Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 92,189 A→C 5.6% I64S (ATC→AGC)  trpA ← tryptophan synthase subunit alpha

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294792,1890AC 5.6% 228.7 / 3.6 71I64S (ATC→AGC) trpAtryptophan synthase subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (35/32);  new base C (2/2);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

TGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGAGCGG  >  NC_002947/92043‑92335
                                                                                                                                                  |                                                                                                                                                  
tgtCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCCTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATg                                                                                                                                                  <  7:147743/149‑1 (MQ=255)
 gtCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATgg                                                                                                                                                 >  8:83987/1‑149 (MQ=255)
  tCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATgg                                                                                                                                                 <  6:353223/148‑1 (MQ=255)
    cACGCCTAAGGGGTGGATAGGGTTGTAGTAGCCCATCAGCACGTTCTGCGTGGTCTGGTTATCCACACGGAATTCGCGATCCATCTGCAGGGGCTTGAGCATGGTCTGGCCAGCTTGAAGGGCGCGCAGGGTGGCCATCCAGCTGGCCa                                                                                                                                              <  7:169173/149‑1 (MQ=255)
    cACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCa                                                                                                                                              <  8:101792/149‑1 (MQ=255)
     aCGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCAc                                                                                                                                             >  7:291596/1‑149 (MQ=255)
     aCGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCAc                                                                                                                                             <  6:147124/149‑1 (MQ=255)
       gCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgc                                                                                                                                           >  6:314511/1‑149 (MQ=255)
        ccAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACgc                                                                                                                                           >  5:218264/1‑148 (MQ=255)
                     tAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCggg                                                                                                                             <  6:218264/149‑1 (MQ=255)
                        ggTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCg                                                                                                                          >  5:307892/1‑149 (MQ=255)
                              agtagCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTg                                                                                                                       <  6:268451/146‑1 (MQ=255)
                              agtagCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTg                                                                                                                       >  5:268451/1‑146 (MQ=255)
                              agtagCCCATCAGCACGATCGGCGTGGGCTGGTTAGCAACACGGAATTCGCGAAGCATCGGAAGGGTCTTCACCAAGGTCTGGCCCGATTCAAGGGCGGCCAGGGTGGCCAGCTGGATGGCCACGCCTTCGGCCAGCGGGTCGGTGAAc                                                                                                                    >  4:212365/1‑149 (MQ=255)
                               gtagCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAAc                                                                                                                    <  1:307860/148‑1 (MQ=255)
                                tagCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACgg                                                                                                                  >  6:73015/1‑149 (MQ=255)
                                    ccATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATg                                                                                                              >  4:259584/1‑149 (MQ=255)
                                      aTCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGcc                                                                                                            >  1:223115/1‑149 (MQ=255)
                                      aTCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGcc                                                                                                            <  2:223115/149‑1 (MQ=255)
                                              gATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATg                                                                                                              <  3:80035/139‑1 (MQ=255)
                                              gATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATg                                                                                                              >  4:80035/1‑139 (MQ=255)
                                                tCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATc                                                                                                  <  7:83987/149‑1 (MQ=255)
                                                   gCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCt                                                                                                       >  8:133412/1‑141 (MQ=255)
                                                   gCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCt                                                                                                       <  7:133412/141‑1 (MQ=255)
                                                           tGGTTATACACACGCAATTCGCGAACCATCTGCAGGGTCTTCACCAAGGTCTGGCCAGCTTAAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGATGAACGGTATGCCCAGCTCGATCACGTCGGCGcc                                                                                       >  4:281634/1‑149 (MQ=255)
                                                             gTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCca                                                                                      <  8:291596/148‑1 (MQ=255)
                                                                    acacGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGccag                                                                              >  5:99046/1‑147 (MQ=255)
                                                                    acacGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGccag                                                                              <  6:99046/149‑3 (MQ=255)
                                                                    acacGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCgg                                                                              >  6:1823/1‑149 (MQ=255)
                                                                    acacGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCgg                                                                              >  3:286925/1‑149 (MQ=255)
                                                                    acacGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCgg                                                                              >  6:6672/1‑149 (MQ=255)
                                                                      acGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCATGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGc                                                                             <  1:283147/148‑1 (MQ=255)
                                                                      acGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGc                                                                             >  2:283147/1‑148 (MQ=255)
                                                                               gcgAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGa                                                                   <  5:1823/149‑1 (MQ=255)
                                                                               gcgAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGa                                                                   <  3:259584/149‑1 (MQ=255)
                                                                               gcgAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGa                                                                   <  5:6672/149‑1 (MQ=255)
                                                                                cgAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCATGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAg                                                                  >  8:205958/1‑149 (MQ=255)
                                                                                  aaCCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGt                                                                                                                            >  3:233038/1‑89 (MQ=255)
                                                                                  aaCCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGt                                                                                                                            <  4:233038/89‑1 (MQ=255)
                                                                                     cATCTGCATGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCTGAGTGTGGCCAGCTGGATGGCCACGGCATCGGCCATCGGTTCTGTGAACGGCATGCCCAGCTCGATCAAGTCGTCTCCAGCATACTGCAGGCCATTGAGGATCt                                                             <  4:306189/149‑1 (MQ=255)
                                                                                     cATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCt                                                             >  6:315442/1‑149 (MQ=255)
                                                                                         tGCAGGGTCTTGACCAAAGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCaa                                                         >  8:39300/1‑149 (MQ=255)
                                                                                           cAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAAc                                                        <  5:314511/148‑1 (MQ=255)
                                                                                            aGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAAc                                                        >  5:265879/1‑147 (MQ=255)
                                                                                                tCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGcc                                                                                       <  7:252342/112‑1 (MQ=255)
                                                                                                tCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGcc                                                                                       >  8:252342/1‑112 (MQ=255)
                                                                                                tCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGa                                                      >  3:189769/1‑145 (MQ=255)
                                                                                                tCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGa                                                      <  4:189769/145‑1 (MQ=255)
                                                                                                tCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGcg                                                  >  6:76551/1‑149 (MQ=255)
                                                                                                 cTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTg                                                            >  6:353993/1‑138 (MQ=255)
                                                                                                 cTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTg                                                            <  5:353993/138‑1 (MQ=255)
                                                                                                      ccAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAg                                            >  5:338470/1‑149 (MQ=255)
                                                                                                         aGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGccc                                         <  4:247407/149‑1 (MQ=255)
                                                                                                           gTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCgg                                       >  6:64703/1‑149 (MQ=255)
                                                                                                                gCCAGCTTCAAGGTCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACCGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTc                                    <  3:212365/147‑1 (MQ=255)
                                                                                                                gCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGc                                  >  3:25264/1‑149 (MQ=255)
                                                                                                                      ttCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGccc                                         >  2:301552/1‑136 (MQ=255)
                                                                                                                      ttCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGccc                                         <  1:301552/136‑1 (MQ=255)
                                                                                                                          aGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGc                                                   <  2:286372/122‑1 (MQ=255)
                                                                                                                             gcgcgcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACg                       >  6:135829/1‑147 (MQ=255)
                                                                                                                              cgcgcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAg                    <  5:76551/149‑1 (MQ=255)
                                                                                                                              cgcgcAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGGGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAg                    <  6:307892/149‑1 (MQ=255)
                                                                                                                                   aGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGAc               >  2:226487/1‑149 (MQ=255)
                                                                                                                                    gggTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGAcc              <  5:135829/149‑1 (MQ=255)
                                                                                                                                     ggTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGAc               >  7:309845/1‑147 (MQ=255)
                                                                                                                                      gTGGCCATCCAGCTGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTg                 <  1:199475/144‑1 (MQ=255)
                                                                                                                                      gTGGCCATCCAGCTGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTg                 >  2:199475/1‑144 (MQ=255)
                                                                                                                                      gTGGCCATCCAGCTGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAg            >  4:93401/1‑149 (MQ=255)
                                                                                                                                        ggCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTg          <  6:265879/149‑1 (MQ=255)
                                                                                                                                           cAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGcc        >  4:330290/1‑148 (MQ=255)
                                                                                                                                                 gATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGAGcgg  <  4:286925/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGTCCACGCCAAAGCGGTGGATAGGGTTGTAGTAGCCCATCAGCACGATCGGCGTGGTCTGGTTATCCACACGGAATTCGCGAACCATCTGCAGGGTCTTGACCAAGGTCTGGCCAGCTTCAAGGGCGCGCAGGGTGGCCAGCTGGATGGCCACGCCATCGGCCATCGGGTCGGTGAACGGCATGCCCAGCTCGATCACGTCGGCGCCAGCAGCCGGCAGGCCCTTGAGGATCTGCAACGAGGCGTCGTAGCCCGGGTCGCCAGCTGTGACGAAGGTGACCAGTGCCGAGCGG  >  NC_002947/92043‑92335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑