    gTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGc                                                                                         >  6:197864/1‑149 (MQ=255)
                                                    gTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGACTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGTGTGGCCTTGAACTTCTCGATACCg                                                                                           <  3:207814/147‑1 (MQ=255)
                                                        ttttttGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCttt                                                                                      >  3:332120/1‑148 (MQ=255)
                                                            ggctgTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGc                                                                                 <  8:177440/145‑1 (MQ=255)
                                                             tGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGc                                                                                 <  4:332120/148‑1 (MQ=255)
                                                                gTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGAGGCTTACCAGGTCTGGGTGGCCTTGAAGTTCTCGATACCGGCTTTCAGGCCTgg                                                                             >  6:289310/1‑148 (MQ=255)
                                                                    ttttGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTc                                                                          >  4:286135/1‑148 (MQ=255)
                                                                             tCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTAcc                                                                                                                           >  2:61810/1‑90 (MQ=255)
                                                                             tCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTAcc                                                                                                                           <  1:61810/90‑1 (MQ=255)
                                                                              cGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTcgtcgt                                                               >  4:152610/1‑149 (MQ=255)
                                                                               gCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTcgtcgt                                                               <  5:223650/148‑1 (MQ=255)
                                                                               gCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGtt                                                              <  3:17031/149‑1 (MQ=255)
                                                                                 aaTCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTg                                                             >  8:104809/1‑148 (MQ=255)
                                                                                         tGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCAccc                                                    >  3:157711/1‑149 (MQ=255)
                                                                                         tGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCAccc                                                    >  2:252063/1‑149 (MQ=255)
                                                                                         tGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGACCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACAGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCAccc                                                    >  7:174964/1‑149 (MQ=255)
                                                                                            gAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTc                                                 <  4:157711/149‑1 (MQ=255)
                                                                                                   ttttGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACg                                              <  4:60720/145‑1 (MQ=255)
                                                                                                     ttGCGCCTGCCATGTATCACCTATCAGGTTAGCACGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGAt                                         <  7:291251/148‑1 (MQ=255)
                                                                                                     ttGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTGTGGGTGGCGTTGAAGTTGTCGATACCGGCTTGCAGGCGTGCGTGGATTGGGGCGTTGACGTCACCCTTCACGTTGAtg                                        >  3:217796/1‑148 (MQ=255)
                                                                                                     ttGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTg                                           <  3:152610/146‑1 (MQ=255)
                                                                                                      tGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATc                                        <  5:197864/148‑1 (MQ=255)
                                                                                                         gcCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGt                                             >  8:297741/1‑140 (MQ=255)
                                                                                                         gcCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGt                                             <  7:297741/140‑1 (MQ=255)
                                                                                                          ccTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGc                                   <  5:79979/149‑1 (MQ=255)
                                                                                                              ccATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCa                                 <  8:174964/147‑1 (MQ=255)
                                                                                                              ccATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCAt                                <  3:105603/148‑1 (MQ=255)
                                                                                                               cATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCa                              <  1:116528/149‑1 (MQ=255)
                                                                                                                aTGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGc                                                                                                                                >  1:8614/1‑50 (MQ=255)
                                                                                                                aTGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGc                                                                                                                                <  2:8614/50‑1 (MQ=255)
                                                                                                                 tGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCa                              <  2:260901/147‑1 (MQ=255)
                                                                                                                   tATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGc                                                                             >  6:308325/1‑98 (MQ=255)
                                                                                                                   tATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGc                                                                             <  5:308325/98‑1 (MQ=255)
                                                                                                                    aTCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTc                                     >  8:236079/1‑137 (MQ=255)
                                                                                                                    aTCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTc                                     <  7:236079/137‑1 (MQ=255)
                                                                                                                    aTCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGtt                           >  3:213260/1‑147 (MQ=255)
                                                                                                                    aTCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCATTCACGTTGATCTTCGCCATCAGtt                           >  4:52541/1‑147 (MQ=255)
                                                                                                                      cACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGc                       >  1:36531/1‑149 (MQ=255)
                                                                                                                       aCCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCg                      >  3:3867/1‑149 (MQ=255)
                                                                                                                       aCCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCg                      >  8:217228/1‑149 (MQ=255)
                                                                                                                        ccTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCGt                     >  6:47164/1‑149 (MQ=255)
                                                                                                                        ccTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCGt                     >  6:42049/1‑149 (MQ=255)
                                                                                                                           aTCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCGTGAt                  >  4:231687/1‑149 (MQ=255)
                                                                                                                                  tAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCAt                                >  3:116543/1‑128 (MQ=255)
                                                                                                                                  tAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCAt                                <  4:116543/128‑1 (MQ=255)
                                                                                                                                   ccgcaGCGGGGGCTCAGGCCCCCGCTGAGGCTTACCAGGTGTGGGTGGCCGTGAACTTATCGATACCGGCTTTCAGGCCAGCGTAGATTTAGTAGTTGAATTAACCCATCACCTTGATAGTAGACAAAAGTTCAGCGTGATGACGGtt           >  8:286145/5‑148 (MQ=255)
                                                                                                                                   aGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCGTGATCACGGtt           >  8:97142/1‑148 (MQ=255)
                                                                                                                                    gCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCGTGATCACGGTTga         <  4:3867/149‑1 (MQ=255)
                                                                                                                                     gcaGCGGGGGCTCAGGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCGTGATCACGGTTg          >  6:307055/3‑147 (MQ=255)
                                                                                                                                       aGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCGTGATCACGGTTgaaga      <  1:252063/149‑1 (MQ=255)
                                                                                                                                          gggggCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCGTGATCACGGTTGAAGAAgg   <  3:286135/149‑1 (MQ=255)
                                                                                                                                            gggCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCGTGATCACGGTTGAAGAAGGc  >  6:301187/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
GCGCATTTGTGCCTTGCGCATCTTGCTGACCGCCACTTTCTCCATGGCGCTGGTAATTTTTTGCGTGCTTTTGATGCTCGCAATCTTACTGCGAATCTCTTTTGCGCCTGCCATGTATCACCTATCAGGTTAGCAAGCGGGGGCCTGAGCCCCCGCTGCGGCTTACCAGGTCTGGGTGGCCTTGAACTTCTCGATACCGGCTTTCAGGCCTGCGTCGATTTCGTCGTTGAAGTCACCCTTCACGTTGATCTTCGCCATCAGTTCAGCGTGATCACGGTTGAAGAAGGC  >  NC_002947/6177371‑6177658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑